##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

Acc65I      AclI        AflII       AgeI        AhdI        
AocI        Aor51HI     AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BbvCI       BcgI        BfrI        BlnI        BlpI        
Bpu1102I    BsaBI       BspDI       BspHI       BstAPI      
BstBI       Bsu36I      CelII       ClaI        CpoI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoT22I     FseI        FspI        
HpaI        KpnI        Ksp632I     MamI        MfeI        
MunI        NotI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SalI        SapI        SbfI        SexAI       
SfuI        SmaI        SnaBI       SpeI        Sse8387I    
Tth111I     XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AccIII        729  TCCGGA               T^CCGGA
ApaI         2400  GGGCCC               GGGCC^C
Asp700I       871  GAAAATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI        2850  GGATCC               G^GATCC
BbrPI        1879  CACGTG               CAC^GTG
BbsI         1135  GAAGAC               GAAGAC(2/6)
BciVI        2629  GTATCC               GTATCC(6/5)
BglII        3265  AGATCT               A^GATCT
BpuAI        1135  GAAGAC               GAAGAC(2/6)
BsaAI        1879  CACGTG               [CT]AC^GT[GA]
BseAI         729  TCCGGA               T^CCGGA
BsiEI         384  CGACCG               CG[GA][CT]^CG
BsiWI        1937  CGTACG               C^GTACG
BspEI         729  TCCGGA               T^CCGGA
BspLU11I     3285  ACATGT               A^CATGT
BssSI        1687  CACGAG               CACGAG(-5/-1)
Bst1107I     2206  GTATAC               GTA^TAC
BstZ17I      2206  GTATAC               GTA^TAC
DraIII       1879  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRV         595  GATATC               GAT^ATC
HindIII      4032  AAGCTT               A^AGCTT
MroI          729  TCCGGA               T^CCGGA
NcoI         1858  CCATGG               C^CATGG
NdeI         3934  CATATG               CA^TATG
NruI         1725  TCGCGA               TCG^CGA
PmaCI        1879  CACGTG               CAC^GTG
PmlI         1879  CACGTG               CAC^GTG
ScaI          807  AGTACT               AGT^ACT
SfiI          322  GGCCGCGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI         503  CGCCGGTG             C[GA]^CCGG[CT]G
SmlI         3097  CTCAAG               C^T[CT][GA]AG
SspI         3770  AATATT               AAT^ATT
SwaI         3522  ATTTAAAT             ATTT^AAAT
XmnI          871  GAAAATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AatII        1281  GACGTC               GACGT^C
             3180  GACGTC              
BbeI          580  GGCGCC               GGCGC^C
             2136  GGCGCC              
BglI          181  GCCTGGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              323  GCCGCGGAGGC         
BsaI         3156  GAGACC               GGTCTC(1/5)
              216  GGTCTC              
BsaWI         729  TCCGGA               [AT]^CCGG[AT]
             2740  TCCGGT              
BsgI           33  CTGCAC               GTGCAG(16/14)
              417  GTGCAG              
BsmBI        1397  GAGACG               CGTCTC(1/5)
             2971  GAGACG              
BsmI         3473  GAATGC               GAATGC(1/-1)
             2582  GCATTC              
BsrDI        2217  CATTGC               GCAATG(2/0)
             3533  CATTGC              
DrdI         1246  GACATGGGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3158  GACCCTAAAGTC        
EcoNI         333  CCTTCTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2568  CCTAGAGGAGG         
EcoT14I       317  CCAAGG               C^C[AT][AT]GG
             1858  CCATGG              
KasI          580  GGCGCC               G^GCGCC
             2136  GGCGCC              
KspI          324  CCGCGG               CCGC^GG
             2941  CCGCGG              
MluI         1925  ACGCGT               A^CGCGT
             2014  ACGCGT              
NaeI          401  GCCGGC               GCC^GGC
             1732  GCCGGC              
NarI          580  GGCGCC               GG^CGCC
             2136  GGCGCC              
NgoMIV        401  GCCGGC               G^CCGGC
             1732  GCCGGC              
NheI         1809  GCTAGC               G^CTAGC
             3835  GCTAGC              
PpuMI         263  AGGACCT              [GA]G^G[AT]CC[CT]
             2175  GGGTCCC             
SacII         324  CCGCGG               CCGC^GG
             2941  CCGCGG              
SfoI          580  GGCGCC               GGC^GCC
             2136  GGCGCC              
SphI         1644  GCATGC               GCATG^C
             2549  GCATGC              
StuI          330  AGGCCT               AGG^CCT
             2144  AGGCCT              
StyI          317  CCAAGG               C^C[AT][AT]GG
             1858  CCATGG              
TaqI          383  TCGA                 T^CGA
             1627  TCGA                
TthHB8I       383  TCGA                 T^CGA
             1627  TCGA                
XcmI         1430  CCAGTGTTTCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2814  CCAGTGAGAGGTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AluI        Alw44I      AlwI        
AlwNI       ApaLI       ApoI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbvI        
BclI        BcnI        BfaI        BmyI        BpmI        
BsaHI       BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
Bsp1407I    BspMI       BsrBI       BsrFI       BsrGI       
BsrI        BssHII      BssKI       BstEII      BstF5I      
BstNI       BstPI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EcoO109I    EcoO65I     EcoRI       EcoRII      FauI        
FbaI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SspBI       TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Van91I      
XhoII