##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AspEI AspI AviII AvrII
BbeI BbrPI BcgI BcnI BfrI
BlnI BlpI Bpu1102I BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BspDI
BspEI BspHI BspMI BsrBI BssHII
BssSI BstBI BstXI Bsu36I CelII
CfoI ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoRV
EcoT14I FauI FseI FspI HaeII
HgaI HhaI HinP1I HincII HindII
HindIII HpaI KasI KspI MluI
MroI MspA1I NarI NciI NcoI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SpeI SphI Sse8387I StuI StyI
Tth111I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 652 GACGTC GACGT^C
Acc65I 1149 GGTACC G^GTACC
AccII 1021 CGCG CG^CG
AcyI 652 GACGTC G[GA]^CG[CT]C
AseI 3630 ATTAAT AT^TAAT
AsnI 3630 ATTAAT AT^TAAT
Asp718I 1149 GGTACC G^GTACC
AspHI 2419 GTGCTC G[AT]GC[AT]^C
AvaI 737 CCCGAG C^[CT]CG[GA]G
BalI 5 TGGCCA TGG^CCA
BamHI 1241 GGATCC G^GATCC
BanII 1221 GGGCTC G[GA]GC[CT]^C
BbvCI 1004 CCTCAGC CCTCAGC(-5/-2)
BclI 2785 TGATCA T^GATCA
BglI 2564 GCCATTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 812 AGATCT A^GATCT
BsaAI 1921 TACGTA [CT]AC^GT[GA]
BsaHI 652 GACGTC G[GA]^CG[CT]C
BseRI 943 GAGGAG GAGGAG(10/8)
BsiHKAI 2419 GTGCTC G[AT]GC[AT]^C
BsoBI 737 CCCGAG C^[CT]CG[GA]G
BsrFI 55 GCCGGC [GA]^CCGG[CT]
Bst1107I 3862 GTATAC GTA^TAC
BstAPI 1909 GCATAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2863 GGTCACC G^GT[ACGT]ACC
BstPI 2863 GGTCACC G^GT[ACGT]ACC
BstUI 1021 CGCG CG^CG
BstZ17I 3862 GTATAC GTA^TAC
Cfr10I 55 GCCGGC [GA]^CCGG[CT]
EcoNI 151 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2863 GGTCACC G^GT[ACGT]ACC
EcoT22I 3573 ATGCAT ATGCA^T
FbaI 2785 TGATCA T^GATCA
HapII 56 CCGG C^CGG
Hin1I 652 GACGTC G[GA]^CG[CT]C
HpaII 56 CCGG C^CGG
KpnI 1149 GGTACC GGTAC^C
MluNI 5 TGGCCA TGG^CCA
MscI 5 TGGCCA TGG^CCA
MspI 56 CCGG C^CGG
MvnI 1021 CGCG CG^CG
NaeI 55 GCCGGC GCC^GGC
NgoMIV 55 GCCGGC G^CCGGC
NsiI 3573 ATGCAT ATGCA^T
PflMI 660 CCACGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 3630 ATTAAT AT^TAAT
PstI 1423 CTGCAG CTGCA^G
ScaI 797 AGTACT AGT^ACT
SnaBI 1921 TACGTA TAC^GTA
SwaI 3392 ATTTAAAT ATTT^AAAT
Van91I 660 CCACGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 693 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1291 GTAGAC GT^[AC][GT]AC
3862 GTATAC
AciI 326 CCGC CCGC(-3/-1)
683 GCGG
Asp700I 3206 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3496 GAATGTCTTC
AvaII 1856 GGACC G^G[AT]CC
2649 GGACC
BanI 118 GGCACC G^G[CT][GA]CC
1149 GGTACC
BciVI 2711 GGATAC GTATCC(6/5)
1976 GTATCC
BpmI 2013 CTCCAG CTGGAG(16/14)
1702 CTGGAG
BsaI 415 GAGACC GGTCTC(1/5)
637 GGTCTC
BsmFI 2648 GGGAC GGGAC(10/14)
429 GTCCC
Bsp1407I 2126 TGTACA T^GTACA
3646 TGTACA
BsrGI 2126 TGTACA T^GTACA
3646 TGTACA
Cfr13I 1856 GGACC G^G[ACGT]CC
2649 GGACC
EaeI 5 TGGCCA [CT]^GGCC[GA]
53 TGGCCG
MfeI 560 CAATTG C^AATTG
3344 CAATTG
MslI 420 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
967 CACAGTAGTG
MunI 560 CAATTG C^AATTG
3344 CAATTG
NdeI 824 CATATG CA^TATG
1717 CATATG
Sau96I 1856 GGACC G^G[ACGT]CC
2649 GGACC
SfcI 1289 CTGTAG C^T[GA][CT]AG
1423 CTGCAG
SspBI 2126 TGTACA T^GTACA
3646 TGTACA
TaqI 450 TCGA T^CGA
2842 TCGA
TthHB8I 450 TCGA T^CGA
2842 TCGA
XmnI 3206 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3496 GAATGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
AlwNI ApoI BbsI BbvI BfaI
BmyI BpuAI BsaBI BsaJI BsiYI
BslI BsmAI BsmI Bsp1286I BspLU11I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I DdeI DpnII DraI
EarI EcoRI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI ScrFI SfaNI
SspI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII