################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII AclI AflIII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AvaI AviII BalI BamHI BanII BbeI BbrPI BcgI BlpI Bpu1102I BsaAI BsaBI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspLU11I BspMI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraII DrdI EaeI EagI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI KasI KspI MamI MluI MluNI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 1272 GGTACC G^GTACC AccI 1567 GTCTAC GT^[AC][GT]AC AccII 644 CGCG CG^CG AccIII 1045 TCCGGA T^CCGGA AcyI 2198 GACGCC G[GA]^CG[CT]C AhdI 1960 GACTGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1376 CCTCAGG CC^T[ACGT]AGG Asp700I 1188 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1272 GGTACC G^GTACC AspEI 1960 GACTGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 247 GAGCAC G[AT]GC[AT]^C AspI 1224 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 1434 GCTGAGG CCTCAGC(-5/-2) BcnI 95 CCCGG CC^[GC]GG BglI 3632 GCCTTTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3722 AGATCT A^GATCT BsaHI 2198 GACGCC G[GA]^CG[CT]C BsaI 3161 GAGACC GGTCTC(1/5) BsaWI 1045 TCCGGA [AT]^CCGG[AT] BseAI 1045 TCCGGA T^CCGGA BseRI 3032 CTCCTC GAGGAG(10/8) BsiHKAI 247 GAGCAC G[AT]GC[AT]^C BspEI 1045 TCCGGA T^CCGGA BspHI 1196 TCATGA T^CATGA BsrBI 1277 CCGCTC CCGCTC(-3/-3) BsrFI 732 ACCGGC [GA]^CCGG[CT] BstAPI 839 GCAATTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 644 CGCG CG^CG BstXI 946 CCAGTCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1376 CCTCAGG CC^T[ACGT]AGG Cfr10I 732 ACCGGC [GA]^CCGG[CT] Eam1105I 1960 GACTGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1376 CCTCAGG CC^T[ACGT]AGG HaeII 1085 GGCGCT [GA]GCGC^[CT] HgaI 2198 GACGC GACGC(5/10) Hin1I 2198 GACGCC G[GA]^CG[CT]C HindIII 2975 AAGCTT A^AGCTT HpaI 3196 GTTAAC GTT^AAC KpnI 1272 GGTACC GGTAC^C MaeII 3760 ACGT A^CGT MroI 1045 TCCGGA T^CCGGA MvnI 644 CGCG CG^CG NciI 95 CCCGG CC^[GC]GG NcoI 1755 CCATGG C^CATGG PflFI 1224 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 18 CTGCAG CTGCA^G RcaI 1196 TCATGA T^CATGA StuI 1942 AGGCCT AGG^CCT SwaI 2769 ATTTAAAT ATTT^AAAT Tth111I 1224 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1012 CCAAATGCTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1188 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AflII 635 CTTAAG C^TTAAG 2787 CTTAAG AlwNI 1656 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG 1986 CAGCTGCTG AvrII 3165 CCTAGG C^CTAGG 3799 CCTAGG BbsI 3123 GAAGAC GAAGAC(2/6) 3077 GTCTTC BciVI 1136 GGATAC GTATCC(6/5) 2279 GTATCC BclI 364 TGATCA T^GATCA 2446 TGATCA BfrI 635 CTTAAG C^TTAAG 2787 CTTAAG BlnI 3165 CCTAGG C^CTAGG 3799 CCTAGG BmyI 92 GTGCCC G[AGT]GC[ACT]^C 247 GAGCAC BpuAI 3123 GAAGAC GAAGAC(2/6) 3077 GTCTTC BsgI 1694 GTGCAG GTGCAG(16/14) 2072 GTGCAG BsmFI 428 GGGAC GGGAC(10/14) 1968 GTCCC Bsp1286I 92 GTGCCC G[AGT]GC[ACT]^C 247 GAGCAC CfoI 1 GCGC GCG^C 1086 GCGC DraIII 1234 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG 3494 CACTTAGTG DsaI 34 CCGTGG C^C[GA][CT]GG 1755 CCATGG FauI 1276 CCCGC CCCGC(4/6) 1719 CCCGC FbaI 364 TGATCA T^GATCA 2446 TGATCA HhaI 1 GCGC GCG^C 1086 GCGC HinP1I 1 GCGC G^CGC 1086 GCGC NspI 768 GCATGT [GA]CATG^[CT] 1699 GCATGT TaqI 2157 TCGA T^CGA 2643 TCGA TthHB8I 2157 TCGA T^CGA 2643 TCGA XbaI 232 TCTAGA T^CTAGA 3090 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaII BanI BbvI BfaI BpmI BsaJI BsiYI BslI BsmAI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MspI MunI MvaI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII