##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI AscI Asp718I AspEI AspI
AviII AvrII BalI BanI BbeI
BbrPI BbvCI BcgI BciVI BcnI
BlnI BlpI Bpu1102I BsaWI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco52I EcoNI
EcoRI FseI FspI KasI KpnI
KspI MluI MluNI MroI MscI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SwaI Tth111I Van91I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 893 GTAGAC GT^[AC][GT]AC
AcyI 59 GACGCC G[GA]^CG[CT]C
AocI 3753 CCTAAGG CC^T[ACGT]AGG
Aor51HI 2296 AGCGCT AGC^GCT
ApaI 1144 GGGCCC GGGCC^C
AvaI 2801 CTCGAG C^[CT]CG[GA]G
BclI 211 TGATCA T^GATCA
BsaBI 1988 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 59 GACGCC G[GA]^CG[CT]C
BsaI 84 GAGACC GGTCTC(1/5)
BsmBI 57 GAGACG CGTCTC(1/5)
BsoBI 2801 CTCGAG C^[CT]CG[GA]G
BspMI 1478 GCAGGT ACCTGC(4/8)
BstEII 2841 GGTCACC G^GT[ACGT]ACC
BstPI 2841 GGTCACC G^GT[ACGT]ACC
Bsu36I 3753 CCTAAGG CC^T[ACGT]AGG
CfoI 2297 GCGC GCG^C
DsaI 1683 CCATGG C^C[GA][CT]GG
EarI 1157 GAAGAG CTCTTC(1/4)
Eco47III 2296 AGCGCT AGC^GCT
Eco81I 3753 CCTAAGG CC^T[ACGT]AGG
EcoO65I 2841 GGTCACC G^GT[ACGT]ACC
EcoRV 2476 GATATC GAT^ATC
EcoT22I 3572 ATGCAT ATGCA^T
FbaI 211 TGATCA T^GATCA
HaeII 2296 AGCGCT [GA]GCGC^[CT]
HapII 81 CCGG C^CGG
HgaI 59 GACGC GACGC(5/10)
HhaI 2297 GCGC GCG^C
Hin1I 59 GACGCC G[GA]^CG[CT]C
HinP1I 2297 GCGC G^CGC
HpaII 81 CCGG C^CGG
Ksp632I 1157 GAAGAG CTCTTC(1/4)
MamI 1988 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2212 CAATTG C^AATTG
MspI 81 CCGG C^CGG
MunI 2212 CAATTG C^AATTG
NcoI 1683 CCATGG C^CATGG
NdeI 630 CATATG CA^TATG
NsiI 3572 ATGCAT ATGCA^T
PaeR7I 2801 CTCGAG C^TCGAG
PshAI 1292 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1929 GAGCTC GAGCT^C
SapI 1157 GAAGAGC GCTCTTC(1/4)
ScaI 548 AGTACT AGT^ACT
SphI 1162 GCATGC GCATG^C
StuI 618 AGGCCT AGG^CCT
XcmI 2566 CCAGTTTGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2801 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 9 CGCG CG^CG
112 CGCG
AflII 2849 CTTAAG C^TTAAG
3780 CTTAAG
Alw44I 464 GTGCAC G^TGCAC
1482 GTGCAC
AlwNI 2315 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
3391 CAGCAACTG
ApaLI 464 GTGCAC G^TGCAC
1482 GTGCAC
AseI 1259 ATTAAT AT^TAAT
3862 ATTAAT
AsnI 1259 ATTAAT AT^TAAT
3862 ATTAAT
AvaII 347 GGACC G^G[AT]CC
1034 GGTCC
BamHI 1315 GGATCC G^GATCC
2014 GGATCC
BfrI 2849 CTTAAG C^TTAAG
3780 CTTAAG
BsaAI 969 TACGTG [CT]AC^GT[GA]
3765 CACGTA
BseRI 380 GAGGAG GAGGAG(10/8)
3184 GAGGAG
BsgI 371 CTGCAC GTGCAG(16/14)
1226 CTGCAC
BsrDI 635 GCAATG GCAATG(2/0)
870 GCAATG
BstUI 9 CGCG CG^CG
112 CGCG
DraII 1143 AGGGCCC [GA]G^G[ACGT]CC[CT]
1144 GGGCCCT
EcoO109I 1143 AGGGCCC [GA]G^G[ACGT]CC[CT]
1144 GGGCCCT
EcoT14I 1683 CCATGG C^C[AT][AT]GG
4052 CCAAGG
FauI 119 CCCGC CCCGC(4/6)
10 GCGGG
HindIII 2192 AAGCTT A^AGCTT
2732 AAGCTT
MslI 1417 CATTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2177 CATTACTATG
MvnI 9 CGCG CG^CG
112 CGCG
PleI 135 GAGTC GAGTC(4/5)
2804 GAGTC
PshBI 1259 ATTAAT AT^TAAT
3862 ATTAAT
PstI 516 CTGCAG CTGCA^G
1431 CTGCAG
SpeI 2657 ACTAGT A^CTAGT
3008 ACTAGT
StyI 1683 CCATGG C^C[AT][AT]GG
4052 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI BanII
BbsI BbvI BfaI BglI BglII
BmyI BpmI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspHI BspLU11I BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EcoRII Fnu4HI FokI HaeIII HincII
HindII HinfI HpaI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI