##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
AocI AscI AseI AsnI Asp700I
AspEI AvrII BamHI BciVI BlnI
BsaWI BseAI BspEI BssSI Bst1107I
BstEII BstPI BstZ17I Bsu36I CpoI
DraI Eam1105I Eco81I EcoO65I EcoRV
EcoT22I FseI HpaI MfeI MroI
MunI NdeI NheI NotI NruI
NsiI PacI PinAI PmeI PshBI
Psp1406I PvuI RsrII SapI ScaI
SfiI SgrAI SnaBI SpeI SspI
SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 2195 GGTACC G^GTACC
Asp718I 2195 GGTACC G^GTACC
AspI 1186 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2000 TGCGCA TGC^GCA
BbeI 859 GGCGCC GGCGC^C
BbrPI 2850 CACGTG CAC^GTG
BbsI 2145 GAAGAC GAAGAC(2/6)
BcgI 97 CGAGAACCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2372 TGATCA T^GATCA
BglII 3594 AGATCT A^GATCT
BpuAI 2145 GAAGAC GAAGAC(2/6)
BsaBI 2541 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 1575 CGTACG C^GTACG
BsmBI 3611 CGTCTC CGTCTC(1/5)
BspDI 1783 ATCGAT AT^CGAT
BspHI 2480 TCATGA T^CATGA
BspLU11I 3861 ACATGT A^CATGT
BsrDI 3538 CATTGC GCAATG(2/0)
BstBI 1380 TTCGAA TT^CGAA
BstYI 3594 AGATCT [GA]^GATC[CT]
ClaI 1783 ATCGAT AT^CGAT
DraIII 2725 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3633 GACCCCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 675 GAATTC G^AATTC
FbaI 2372 TGATCA T^GATCA
FspI 2000 TGCGCA TGC^GCA
HindIII 2293 AAGCTT A^AGCTT
KasI 859 GGCGCC G^GCGCC
KpnI 2195 GGTACC GGTAC^C
MamI 2541 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 3594 AGATCT [GA]^GATC[CT]
NarI 859 GGCGCC GG^CGCC
NspV 1380 TTCGAA TT^CGAA
PaeR7I 901 CTCGAG C^TCGAG
PflFI 1186 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2850 CACGTG CAC^GTG
PmlI 2850 CACGTG CAC^GTG
PpuMI 3809 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 2480 TCATGA T^CATGA
SalI 117 GTCGAC G^TCGAC
SbfI 293 CCTGCAGG CCTGCA^GG
SexAI 2204 ACCAGGT A^CC[AT]GGT
SfoI 859 GGCGCC GGC^GCC
SfuI 1380 TTCGAA TT^CGAA
Sse8387I 293 CCTGCAGG CCTGCA^GG
Tth111I 1186 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 901 CTCGAG C^TCGAG
XhoII 3594 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 117 GTCGAC GT^[AC][GT]AC
3079 GTCTAC
AcsI 675 GAATTC [GA]^AATT[CT]
3868 AAATTC
AcyI 859 GGCGCC G[GA]^CG[CT]C
3702 GGCGTC
AflII 763 CTTAAG C^TTAAG
1894 CTTAAG
Alw44I 1716 GTGCAC G^TGCAC
2853 GTGCAC
ApaLI 1716 GTGCAC G^TGCAC
2853 GTGCAC
ApoI 675 GAATTC [GA]^AATT[CT]
3868 AAATTC
BfrI 763 CTTAAG C^TTAAG
1894 CTTAAG
BsaHI 859 GGCGCC G[GA]^CG[CT]C
3702 GGCGTC
Bsp1407I 2927 TGTACA T^GTACA
2999 TGTACA
BsrGI 2927 TGTACA T^GTACA
2999 TGTACA
BssHII 41 GCGCGC G^CGCGC
186 GCGCGC
BstXI 1040 CCATCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3402 CCACGGACGTGG
Hin1I 859 GGCGCC G[GA]^CG[CT]C
3702 GGCGTC
HincII 117 GTCGAC GT[CT]^[GA]AC
1912 GTCAAC
HindII 117 GTCGAC GT[CT]^[GA]AC
1912 GTCAAC
MluI 3876 ACGCGT A^CGCGT
3888 ACGCGT
NaeI 143 GCCGGC GCC^GGC
2273 GCCGGC
NgoMIV 143 GCCGGC G^CCGGC
2273 GCCGGC
PshAI 1831 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3261 GACTTACGTC
SacI 1029 GAGCTC GAGCT^C
3217 GAGCTC
SmaI 79 CCCGGG CCC^GGG
133 CCCGGG
SphI 2159 GCATGC GCATG^C
2834 GCATGC
SspBI 2927 TGTACA T^GTACA
2999 TGTACA
XmaCI 79 CCCGGG C^CCGGG
133 CCCGGG
XmaI 79 CCCGGG C^CCGGG
133 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI Aor51HI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI