################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## Acc65I AccI AccIII ApaI AscI Asp718I AspI AvrII BbeI BbrPI BcgI BlnI BseAI BsiEI BsiWI BspEI BssHII Bst1107I BstBI BstZ17I CpoI DraIII DrdI EagI EclXI Eco52I FseI KasI KpnI KspI MluI MroI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PflFI PmaCI PmeI PmlI PshAI PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SwaI Tth111I ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 5942 GACGTC GACGT^C AccII 2992 CGCG CG^CG AcyI 5942 GACGTC G[GA]^CG[CT]C AflII 3655 CTTAAG C^TTAAG AgeI 5984 ACCGGT A^CCGGT AhdI 3346 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 911 GTGCAC G^TGCAC Aor51HI 2225 AGCGCT AGC^GCT ApaLI 911 GTGCAC G^TGCAC AspEI 3346 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2243 TGCGCA TGC^GCA BbvCI 6764 CCTCAGC CCTCAGC(-5/-2) BciVI 1193 GTATCC GTATCC(6/5) BclI 4124 TGATCA T^GATCA BfrI 3655 CTTAAG C^TTAAG BlpI 6741 GCTGAGC GC^T[ACGT]AGC Bpu1102I 6741 GCTGAGC GC^T[ACGT]AGC BsaHI 5942 GACGTC G[GA]^CG[CT]C BsaWI 5984 ACCGGT [AT]^CCGG[AT] BseRI 1810 GAGGAG GAGGAG(10/8) BsmBI 1946 GAGACG CGTCTC(1/5) Bsp1407I 1742 TGTACA T^GTACA BsrBI 6113 CCGCTC CCGCTC(-3/-3) BsrFI 5984 ACCGGT [GA]^CCGG[CT] BsrGI 1742 TGTACA T^GTACA BstUI 2992 CGCG CG^CG CelII 6741 GCTGAGC GC^T[ACGT]AGC Cfr10I 5984 ACCGGT [GA]^CCGG[CT] Eam1105I 3346 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2225 AGCGCT AGC^GCT EcoNI 5242 CCTCCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 3615 GAATTC G^AATTC EcoRV 3456 GATATC GAT^ATC FbaI 4124 TGATCA T^GATCA FspI 2243 TGCGCA TGC^GCA Hin1I 5942 GACGTC G[GA]^CG[CT]C HpaI 202 GTTAAC GTT^AAC MfeI 1358 CAATTG C^AATTG MunI 1358 CAATTG C^AATTG MvnI 2992 CGCG CG^CG NdeI 391 CATATG CA^TATG PinAI 5984 ACCGGT A^CCGGT SacI 5113 GAGCTC GAGCT^C ScaI 4223 AGTACT AGT^ACT SexAI 6104 ACCAGGT A^CC[AT]GGT SmaI 6623 CCCGGG CCC^GGG SpeI 2388 ACTAGT A^CTAGT SspBI 1742 TGTACA T^GTACA XbaI 3678 TCTAGA T^CTAGA XmaCI 6623 CCCGGG C^CCGGG XmaI 6623 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AclI 74 AACGTT AA^CGTT 2194 AACGTT AseI 1288 ATTAAT AT^TAAT 2857 ATTAAT AsnI 1288 ATTAAT AT^TAAT 2857 ATTAAT BanI 4078 GGTGCC G^G[CT][GA]CC 6757 GGTGCC BglI 2742 GCCTGATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2995 GCCGCACAGGC BsaAI 3161 TACGTG [CT]AC^GT[GA] 5255 TACGTG BsaI 5764 GAGACC GGTCTC(1/5) 2359 GGTCTC BspDI 3370 ATCGAT AT^CGAT 5704 ATCGAT BspHI 1722 TCATGA T^CATGA 2699 TCATGA BspLU11I 1557 ACATGT A^CATGT 4880 ACATGT BstEII 5229 GGTGACC G^GT[ACGT]ACC 6669 GGTAACC BstPI 5229 GGTGACC G^GT[ACGT]ACC 6669 GGTAACC ClaI 3370 ATCGAT AT^CGAT 5704 ATCGAT DraII 5010 AGGACCT [GA]G^G[ACGT]CC[CT] 6184 AGGACCT EcoO109I 5010 AGGACCT [GA]G^G[ACGT]CC[CT] 6184 AGGACCT EcoO65I 5229 GGTGACC G^GT[ACGT]ACC 6669 GGTAACC HaeII 2225 AGCGCT [GA]GCGC^[CT] 4298 AGCGCC HgaI 1980 GCGTC GACGC(5/10) 4413 GCGTC HincII 202 GTTAAC GT[CT]^[GA]AC 1120 GTTGAC HindII 202 GTTAAC GT[CT]^[GA]AC 1120 GTTGAC PaeR7I 2951 CTCGAG C^TCGAG 3610 CTCGAG PflMI 919 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2075 CCATTGACTGG PpuMI 5010 AGGACCT [GA]G^G[AT]CC[CT] 6184 AGGACCT PshBI 1288 ATTAAT AT^TAAT 2857 ATTAAT Psp1406I 74 AACGTT AA^CGTT 2194 AACGTT PstI 4466 CTGCAG CTGCA^G 6365 CTGCAG RcaI 1722 TCATGA T^CATGA 2699 TCATGA SapI 3649 GAAGAGC GCTCTTC(1/4) 5110 GAAGAGC SphI 968 GCATGC GCATG^C 4534 GCATGC Van91I 919 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2075 CCATTGACTGG XhoI 2951 CTCGAG C^TCGAG 3610 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaI AvaII BalI BamHI BanII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaBI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BssSI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI EaeI EarI Eco81I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI