##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AclI AgeI AocI Aor51HI AscI
AviII BbeI BbrPI BbvCI BsiEI
BsiWI BspDI BspHI BspLU11I BspMI
BsrBI BsrDI BssHII BstBI BstEII
BstPI Bsu36I ClaI CpoI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV FseI FspI HpaI KasI
KspI MluI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SpeI SphI Sse8387I XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 69 enzymes) #
###################################
AatII 1412 GACGTC GACGT^C
Acc65I 2755 GGTACC G^GTACC
AccI 3580 GTATAC GT^[AC][GT]AC
AccIII 531 TCCGGA T^CCGGA
AflII 1657 CTTAAG C^TTAAG
AflIII 3435 ACGTGT A^C[GA][CT]GT
AhdI 1209 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3310 GTGCAC G^TGCAC
ApaI 3058 GGGCCC GGGCC^C
ApaLI 3310 GTGCAC G^TGCAC
Asp700I 2067 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2755 GGTACC G^GTACC
AspEI 1209 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1029 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2222 CCTAGG C^CTAGG
BciVI 2546 GGATAC GTATCC(6/5)
BclI 731 TGATCA T^GATCA
BfrI 1657 CTTAAG C^TTAAG
BlnI 2222 CCTAGG C^CTAGG
BlpI 360 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 360 GCTGAGC GC^T[ACGT]AGC
BsaBI 3073 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3951 GGTCTC GGTCTC(1/5)
BsaWI 531 TCCGGA [AT]^CCGG[AT]
BseAI 531 TCCGGA T^CCGGA
BsgI 3341 CTGCAC GTGCAG(16/14)
BsmI 3274 GAATGC GAATGC(1/-1)
BspEI 531 TCCGGA T^CCGGA
BsrFI 774 GCCGGT [GA]^CCGG[CT]
BssSI 579 CACGAG CACGAG(-5/-1)
Bst1107I 3580 GTATAC GTA^TAC
BstAPI 222 GCAGTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3580 GTATAC GTA^TAC
CelII 360 GCTGAGC GC^T[ACGT]AGC
CfoI 1087 GCGC GCG^C
Cfr10I 774 GCCGGT [GA]^CCGG[CT]
Eam1105I 1209 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3062 CCTTATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 280 GAATTC G^AATTC
EcoT14I 2222 CCTAGG C^C[AT][AT]GG
EcoT22I 3514 ATGCAT ATGCA^T
FbaI 731 TGATCA T^GATCA
HaeII 1086 AGCGCC [GA]GCGC^[CT]
HhaI 1087 GCGC GCG^C
HinP1I 1087 GCGC G^CGC
HincII 1674 GTTGAC GT[CT]^[GA]AC
HindII 1674 GTTGAC GT[CT]^[GA]AC
KpnI 2755 GGTACC GGTAC^C
MamI 3073 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 531 TCCGGA T^CCGGA
NheI 2111 GCTAGC G^CTAGC
NsiI 3514 ATGCAT ATGCA^T
PflFI 1029 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 311 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 139 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 102 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1377 AGTACT AGT^ACT
SmaI 136 CCCGGG CCC^GGG
SnaBI 3591 TACGTA TAC^GTA
SspI 3610 AATATT AAT^ATT
StyI 2222 CCTAGG C^C[AT][AT]GG
SwaI 3613 ATTTAAAT ATTT^AAAT
TaqI 268 TCGA T^CGA
Tth111I 1029 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 268 TCGA T^CGA
Van91I 311 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 136 CCCGGG C^CCGGG
XmaI 136 CCCGGG C^CCGGG
XmnI 2067 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 474 CGCG CG^CG
541 CGCG
AvaI 136 CCCGGG C^[CT]CG[GA]G
1152 CTCGGG
BamHI 465 GGATCC G^GATCC
1156 GGATCC
BbsI 165 GAAGAC GAAGAC(2/6)
697 GAAGAC
BcgI 1214 CGAGTCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1109 GCACCGACATCG
BglI 655 GCCTTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
820 GCCTTCCTGGC
BglII 848 AGATCT A^GATCT
3627 AGATCT
BpuAI 165 GAAGAC GAAGAC(2/6)
697 GAAGAC
BsaAI 3434 TACGTG [CT]AC^GT[GA]
3591 TACGTA
BsmBI 527 CGTCTC CGTCTC(1/5)
837 CGTCTC
BsoBI 136 CCCGGG C^[CT]CG[GA]G
1152 CTCGGG
BstUI 474 CGCG CG^CG
541 CGCG
BstXI 770 CCAGGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1067 CCAAAGGCTTGG
DrdI 757 GACAAGCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3149 GACACTACTGTC
DsaI 710 CCGTGG C^C[GA][CT]GG
1462 CCACGG
EarI 961 CTCTTC CTCTTC(1/4)
1043 CTCTTC
FauI 1092 CCCGC CCCGC(4/6)
3282 CCCGC
HindIII 1728 AAGCTT A^AGCTT
2813 AAGCTT
Ksp632I 961 CTCTTC CTCTTC(1/4)
1043 CTCTTC
MfeI 1956 CAATTG C^AATTG
3023 CAATTG
MspA1I 1093 CCGCTG C[AC]G^C[GT]G
1267 CCGCTG
MunI 1956 CAATTG C^AATTG
3023 CAATTG
MvnI 474 CGCG CG^CG
541 CGCG
SfaNI 2149 GATGC GCATC(5/9)
3036 GCATC
SmlI 1657 CTTAAG C^T[CT][GA]AG
3300 CTCAAG
StuI 606 AGGCCT AGG^CCT
2520 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaII BalI BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII DraIII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HgaI Hin1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PshBI PstI RsaI Sau3AI
Sau96I ScrFI SfcI SspBI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XbaI XhoII