################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## AatII AclI AgeI Aor51HI AscI AseI AsnI Asp700I AviII AvrII BbeI BbrPI BciVI BglII BlnI BsiEI BsiWI Bsp1407I BspDI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoRV EcoT22I FseI FspI HaeII HincII HindII HindIII HpaI KasI KspI MfeI MluI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccI 4042 GTAGAC GT^[AC][GT]AC AccII 2777 CGCG CG^CG AflIII 2119 ACATGT A^C[GA][CT]GT Alw44I 3188 GTGCAC G^TGCAC ApaLI 3188 GTGCAC G^TGCAC AspI 195 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 2941 CTCGGG C^[CT]CG[GA]G BcgI 2955 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1663 TGATCA T^GATCA BsaAI 649 CACGTA [CT]AC^GT[GA] BsaBI 1324 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsoBI 2941 CTCGGG C^[CT]CG[GA]G BspHI 4351 TCATGA T^CATGA BspLU11I 2119 ACATGT A^CATGT BsrFI 4022 ACCGGC [GA]^CCGG[CT] BssSI 591 CACGAG CACGAG(-5/-1) BstEII 117 GGTCACC G^GT[ACGT]ACC BstPI 117 GGTCACC G^GT[ACGT]ACC BstUI 2777 CGCG CG^CG Cfr10I 4022 ACCGGC [GA]^CCGG[CT] EcoO65I 117 GGTCACC G^GT[ACGT]ACC EcoRI 4255 GAATTC G^AATTC FbaI 1663 TGATCA T^GATCA MamI 1324 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 2777 CGCG CG^CG NdeI 2766 CATATG CA^TATG PflFI 195 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 540 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 2459 CTGCAG CTGCA^G PvuII 3225 CAGCTG CAG^CTG RcaI 4351 TCATGA T^CATGA SexAI 4037 ACCTGGT A^CC[AT]GGT SspI 697 AATATT AAT^ATT StuI 3718 AGGCCT AGG^CCT Tth111I 195 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccIII 372 TCCGGA T^CCGGA 386 TCCGGA AcyI 636 GGCGTC G[GA]^CG[CT]C 4045 GACGCC AflII 91 CTTAAG C^TTAAG 2740 CTTAAG AhdI 2590 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2728 GACTAGGGGTC AlwNI 762 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG 3306 CAGTGCCTG AocI 20 CCTTAGG CC^T[ACGT]AGG 2833 CCTGAGG AspEI 2590 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2728 GACTAGGGGTC BamHI 1201 GGATCC G^GATCC 1908 GGATCC BfrI 91 CTTAAG C^TTAAG 2740 CTTAAG BlpI 530 GCTCAGC GC^T[ACGT]AGC 4110 GCTGAGC Bpu1102I 530 GCTCAGC GC^T[ACGT]AGC 4110 GCTGAGC BsaHI 636 GGCGTC G[GA]^CG[CT]C 4045 GACGCC BseAI 372 TCCGGA T^CCGGA 386 TCCGGA BsmBI 2880 CGTCTC CGTCTC(1/5) 3044 CGTCTC BspEI 372 TCCGGA T^CCGGA 386 TCCGGA BsrBI 1671 CCGCTC CCGCTC(-3/-3) 438 GAGCGG BsrDI 3010 CATTGC GCAATG(2/0) 3327 GCAATG BstXI 863 CCACTTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2800 CCAGCACTTTGG Bsu36I 20 CCTTAGG CC^T[ACGT]AGG 2833 CCTGAGG CelII 530 GCTCAGC GC^T[ACGT]AGC 4110 GCTGAGC Eam1105I 2590 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2728 GACTAGGGGTC Eco81I 20 CCTTAGG CC^T[ACGT]AGG 2833 CCTGAGG EcoNI 509 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3311 CCTGATACAGG Hin1I 636 GGCGTC G[GA]^CG[CT]C 4045 GACGCC MaeII 650 ACGT A^CGT 3553 ACGT MroI 372 TCCGGA T^CCGGA 386 TCCGGA MspA1I 1002 CCGCTG C[AC]G^C[GT]G 3225 CAGCTG PflMI 1140 CCAATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1912 CCACCCCCTGG SacI 528 GAGCTC GAGCT^C 3617 GAGCTC TaqI 2848 TCGA T^CGA 4277 TCGA TthHB8I 2848 TCGA T^CGA 4277 TCGA Van91I 1140 CCAATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1912 CCACCCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### Acc65I AciI AcsI AfaI AluI AlwI ApaI ApoI Asp718I AspHI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII