##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AcsI AflII AgeI ApoI
AscI Asp718I BamHI BbeI BciVI
BfrI BglII BsaBI BsiWI Bsp1407I
BspDI BspLU11I BsrGI Bst1107I BstBI
BstZ17I ClaI CpoI EcoRI EcoRV
EcoT22I FseI HindIII HpaI KasI
KpnI MamI MfeI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshAI
RsrII SalI SapI SfoI SfuI
SnaBI SpeI SphI SspBI SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 3024 GACGTC GACGT^C
AccIII 3637 TCCGGA T^CCGGA
AclI 795 AACGTT AA^CGTT
AhdI 1209 GACATGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4839 AGCGCT AGC^GCT
Asp700I 3455 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1209 GACATGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2127 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1515 CCTAGG C^CTAGG
BbrPI 801 CACGTG CAC^GTG
BclI 2350 TGATCA T^GATCA
BfaI 1516 CTAG C^TAG
BlnI 1515 CCTAGG C^CTAGG
BsaAI 801 CACGTG [CT]AC^GT[GA]
BsaWI 3637 TCCGGA [AT]^CCGG[AT]
BseAI 3637 TCCGGA T^CCGGA
BsmI 4866 GAATGC GAATGC(1/-1)
BspEI 3637 TCCGGA T^CCGGA
BssHII 402 GCGCGC G^CGCGC
BssSI 2514 CACGAG CACGAG(-5/-1)
DraI 4718 TTTAAA TTT^AAA
DrdI 12 GACGTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1209 GACATGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4839 AGCGCT AGC^GCT
FbaI 2350 TGATCA T^GATCA
MaeI 1516 CTAG C^TAG
MroI 3637 TCCGGA T^CCGGA
PaeR7I 3346 CTCGAG C^TCGAG
PflFI 2127 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 801 CACGTG CAC^GTG
PmlI 801 CACGTG CAC^GTG
Psp1406I 795 AACGTT AA^CGTT
PvuI 370 CGATCG CGAT^CG
SacI 258 GAGCTC GAGCT^C
SexAI 1297 ACCAGGT A^CC[AT]GGT
SfiI 4493 GGCCTCACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 147 CACCGGCG C[GA]^CCGG[CT]G
SmaI 1894 CCCGGG CCC^GGG
TfiI 4194 GATTC G^A[AT]TC
Tth111I 2127 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3346 CTCGAG C^TCGAG
XmaCI 1894 CCCGGG C^CCGGG
XmaI 1894 CCCGGG C^CCGGG
XmnI 3455 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1441 GTCTAC GT^[AC][GT]AC
3186 GTCTAC
AocI 825 CCTGAGG CC^T[ACGT]AGG
1446 CCTGAGG
AseI 2223 ATTAAT AT^TAAT
3090 ATTAAT
AsnI 2223 ATTAAT AT^TAAT
3090 ATTAAT
BcgI 1138 GCAAAGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3202 GCACCTCCATCG
BlpI 3940 GCTTAGC GC^T[ACGT]AGC
4512 GCTCAGC
Bpu1102I 3940 GCTTAGC GC^T[ACGT]AGC
4512 GCTCAGC
BsmBI 242 CGTCTC CGTCTC(1/5)
511 GAGACG
BspHI 2347 TCATGA T^CATGA
2804 TCATGA
BsrDI 1589 CATTGC GCAATG(2/0)
1063 GCAATG
BstEII 2244 GGTTACC G^GT[ACGT]ACC
2948 GGTCACC
BstPI 2244 GGTTACC G^GT[ACGT]ACC
2948 GGTCACC
BstYI 604 AGATCC [GA]^GATC[CT]
4392 GGATCT
Bsu36I 825 CCTGAGG CC^T[ACGT]AGG
1446 CCTGAGG
CelII 3940 GCTTAGC GC^T[ACGT]AGC
4512 GCTCAGC
Eco81I 825 CCTGAGG CC^T[ACGT]AGG
1446 CCTGAGG
EcoO65I 2244 GGTTACC G^GT[ACGT]ACC
2948 GGTCACC
KspI 58 CCGCGG CCGC^GG
1274 CCGCGG
MflI 604 AGATCC [GA]^GATC[CT]
4392 GGATCT
NspI 1210 ACATGC [GA]CATG^[CT]
4156 GCATGT
PshBI 2223 ATTAAT AT^TAAT
3090 ATTAAT
RcaI 2347 TCATGA T^CATGA
2804 TCATGA
SacII 58 CCGCGG CCGC^GG
1274 CCGCGG
SbfI 1838 CCTGCAGG CCTGCA^GG
3979 CCTGCAGG
ScaI 3262 AGTACT AGT^ACT
3706 AGTACT
Sse8387I 1838 CCTGCAGG CCTGCA^GG
3979 CCTGCAGG
XhoII 604 AGATCC [GA]^GATC[CT]
4392 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII AviII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MluI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI