##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccI AccIII AflII AgeI
Aor51HI AscI AseI AsnI AviII
BamHI BciVI BfrI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrFI BsrGI BssHII Bst1107I
BstZ17I Cfr10I ClaI CpoI EagI
EclXI Eco47III Eco52I EcoRV EcoT22I
FseI FspI HincII HindII HpaI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PinAI PmeI
PshBI PvuI RsrII SacI SalI
SapI SbfI SexAI SfiI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 790 GGTACC G^GTACC
AccII 2075 CGCG CG^CG
AclI 2388 AACGTT AA^CGTT
AhdI 1479 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3747 GGGCCC GGGCC^C
Asp718I 790 GGTACC G^GTACC
AspEI 1479 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1264 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 303 CCTAGG C^CTAGG
BbeI 405 GGCGCC GGCGC^C
BbrPI 1051 CACGTG CAC^GTG
BclI 1579 TGATCA T^GATCA
BglI 460 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2551 AGATCT A^GATCT
BlnI 303 CCTAGG C^CTAGG
BlpI 3503 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3503 GCTGAGC GC^T[ACGT]AGC
BsaBI 2711 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BssSI 1802 CACGAG CACGAG(-5/-1)
BstEII 3961 GGTGACC G^GT[ACGT]ACC
BstPI 3961 GGTGACC G^GT[ACGT]ACC
BstUI 2075 CGCG CG^CG
CelII 3503 GCTGAGC GC^T[ACGT]AGC
DraI 670 TTTAAA TTT^AAA
Eam1105I 1479 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2201 CCTGTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3961 GGTGACC G^GT[ACGT]ACC
FbaI 1579 TGATCA T^GATCA
KasI 405 GGCGCC G^GCGCC
KpnI 790 GGTACC GGTAC^C
KspI 2074 CCGCGG CCGC^GG
MamI 2711 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2075 CGCG CG^CG
NarI 405 GGCGCC GG^CGCC
PflFI 1264 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3146 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1051 CACGTG CAC^GTG
PmlI 1051 CACGTG CAC^GTG
PshAI 3668 GACCGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2388 AACGTT AA^CGTT
SacII 2074 CCGCGG CCGC^GG
SfoI 405 GGCGCC GGC^GCC
StuI 1783 AGGCCT AGG^CCT
Tth111I 1264 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3146 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1801 CCACGAGACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 1052 ACGTGT A^C[GA][CT]GT
1338 ACGTGT
Alw44I 1410 GTGCAC G^TGCAC
2009 GTGCAC
ApaLI 1410 GTGCAC G^TGCAC
2009 GTGCAC
BbvCI 488 CCTCAGC CCTCAGC(-5/-2)
3134 CCTCAGC
BcgI 1050 GCACGTGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3346 GCAACACTCTCG
BsaAI 1051 CACGTG [CT]AC^GT[GA]
1182 TACGTG
BsaI 997 GAGACC GGTCTC(1/5)
1805 GAGACC
BsmBI 1269 CGTCTC CGTCTC(1/5)
2422 GAGACG
BsmI 1217 GAATGC GAATGC(1/-1)
1397 GCATTC
BspHI 1582 TCATGA T^CATGA
3541 TCATGA
BspMI 2356 ACCTGC ACCTGC(4/8)
3337 ACCTGC
BsrBI 1704 CCGCTC CCGCTC(-3/-3)
1731 GAGCGG
BstAPI 769 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1829 GCAGCACTTGC
BstBI 1058 TTCGAA TT^CGAA
2457 TTCGAA
DraIII 1413 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
1973 CACCATGTG
DrdI 2424 GACGTGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3531 GACCTGGGGGTC
EcoRI 483 GAATTC G^AATTC
3260 GAATTC
HindIII 2409 AAGCTT A^AGCTT
3408 AAGCTT
NspI 1853 GCATGT [GA]CATG^[CT]
2812 ACATGC
NspV 1058 TTCGAA TT^CGAA
2457 TTCGAA
RcaI 1582 TCATGA T^CATGA
3541 TCATGA
ScaI 1894 AGTACT AGT^ACT
1906 AGTACT
SfuI 1058 TTCGAA TT^CGAA
2457 TTCGAA
SmaI 353 CCCGGG CCC^GGG
2113 CCCGGG
XmaCI 353 CCCGGG C^CCGGG
2113 CCCGGG
XmaI 353 CCCGGG C^CCGGG
2113 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI