################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## Acc65I AclI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbvCI BcgI BfrI BlnI BlpI Bpu1102I BsaBI BspDI BspHI BstAPI BstBI Bsu36I CelII ClaI CpoI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoT22I FseI FspI HpaI KpnI Ksp632I MamI MfeI MunI NotI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI SexAI SfuI SmaI SnaBI SpeI Sse8387I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccIII 729 TCCGGA T^CCGGA ApaI 2400 GGGCCC GGGCC^C Asp700I 871 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 2850 GGATCC G^GATCC BbrPI 1879 CACGTG CAC^GTG BbsI 1135 GAAGAC GAAGAC(2/6) BciVI 2629 GTATCC GTATCC(6/5) BglII 3265 AGATCT A^GATCT BpuAI 1135 GAAGAC GAAGAC(2/6) BsaAI 1879 CACGTG [CT]AC^GT[GA] BseAI 729 TCCGGA T^CCGGA BsiEI 384 CGACCG CG[GA][CT]^CG BsiWI 1937 CGTACG C^GTACG BspEI 729 TCCGGA T^CCGGA BspLU11I 3285 ACATGT A^CATGT BssSI 1687 CACGAG CACGAG(-5/-1) Bst1107I 2206 GTATAC GTA^TAC BstZ17I 2206 GTATAC GTA^TAC DraIII 1879 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRV 595 GATATC GAT^ATC HindIII 4032 AAGCTT A^AGCTT MroI 729 TCCGGA T^CCGGA NcoI 1858 CCATGG C^CATGG NdeI 3934 CATATG CA^TATG NruI 1725 TCGCGA TCG^CGA PmaCI 1879 CACGTG CAC^GTG PmlI 1879 CACGTG CAC^GTG ScaI 807 AGTACT AGT^ACT SfiI 322 GGCCGCGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 503 CGCCGGTG C[GA]^CCGG[CT]G SmlI 3097 CTCAAG C^T[CT][GA]AG SspI 3770 AATATT AAT^ATT SwaI 3522 ATTTAAAT ATTT^AAAT XmnI 871 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AatII 1281 GACGTC GACGT^C 3180 GACGTC BbeI 580 GGCGCC GGCGC^C 2136 GGCGCC BglI 181 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 323 GCCGCGGAGGC BsaI 3156 GAGACC GGTCTC(1/5) 216 GGTCTC BsaWI 729 TCCGGA [AT]^CCGG[AT] 2740 TCCGGT BsgI 33 CTGCAC GTGCAG(16/14) 417 GTGCAG BsmBI 1397 GAGACG CGTCTC(1/5) 2971 GAGACG BsmI 3473 GAATGC GAATGC(1/-1) 2582 GCATTC BsrDI 2217 CATTGC GCAATG(2/0) 3533 CATTGC DrdI 1246 GACATGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3158 GACCCTAAAGTC EcoNI 333 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2568 CCTAGAGGAGG EcoT14I 317 CCAAGG C^C[AT][AT]GG 1858 CCATGG KasI 580 GGCGCC G^GCGCC 2136 GGCGCC KspI 324 CCGCGG CCGC^GG 2941 CCGCGG MluI 1925 ACGCGT A^CGCGT 2014 ACGCGT NaeI 401 GCCGGC GCC^GGC 1732 GCCGGC NarI 580 GGCGCC GG^CGCC 2136 GGCGCC NgoMIV 401 GCCGGC G^CCGGC 1732 GCCGGC NheI 1809 GCTAGC G^CTAGC 3835 GCTAGC PpuMI 263 AGGACCT [GA]G^G[AT]CC[CT] 2175 GGGTCCC SacII 324 CCGCGG CCGC^GG 2941 CCGCGG SfoI 580 GGCGCC GGC^GCC 2136 GGCGCC SphI 1644 GCATGC GCATG^C 2549 GCATGC StuI 330 AGGCCT AGG^CCT 2144 AGGCCT StyI 317 CCAAGG C^C[AT][AT]GG 1858 CCATGG TaqI 383 TCGA T^CGA 1627 TCGA TthHB8I 383 TCGA T^CGA 1627 TCGA XcmI 1430 CCAGTGTTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2814 CCAGTGAGAGGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI ApaLI ApoI AspHI AvaI AvaII BalI BanI BanII BbvI BclI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EcoO109I EcoO65I EcoRI EcoRII FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII