##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccIII AcsI AflII
AgeI AocI ApoI AscI AseI
AsnI Asp718I AspI AviII AvrII
BamHI BbsI BciVI BclI BfrI
BglII BlnI BpuAI BsaBI BseAI
BsiEI BsiWI BsmI BspEI BspHI
BstBI Bsu36I DrdI EagI EclXI
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FspI HindIII HpaI KpnI
MamI MfeI MluI MroI MunI
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PshAI
PshBI PvuI RcaI SalI SfiI
SfuI SgrAI SnaBI SpeI SspI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AhdI 515 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3105 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 2979 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 515 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BglI 599 GCCGGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 2822 ACCGGA [AT]^CCGG[AT]
BsmBI 2590 GAGACG CGTCTC(1/5)
Bsp1407I 49 TGTACA T^GTACA
BspDI 368 ATCGAT AT^CGAT
BspLU11I 4302 ACATGT A^CATGT
BsrGI 49 TGTACA T^GTACA
BssSI 3603 CTCGTG CACGAG(-5/-1)
ClaI 368 ATCGAT AT^CGAT
CpoI 610 CGGTCCG CG^G[AT]CCG
DraI 1252 TTTAAA TTT^AAA
DraIII 3864 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 515 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3602 CCTCGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 61 GTCAAC GT[CT]^[GA]AC
HindII 61 GTCAAC GT[CT]^[GA]AC
NdeI 2330 CATATG CA^TATG
RsrII 610 CGGTCCG CG^G[AT]CCG
SacI 2516 GAGCTC GAGCT^C
SapI 2719 GAAGAGC GCTCTTC(1/4)
ScaI 80 AGTACT AGT^ACT
SexAI 3865 ACCTGGT A^CC[AT]GGT
SspBI 49 TGTACA T^GTACA
XmnI 2979 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 4018 ACACGT A^C[GA][CT]GT
4302 ACATGT
Alw44I 2962 GTGCAC G^TGCAC
3870 GTGCAC
ApaLI 2962 GTGCAC G^TGCAC
3870 GTGCAC
BbeI 275 GGCGCC GGCGC^C
323 GGCGCC
BcgI 1666 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2056 CGATTCTCCTGC
BsrDI 1617 GCAATG GCAATG(2/0)
2007 GCAATG
Bst1107I 995 GTATAC GTA^TAC
1185 GTATAC
BstAPI 2534 GCAGTTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3104 GCAGCGCCTGC
BstEII 551 GGTAACC G^GT[ACGT]ACC
657 GGTTACC
BstPI 551 GGTAACC G^GT[ACGT]ACC
657 GGTTACC
BstYI 2888 AGATCC [GA]^GATC[CT]
3429 GGATCT
BstZ17I 995 GTATAC GTA^TAC
1185 GTATAC
EarI 1495 CTCTTC CTCTTC(1/4)
2719 GAAGAG
EcoO65I 551 GGTAACC G^GT[ACGT]ACC
657 GGTTACC
FseI 98 GGCCGGCC GGCCGG^CC
4045 GGCCGGCC
KasI 275 GGCGCC G^GCGCC
323 GGCGCC
Ksp632I 1495 CTCTTC CTCTTC(1/4)
2719 GAAGAG
MflI 2888 AGATCC [GA]^GATC[CT]
3429 GGATCT
NaeI 99 GCCGGC GCC^GGC
4046 GCCGGC
NarI 275 GGCGCC GG^CGCC
323 GGCGCC
NgoMIV 99 GCCGGC G^CCGGC
4046 GCCGGC
PaeR7I 1686 CTCGAG C^TCGAG
2076 CTCGAG
SbfI 308 CCTGCAGG CCTGCA^GG
3573 CCTGCAGG
SfoI 275 GGCGCC GGC^GCC
323 GGCGCC
SmlI 1686 CTCGAG C^T[CT][GA]AG
2076 CTCGAG
SphI 3649 GCATGC GCATG^C
4229 GCATGC
Sse8387I 308 CCTGCAGG CCTGCA^GG
3573 CCTGCAGG
Tsp509I 950 AATT ^AATT
1268 AATT
XhoI 1686 CTCGAG C^TCGAG
2076 CTCGAG
XhoII 2888 AGATCC [GA]^GATC[CT]
3429 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccI AccII AciI AclI AcyI
AfaI AluI AlwI Aor51HI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbrPI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI BstXI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eco47III EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KspI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI Psp1406I PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XmaCI XmaI