##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII AclI AflIII AgeI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI AvaI AviII BalI BamHI
BanII BbeI BbrPI BcgI BlpI
Bpu1102I BsaAI BsaBI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspLU11I BspMI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraII DrdI
EaeI EagI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI KasI KspI
MamI MluI MluNI MscI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI Van91I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 1272 GGTACC G^GTACC
AccI 1567 GTCTAC GT^[AC][GT]AC
AccII 644 CGCG CG^CG
AccIII 1045 TCCGGA T^CCGGA
AcyI 2198 GACGCC G[GA]^CG[CT]C
AhdI 1960 GACTGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1376 CCTCAGG CC^T[ACGT]AGG
Asp700I 1188 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1272 GGTACC G^GTACC
AspEI 1960 GACTGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 247 GAGCAC G[AT]GC[AT]^C
AspI 1224 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1434 GCTGAGG CCTCAGC(-5/-2)
BcnI 95 CCCGG CC^[GC]GG
BglI 3632 GCCTTTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3722 AGATCT A^GATCT
BsaHI 2198 GACGCC G[GA]^CG[CT]C
BsaI 3161 GAGACC GGTCTC(1/5)
BsaWI 1045 TCCGGA [AT]^CCGG[AT]
BseAI 1045 TCCGGA T^CCGGA
BseRI 3032 CTCCTC GAGGAG(10/8)
BsiHKAI 247 GAGCAC G[AT]GC[AT]^C
BspEI 1045 TCCGGA T^CCGGA
BspHI 1196 TCATGA T^CATGA
BsrBI 1277 CCGCTC CCGCTC(-3/-3)
BsrFI 732 ACCGGC [GA]^CCGG[CT]
BstAPI 839 GCAATTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 644 CGCG CG^CG
BstXI 946 CCAGTCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1376 CCTCAGG CC^T[ACGT]AGG
Cfr10I 732 ACCGGC [GA]^CCGG[CT]
Eam1105I 1960 GACTGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1376 CCTCAGG CC^T[ACGT]AGG
HaeII 1085 GGCGCT [GA]GCGC^[CT]
HgaI 2198 GACGC GACGC(5/10)
Hin1I 2198 GACGCC G[GA]^CG[CT]C
HindIII 2975 AAGCTT A^AGCTT
HpaI 3196 GTTAAC GTT^AAC
KpnI 1272 GGTACC GGTAC^C
MaeII 3760 ACGT A^CGT
MroI 1045 TCCGGA T^CCGGA
MvnI 644 CGCG CG^CG
NciI 95 CCCGG CC^[GC]GG
NcoI 1755 CCATGG C^CATGG
PflFI 1224 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 18 CTGCAG CTGCA^G
RcaI 1196 TCATGA T^CATGA
StuI 1942 AGGCCT AGG^CCT
SwaI 2769 ATTTAAAT ATTT^AAAT
Tth111I 1224 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1012 CCAAATGCTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1188 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflII 635 CTTAAG C^TTAAG
2787 CTTAAG
AlwNI 1656 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
1986 CAGCTGCTG
AvrII 3165 CCTAGG C^CTAGG
3799 CCTAGG
BbsI 3123 GAAGAC GAAGAC(2/6)
3077 GTCTTC
BciVI 1136 GGATAC GTATCC(6/5)
2279 GTATCC
BclI 364 TGATCA T^GATCA
2446 TGATCA
BfrI 635 CTTAAG C^TTAAG
2787 CTTAAG
BlnI 3165 CCTAGG C^CTAGG
3799 CCTAGG
BmyI 92 GTGCCC G[AGT]GC[ACT]^C
247 GAGCAC
BpuAI 3123 GAAGAC GAAGAC(2/6)
3077 GTCTTC
BsgI 1694 GTGCAG GTGCAG(16/14)
2072 GTGCAG
BsmFI 428 GGGAC GGGAC(10/14)
1968 GTCCC
Bsp1286I 92 GTGCCC G[AGT]GC[ACT]^C
247 GAGCAC
CfoI 1 GCGC GCG^C
1086 GCGC
DraIII 1234 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3494 CACTTAGTG
DsaI 34 CCGTGG C^C[GA][CT]GG
1755 CCATGG
FauI 1276 CCCGC CCCGC(4/6)
1719 CCCGC
FbaI 364 TGATCA T^GATCA
2446 TGATCA
HhaI 1 GCGC GCG^C
1086 GCGC
HinP1I 1 GCGC G^CGC
1086 GCGC
NspI 768 GCATGT [GA]CATG^[CT]
1699 GCATGT
TaqI 2157 TCGA T^CGA
2643 TCGA
TthHB8I 2157 TCGA T^CGA
2643 TCGA
XbaI 232 TCTAGA T^CTAGA
3090 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BbvI BfaI
BpmI BsaJI BsiYI BslI BsmAI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MfeI
MflI MnlI MseI MslI MspA1I
MspI MunI MvaI MwoI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII