##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
Acc65I AccI AccIII ApaI AscI
Asp718I AspI AvrII BbeI BbrPI
BcgI BlnI BseAI BsiEI BsiWI
BspEI BssHII Bst1107I BstBI BstZ17I
CpoI DraIII DrdI EagI EclXI
Eco52I FseI KasI KpnI KspI
MluI MroI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PflFI PmaCI PmeI PmlI PshAI
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SnaBI
Sse8387I SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 5942 GACGTC GACGT^C
AccII 2992 CGCG CG^CG
AcyI 5942 GACGTC G[GA]^CG[CT]C
AflII 3655 CTTAAG C^TTAAG
AgeI 5984 ACCGGT A^CCGGT
AhdI 3346 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 911 GTGCAC G^TGCAC
Aor51HI 2225 AGCGCT AGC^GCT
ApaLI 911 GTGCAC G^TGCAC
AspEI 3346 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2243 TGCGCA TGC^GCA
BbvCI 6764 CCTCAGC CCTCAGC(-5/-2)
BciVI 1193 GTATCC GTATCC(6/5)
BclI 4124 TGATCA T^GATCA
BfrI 3655 CTTAAG C^TTAAG
BlpI 6741 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 6741 GCTGAGC GC^T[ACGT]AGC
BsaHI 5942 GACGTC G[GA]^CG[CT]C
BsaWI 5984 ACCGGT [AT]^CCGG[AT]
BseRI 1810 GAGGAG GAGGAG(10/8)
BsmBI 1946 GAGACG CGTCTC(1/5)
Bsp1407I 1742 TGTACA T^GTACA
BsrBI 6113 CCGCTC CCGCTC(-3/-3)
BsrFI 5984 ACCGGT [GA]^CCGG[CT]
BsrGI 1742 TGTACA T^GTACA
BstUI 2992 CGCG CG^CG
CelII 6741 GCTGAGC GC^T[ACGT]AGC
Cfr10I 5984 ACCGGT [GA]^CCGG[CT]
Eam1105I 3346 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2225 AGCGCT AGC^GCT
EcoNI 5242 CCTCCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3615 GAATTC G^AATTC
EcoRV 3456 GATATC GAT^ATC
FbaI 4124 TGATCA T^GATCA
FspI 2243 TGCGCA TGC^GCA
Hin1I 5942 GACGTC G[GA]^CG[CT]C
HpaI 202 GTTAAC GTT^AAC
MfeI 1358 CAATTG C^AATTG
MunI 1358 CAATTG C^AATTG
MvnI 2992 CGCG CG^CG
NdeI 391 CATATG CA^TATG
PinAI 5984 ACCGGT A^CCGGT
SacI 5113 GAGCTC GAGCT^C
ScaI 4223 AGTACT AGT^ACT
SexAI 6104 ACCAGGT A^CC[AT]GGT
SmaI 6623 CCCGGG CCC^GGG
SpeI 2388 ACTAGT A^CTAGT
SspBI 1742 TGTACA T^GTACA
XbaI 3678 TCTAGA T^CTAGA
XmaCI 6623 CCCGGG C^CCGGG
XmaI 6623 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AclI 74 AACGTT AA^CGTT
2194 AACGTT
AseI 1288 ATTAAT AT^TAAT
2857 ATTAAT
AsnI 1288 ATTAAT AT^TAAT
2857 ATTAAT
BanI 4078 GGTGCC G^G[CT][GA]CC
6757 GGTGCC
BglI 2742 GCCTGATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2995 GCCGCACAGGC
BsaAI 3161 TACGTG [CT]AC^GT[GA]
5255 TACGTG
BsaI 5764 GAGACC GGTCTC(1/5)
2359 GGTCTC
BspDI 3370 ATCGAT AT^CGAT
5704 ATCGAT
BspHI 1722 TCATGA T^CATGA
2699 TCATGA
BspLU11I 1557 ACATGT A^CATGT
4880 ACATGT
BstEII 5229 GGTGACC G^GT[ACGT]ACC
6669 GGTAACC
BstPI 5229 GGTGACC G^GT[ACGT]ACC
6669 GGTAACC
ClaI 3370 ATCGAT AT^CGAT
5704 ATCGAT
DraII 5010 AGGACCT [GA]G^G[ACGT]CC[CT]
6184 AGGACCT
EcoO109I 5010 AGGACCT [GA]G^G[ACGT]CC[CT]
6184 AGGACCT
EcoO65I 5229 GGTGACC G^GT[ACGT]ACC
6669 GGTAACC
HaeII 2225 AGCGCT [GA]GCGC^[CT]
4298 AGCGCC
HgaI 1980 GCGTC GACGC(5/10)
4413 GCGTC
HincII 202 GTTAAC GT[CT]^[GA]AC
1120 GTTGAC
HindII 202 GTTAAC GT[CT]^[GA]AC
1120 GTTGAC
PaeR7I 2951 CTCGAG C^TCGAG
3610 CTCGAG
PflMI 919 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2075 CCATTGACTGG
PpuMI 5010 AGGACCT [GA]G^G[AT]CC[CT]
6184 AGGACCT
PshBI 1288 ATTAAT AT^TAAT
2857 ATTAAT
Psp1406I 74 AACGTT AA^CGTT
2194 AACGTT
PstI 4466 CTGCAG CTGCA^G
6365 CTGCAG
RcaI 1722 TCATGA T^CATGA
2699 TCATGA
SapI 3649 GAAGAGC GCTCTTC(1/4)
5110 GAAGAGC
SphI 968 GCATGC GCATG^C
4534 GCATGC
Van91I 919 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2075 CCATTGACTGG
XhoI 2951 CTCGAG C^TCGAG
3610 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaI AvaII BalI BamHI
BanII BbsI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaBI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
Eco81I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI