##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccI AccIII AclI AflIII
Alw44I Aor51HI ApaLI AscI Asp700I
AviII AvrII BamHI BbrPI BciVI
BglII BlnI BsaBI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI FseI FspI
MamI MflI MluI MroI NaeI
NgoMIV NotI NspV PacI PaeR7I
PmaCI PmeI PmlI Psp1406I PstI
PvuI RsrII SalI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SwaI XhoI XhoII
XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 553 GGTACC G^GTACC
AflII 558 CTTAAG C^TTAAG
AgeI 2799 ACCGGT A^CCGGT
AseI 2992 ATTAAT AT^TAAT
AsnI 2992 ATTAAT AT^TAAT
Asp718I 553 GGTACC G^GTACC
AspI 1864 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 2532 GCAAAGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 64 TGATCA T^GATCA
BfrI 558 CTTAAG C^TTAAG
BsaAI 2849 TACGTG [CT]AC^GT[GA]
BsaWI 2799 ACCGGT [AT]^CCGG[AT]
BssHII 2410 GCGCGC G^CGCGC
BssSI 1653 CACGAG CACGAG(-5/-1)
DraI 520 TTTAAA TTT^AAA
EcoRV 3821 GATATC GAT^ATC
EcoT22I 421 ATGCAT ATGCA^T
FbaI 64 TGATCA T^GATCA
HindIII 2983 AAGCTT A^AGCTT
HpaI 626 GTTAAC GTT^AAC
KpnI 553 GGTACC GGTAC^C
KspI 2963 CCGCGG CCGC^GG
MfeI 2776 CAATTG C^AATTG
MunI 2776 CAATTG C^AATTG
NcoI 3046 CCATGG C^CATGG
NdeI 1274 CATATG CA^TATG
NheI 2289 GCTAGC G^CTAGC
NruI 6 TCGCGA TCG^CGA
NsiI 421 ATGCAT ATGCA^T
NspI 1073 GCATGC [GA]CATG^[CT]
PflFI 1864 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2799 ACCGGT A^CCGGT
PpuMI 3724 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 2992 ATTAAT AT^TAAT
SacI 3090 GAGCTC GAGCT^C
SacII 2963 CCGCGG CCGC^GG
SapI 537 GAAGAGC GCTCTTC(1/4)
SmaI 2872 CCCGGG CCC^GGG
SphI 1073 GCATGC GCATG^C
StuI 2376 AGGCCT AGG^CCT
TaqI 2720 TCGA T^CGA
Tth111I 1864 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2720 TCGA T^CGA
XbaI 895 TCTAGA T^CTAGA
XmaCI 2872 CCCGGG C^CCGGG
XmaI 2872 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AhdI 1451 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2188 GACACAAGGTC
AocI 1158 CCTCAGG CC^T[ACGT]AGG
3811 CCTCAGG
AspEI 1451 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2188 GACACAAGGTC
AspHI 2725 GAGCAC G[AT]GC[AT]^C
3090 GAGCTC
BbeI 982 GGCGCC GGCGC^C
1745 GGCGCC
BglI 114 GCCTCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
985 GCCATCTTGGC
BlpI 2426 GCTGAGC GC^T[ACGT]AGC
2527 GCTCAGC
BpmI 2171 CTGGAG CTGGAG(16/14)
2815 CTGGAG
Bpu1102I 2426 GCTGAGC GC^T[ACGT]AGC
2527 GCTCAGC
BsiHKAI 2725 GAGCAC G[AT]GC[AT]^C
3090 GAGCTC
BsmBI 1700 CGTCTC CGTCTC(1/5)
1279 GAGACG
BspHI 2646 TCATGA T^CATGA
3135 TCATGA
BsrFI 2400 ACCGGC [GA]^CCGG[CT]
2799 ACCGGT
BstAPI 1001 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3304 GCACCTTGTGC
Bsu36I 1158 CCTCAGG CC^T[ACGT]AGG
3811 CCTCAGG
CelII 2426 GCTGAGC GC^T[ACGT]AGC
2527 GCTCAGC
Cfr10I 2400 ACCGGC [GA]^CCGG[CT]
2799 ACCGGT
DraIII 373 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
3305 CACCTTGTG
Eam1105I 1451 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2188 GACACAAGGTC
Eco81I 1158 CCTCAGG CC^T[ACGT]AGG
3811 CCTCAGG
EcoNI 3314 CCTAGACTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3807 CCTTCCTCAGG
HaeII 982 GGCGCC [GA]GCGC^[CT]
1745 GGCGCC
HincII 626 GTTAAC GT[CT]^[GA]AC
657 GTTGAC
HindII 626 GTTAAC GT[CT]^[GA]AC
657 GTTGAC
KasI 982 GGCGCC G^GCGCC
1745 GGCGCC
NarI 982 GGCGCC GG^CGCC
1745 GGCGCC
PflMI 3041 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3652 CCAGGACTTGG
PshAI 1254 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2476 GACCATCGTC
RcaI 2646 TCATGA T^CATGA
3135 TCATGA
SfoI 982 GGCGCC GGC^GCC
1745 GGCGCC
Van91I 3041 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3652 CCAGGACTTGG
XcmI 2856 CCACTGAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3350 CCAAGGGCTTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI