##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AclI AgeI Aor51HI AscI
AseI AsnI Asp700I AviII AvrII
BbeI BbrPI BciVI BglII BlnI
BsiEI BsiWI Bsp1407I BspDI BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoRV EcoT22I FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KspI MfeI MluI MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 4042 GTAGAC GT^[AC][GT]AC
AccII 2777 CGCG CG^CG
AflIII 2119 ACATGT A^C[GA][CT]GT
Alw44I 3188 GTGCAC G^TGCAC
ApaLI 3188 GTGCAC G^TGCAC
AspI 195 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2941 CTCGGG C^[CT]CG[GA]G
BcgI 2955 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1663 TGATCA T^GATCA
BsaAI 649 CACGTA [CT]AC^GT[GA]
BsaBI 1324 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 2941 CTCGGG C^[CT]CG[GA]G
BspHI 4351 TCATGA T^CATGA
BspLU11I 2119 ACATGT A^CATGT
BsrFI 4022 ACCGGC [GA]^CCGG[CT]
BssSI 591 CACGAG CACGAG(-5/-1)
BstEII 117 GGTCACC G^GT[ACGT]ACC
BstPI 117 GGTCACC G^GT[ACGT]ACC
BstUI 2777 CGCG CG^CG
Cfr10I 4022 ACCGGC [GA]^CCGG[CT]
EcoO65I 117 GGTCACC G^GT[ACGT]ACC
EcoRI 4255 GAATTC G^AATTC
FbaI 1663 TGATCA T^GATCA
MamI 1324 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2777 CGCG CG^CG
NdeI 2766 CATATG CA^TATG
PflFI 195 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 540 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2459 CTGCAG CTGCA^G
PvuII 3225 CAGCTG CAG^CTG
RcaI 4351 TCATGA T^CATGA
SexAI 4037 ACCTGGT A^CC[AT]GGT
SspI 697 AATATT AAT^ATT
StuI 3718 AGGCCT AGG^CCT
Tth111I 195 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 372 TCCGGA T^CCGGA
386 TCCGGA
AcyI 636 GGCGTC G[GA]^CG[CT]C
4045 GACGCC
AflII 91 CTTAAG C^TTAAG
2740 CTTAAG
AhdI 2590 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2728 GACTAGGGGTC
AlwNI 762 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
3306 CAGTGCCTG
AocI 20 CCTTAGG CC^T[ACGT]AGG
2833 CCTGAGG
AspEI 2590 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2728 GACTAGGGGTC
BamHI 1201 GGATCC G^GATCC
1908 GGATCC
BfrI 91 CTTAAG C^TTAAG
2740 CTTAAG
BlpI 530 GCTCAGC GC^T[ACGT]AGC
4110 GCTGAGC
Bpu1102I 530 GCTCAGC GC^T[ACGT]AGC
4110 GCTGAGC
BsaHI 636 GGCGTC G[GA]^CG[CT]C
4045 GACGCC
BseAI 372 TCCGGA T^CCGGA
386 TCCGGA
BsmBI 2880 CGTCTC CGTCTC(1/5)
3044 CGTCTC
BspEI 372 TCCGGA T^CCGGA
386 TCCGGA
BsrBI 1671 CCGCTC CCGCTC(-3/-3)
438 GAGCGG
BsrDI 3010 CATTGC GCAATG(2/0)
3327 GCAATG
BstXI 863 CCACTTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2800 CCAGCACTTTGG
Bsu36I 20 CCTTAGG CC^T[ACGT]AGG
2833 CCTGAGG
CelII 530 GCTCAGC GC^T[ACGT]AGC
4110 GCTGAGC
Eam1105I 2590 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2728 GACTAGGGGTC
Eco81I 20 CCTTAGG CC^T[ACGT]AGG
2833 CCTGAGG
EcoNI 509 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3311 CCTGATACAGG
Hin1I 636 GGCGTC G[GA]^CG[CT]C
4045 GACGCC
MaeII 650 ACGT A^CGT
3553 ACGT
MroI 372 TCCGGA T^CCGGA
386 TCCGGA
MspA1I 1002 CCGCTG C[AC]G^C[GT]G
3225 CAGCTG
PflMI 1140 CCAATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1912 CCACCCCCTGG
SacI 528 GAGCTC GAGCT^C
3617 GAGCTC
TaqI 2848 TCGA T^CGA
4277 TCGA
TthHB8I 2848 TCGA T^CGA
4277 TCGA
Van91I 1140 CCAATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1912 CCACCCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AluI
AlwI ApaI ApoI Asp718I AspHI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII