##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BbvCI BcgI BfrI BlnI BlpI
Bpu1102I BsaBI BspDI BspHI BstAPI
BstBI Bsu36I CelII ClaI CpoI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoT22I FseI FspI
HpaI KpnI Ksp632I MamI MfeI
MunI NotI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SbfI SexAI
SfuI SmaI SnaBI SpeI Sse8387I
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 729 TCCGGA T^CCGGA
ApaI 2400 GGGCCC GGGCC^C
Asp700I 871 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 2850 GGATCC G^GATCC
BbrPI 1879 CACGTG CAC^GTG
BbsI 1135 GAAGAC GAAGAC(2/6)
BciVI 2629 GTATCC GTATCC(6/5)
BglII 3265 AGATCT A^GATCT
BpuAI 1135 GAAGAC GAAGAC(2/6)
BsaAI 1879 CACGTG [CT]AC^GT[GA]
BseAI 729 TCCGGA T^CCGGA
BsiEI 384 CGACCG CG[GA][CT]^CG
BsiWI 1937 CGTACG C^GTACG
BspEI 729 TCCGGA T^CCGGA
BspLU11I 3285 ACATGT A^CATGT
BssSI 1687 CACGAG CACGAG(-5/-1)
Bst1107I 2206 GTATAC GTA^TAC
BstZ17I 2206 GTATAC GTA^TAC
DraIII 1879 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 595 GATATC GAT^ATC
HindIII 4032 AAGCTT A^AGCTT
MroI 729 TCCGGA T^CCGGA
NcoI 1858 CCATGG C^CATGG
NdeI 3934 CATATG CA^TATG
NruI 1725 TCGCGA TCG^CGA
PmaCI 1879 CACGTG CAC^GTG
PmlI 1879 CACGTG CAC^GTG
ScaI 807 AGTACT AGT^ACT
SfiI 322 GGCCGCGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 503 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 3097 CTCAAG C^T[CT][GA]AG
SspI 3770 AATATT AAT^ATT
SwaI 3522 ATTTAAAT ATTT^AAAT
XmnI 871 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 1281 GACGTC GACGT^C
3180 GACGTC
BbeI 580 GGCGCC GGCGC^C
2136 GGCGCC
BglI 181 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
323 GCCGCGGAGGC
BsaI 3156 GAGACC GGTCTC(1/5)
216 GGTCTC
BsaWI 729 TCCGGA [AT]^CCGG[AT]
2740 TCCGGT
BsgI 33 CTGCAC GTGCAG(16/14)
417 GTGCAG
BsmBI 1397 GAGACG CGTCTC(1/5)
2971 GAGACG
BsmI 3473 GAATGC GAATGC(1/-1)
2582 GCATTC
BsrDI 2217 CATTGC GCAATG(2/0)
3533 CATTGC
DrdI 1246 GACATGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3158 GACCCTAAAGTC
EcoNI 333 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2568 CCTAGAGGAGG
EcoT14I 317 CCAAGG C^C[AT][AT]GG
1858 CCATGG
KasI 580 GGCGCC G^GCGCC
2136 GGCGCC
KspI 324 CCGCGG CCGC^GG
2941 CCGCGG
MluI 1925 ACGCGT A^CGCGT
2014 ACGCGT
NaeI 401 GCCGGC GCC^GGC
1732 GCCGGC
NarI 580 GGCGCC GG^CGCC
2136 GGCGCC
NgoMIV 401 GCCGGC G^CCGGC
1732 GCCGGC
NheI 1809 GCTAGC G^CTAGC
3835 GCTAGC
PpuMI 263 AGGACCT [GA]G^G[AT]CC[CT]
2175 GGGTCCC
SacII 324 CCGCGG CCGC^GG
2941 CCGCGG
SfoI 580 GGCGCC GGC^GCC
2136 GGCGCC
SphI 1644 GCATGC GCATG^C
2549 GCATGC
StuI 330 AGGCCT AGG^CCT
2144 AGGCCT
StyI 317 CCAAGG C^C[AT][AT]GG
1858 CCATGG
TaqI 383 TCGA T^CGA
1627 TCGA
TthHB8I 383 TCGA T^CGA
1627 TCGA
XcmI 1430 CCAGTGTTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2814 CCAGTGAGAGGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI ApaLI ApoI AspHI AvaI
AvaII BalI BanI BanII BbvI
BclI BcnI BfaI BmyI BpmI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrFI BsrGI
BsrI BssHII BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EcoO109I EcoO65I EcoRI EcoRII FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XhoII