##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsiWI BsmI
Bsp1407I BspDI BspLU11I BsrGI BssHII
BstAPI BstBI BstEII BstPI CelII
ClaI CpoI Eam1105I Eco47III EcoO65I
EcoT22I FseI FspI HincII HindII
HpaI KpnI KspI MamI MfeI
MluI MunI NaeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI ScaI
SfuI SgrAI SmaI SnaBI SphI
SspBI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 1478 GACGTC GACGT^C
AccIII 1920 TCCGGA T^CCGGA
AflIII 2411 ACACGT A^C[GA][CT]GT
BbeI 606 GGCGCC GGCGC^C
BbsI 2772 GTCTTC GAAGAC(2/6)
BcgI 1945 CGAGGCTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1129 GGATAC GTATCC(6/5)
BclI 1593 TGATCA T^GATCA
BpuAI 2772 GTCTTC GAAGAC(2/6)
BsaWI 1920 TCCGGA [AT]^CCGG[AT]
BseAI 1920 TCCGGA T^CCGGA
BsmBI 190 CGTCTC CGTCTC(1/5)
BspEI 1920 TCCGGA T^CCGGA
BsrFI 1992 GCCGGT [GA]^CCGG[CT]
BssSI 1943 CACGAG CACGAG(-5/-1)
Bst1107I 2150 GTATAC GTA^TAC
BstZ17I 2150 GTATAC GTA^TAC
Cfr10I 1992 GCCGGT [GA]^CCGG[CT]
DraI 1472 TTTAAA TTT^AAA
DraIII 1196 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1478 GACGTCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2658 GAATTC G^AATTC
EcoRV 2483 GATATC GAT^ATC
FbaI 1593 TGATCA T^GATCA
HgaI 49 GCGTC GACGC(5/10)
KasI 606 GGCGCC G^GCGCC
MroI 1920 TCCGGA T^CCGGA
NarI 606 GGCGCC GG^CGCC
NdeI 2597 CATATG CA^TATG
NheI 2720 GCTAGC G^CTAGC
PshAI 634 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 383 CCTGCAGG CCTGCA^GG
SfiI 1982 GGCCACTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 606 GGCGCC GGC^GCC
SmlI 1180 CTTGAG C^T[CT][GA]AG
SpeI 670 ACTAGT A^CTAGT
Sse8387I 383 CCTGCAGG CCTGCA^GG
SspI 1373 AATATT AAT^ATT
TaqI 1336 TCGA T^CGA
TthHB8I 1336 TCGA T^CGA
XcmI 567 CCACCACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 2150 GTATAC GT^[AC][GT]AC
2645 GTCTAC
AfaI 460 GTAC GT^AC
2626 GTAC
Alw44I 674 GTGCAC G^TGCAC
1247 GTGCAC
ApaLI 674 GTGCAC G^TGCAC
1247 GTGCAC
AvaI 193 CTCGGG C^[CT]CG[GA]G
636 CTCGGG
BglII 1710 AGATCT A^GATCT
2011 AGATCT
BsgI 2456 CTGCAC GTGCAG(16/14)
554 GTGCAG
BsiEI 114 CGGCCG CG[GA][CT]^CG
247 CGGCCG
BsoBI 193 CTCGGG C^[CT]CG[GA]G
636 CTCGGG
BspHI 2880 TCATGA T^CATGA
3324 TCATGA
BsrBI 140 GAGCGG CCGCTC(-3/-3)
2357 GAGCGG
BsrDI 2335 CATTGC GCAATG(2/0)
3468 GCAATG
EagI 114 CGGCCG C^GGCCG
247 CGGCCG
EclXI 114 CGGCCG C^GGCCG
247 CGGCCG
Eco52I 114 CGGCCG C^GGCCG
247 CGGCCG
HindIII 599 AAGCTT A^AGCTT
1621 AAGCTT
NspI 1146 GCATGT [GA]CATG^[CT]
1290 ACATGC
PpuMI 196 GGGTCCC [GA]G^G[AT]CC[CT]
1299 GGGACCT
PvuII 530 CAGCTG CAG^CTG
1970 CAGCTG
RcaI 2880 TCATGA T^CATGA
3324 TCATGA
RsaI 460 GTAC GT^AC
2626 GTAC
SexAI 1197 ACCAGGT A^CC[AT]GGT
1242 ACCTGGT
StuI 838 AGGCCT AGG^CCT
2840 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AcyI AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII
XmnI