##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI AscI AseI AsnI Asp718I
AviII AvrII BbrPI BlnI BseAI
BsiEI BsiWI Bsp1407I BspEI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Cfr10I CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoT22I FseI FspI HpaI KpnI
KspI MluI MroI NaeI NcoI
NgoMIV NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SapI SbfI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AflII 4323 CTTAAG C^TTAAG
AflIII 2988 ACATGT A^C[GA][CT]GT
AhdI 4019 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4186 GTGCAC G^TGCAC
AlwNI 4113 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1961 CCTAAGG CC^T[ACGT]AGG
ApaI 58 GGGCCC GGGCC^C
ApaLI 4186 GTGCAC G^TGCAC
AspEI 4019 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4198 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2129 TGGCCA TGG^CCA
BamHI 4210 GGATCC G^GATCC
BbeI 209 GGCGCC GGCGC^C
BbvCI 1531 CCTCAGC CCTCAGC(-5/-2)
BciVI 3193 GGATAC GTATCC(6/5)
BclI 3526 TGATCA T^GATCA
BfrI 4323 CTTAAG C^TTAAG
BsaAI 4136 CACGTA [CT]AC^GT[GA]
BsaBI 1647 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3535 ACCGGA [AT]^CCGG[AT]
BspDI 1841 ATCGAT AT^CGAT
BspHI 626 TCATGA T^CATGA
BspLU11I 2988 ACATGT A^CATGT
BstEII 193 GGTGACC G^GT[ACGT]ACC
BstPI 193 GGTGACC G^GT[ACGT]ACC
BstXI 1940 CCACAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1961 CCTAAGG CC^T[ACGT]AGG
ClaI 1841 ATCGAT AT^CGAT
DraI 3070 TTTAAA TTT^AAA
DraIII 670 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2129 TGGCCA [CT]^GGCC[GA]
Eam1105I 4019 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1961 CCTAAGG CC^T[ACGT]AGG
EcoO65I 193 GGTGACC G^GT[ACGT]ACC
EcoRV 2347 GATATC GAT^ATC
EcoT14I 3809 CCAAGG C^C[AT][AT]GG
FbaI 3526 TGATCA T^GATCA
HincII 1774 GTCGAC GT[CT]^[GA]AC
HindII 1774 GTCGAC GT[CT]^[GA]AC
HindIII 69 AAGCTT A^AGCTT
KasI 209 GGCGCC G^GCGCC
MamI 1647 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1004 CAATTG C^AATTG
MluNI 2129 TGGCCA TGG^CCA
MscI 2129 TGGCCA TGG^CCA
MunI 1004 CAATTG C^AATTG
NarI 209 GGCGCC GG^CGCC
NdeI 2167 CATATG CA^TATG
NheI 2581 GCTAGC G^CTAGC
PflFI 4198 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 626 TCATGA T^CATGA
SalI 1774 GTCGAC G^TCGAC
SexAI 1317 ACCAGGT A^CC[AT]GGT
SfiI 2893 GGCCAGAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 209 GGCGCC GGC^GCC
StuI 2901 AGGCCT AGG^CCT
StyI 3809 CCAAGG C^C[AT][AT]GG
Tth111I 4198 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2187 CCATCTGAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 209 GGCGCC G[GA]^CG[CT]C
1331 GGCGTC
Asp700I 1246 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3349 GAAAACCTTC
BcgI 368 CGAGCGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2282 GCAGCTGTATCG
BglI 1536 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2894 GCCAGAGAGGC
BglII 1514 AGATCT A^GATCT
2228 AGATCT
BlpI 1923 GCTCAGC GC^T[ACGT]AGC
3580 GCTGAGC
Bpu1102I 1923 GCTCAGC GC^T[ACGT]AGC
3580 GCTGAGC
BsaHI 209 GGCGCC G[GA]^CG[CT]C
1331 GGCGTC
BsaI 1427 GGTCTC GGTCTC(1/5)
2945 GGTCTC
BsgI 2924 CTGCAC GTGCAG(16/14)
4085 GTGCAG
BsmI 4171 GAATGC GAATGC(1/-1)
3838 GCATTC
BspMI 2322 ACCTGC ACCTGC(4/8)
3426 ACCTGC
CelII 1923 GCTCAGC GC^T[ACGT]AGC
3580 GCTGAGC
DraII 3237 GGGTCCC [GA]G^G[ACGT]CC[CT]
4202 AGGTCCC
DsaI 222 CCGTGG C^C[GA][CT]GG
957 CCGTGG
EarI 1951 GAAGAG CTCTTC(1/4)
3476 GAAGAG
EcoO109I 3237 GGGTCCC [GA]G^G[ACGT]CC[CT]
4202 AGGTCCC
EcoRI 1677 GAATTC G^AATTC
2433 GAATTC
HaeII 209 GGCGCC [GA]GCGC^[CT]
370 AGCGCC
HgaI 288 GACGC GACGC(5/10)
1332 GCGTC
Hin1I 209 GGCGCC G[GA]^CG[CT]C
1331 GGCGTC
Ksp632I 1951 GAAGAG CTCTTC(1/4)
3476 GAAGAG
PaeR7I 366 CTCGAG C^TCGAG
3277 CTCGAG
PpuMI 3237 GGGTCCC [GA]G^G[AT]CC[CT]
4202 AGGTCCC
SacI 163 GAGCTC GAGCT^C
1462 GAGCTC
ScaI 741 AGTACT AGT^ACT
1662 AGTACT
SpeI 2124 ACTAGT A^CTAGT
3734 ACTAGT
XhoI 366 CTCGAG C^TCGAG
3277 CTCGAG
XmnI 1246 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3349 GAAAACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EcoNI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII