##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
Alw44I ApaI ApaLI AscI AviII
AvrII BamHI BbeI BbvCI BcgI
BlnI BsaHI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BsrBI BsrFI
BssHII Bst1107I BstBI BstZ17I Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco52I FseI FspI HgaI
Hin1I HpaI KasI KspI MroI
NaeI NarI NcoI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I StuI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 1359 GGTACC G^GTACC
AccI 2756 GTCTAC GT^[AC][GT]AC
AflII 2253 CTTAAG C^TTAAG
AhdI 1926 GACATGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3738 CCTTAGG CC^T[ACGT]AGG
Aor51HI 764 AGCGCT AGC^GCT
Asp718I 1359 GGTACC G^GTACC
AspEI 1926 GACATGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 105 GAGCTC G[AT]GC[AT]^C
AspI 1731 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1371 TGGCCA TGG^CCA
BbrPI 2833 CACGTG CAC^GTG
BciVI 4587 GTATCC GTATCC(6/5)
BclI 494 TGATCA T^GATCA
BfrI 2253 CTTAAG C^TTAAG
BglI 2283 GCCGTATCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 293 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 293 GCTGAGC GC^T[ACGT]AGC
BsaAI 2833 CACGTG [CT]AC^GT[GA]
BsaBI 4582 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 105 GAGCTC G[AT]GC[AT]^C
BsmBI 209 GAGACG CGTCTC(1/5)
Bsp1407I 601 TGTACA T^GTACA
BspLU11I 1021 ACATGT A^CATGT
BsrGI 601 TGTACA T^GTACA
BstAPI 220 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2344 CCAACCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3738 CCTTAGG CC^T[ACGT]AGG
CelII 293 GCTGAGC GC^T[ACGT]AGC
CfoI 765 GCGC GCG^C
Eam1105I 1926 GACATGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 764 AGCGCT AGC^GCT
Eco81I 3738 CCTTAGG CC^T[ACGT]AGG
EcoNI 1350 CCTCTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1959 GATATC GAT^ATC
EcoT14I 3471 CCAAGG C^C[AT][AT]GG
EcoT22I 2711 ATGCAT ATGCA^T
FbaI 494 TGATCA T^GATCA
HaeII 764 AGCGCT [GA]GCGC^[CT]
HhaI 765 GCGC GCG^C
HinP1I 765 GCGC G^CGC
KpnI 1359 GGTACC GGTAC^C
MamI 4582 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1645 CAATTG C^AATTG
MluI 2081 ACGCGT A^CGCGT
MluNI 1371 TGGCCA TGG^CCA
MscI 1371 TGGCCA TGG^CCA
MunI 1645 CAATTG C^AATTG
NsiI 2711 ATGCAT ATGCA^T
PflFI 1731 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2833 CACGTG CAC^GTG
PmlI 2833 CACGTG CAC^GTG
PstI 760 CTGCAG CTGCA^G
PvuII 2803 CAGCTG CAG^CTG
SacI 105 GAGCTC GAGCT^C
SmaI 51 CCCGGG CCC^GGG
SspBI 601 TGTACA T^GTACA
StyI 3471 CCAAGG C^C[AT][AT]GG
Tth111I 1731 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 51 CCCGGG C^CCGGG
XmaI 51 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 1140 CGCG CG^CG
2082 CGCG
AflIII 1021 ACATGT A^C[GA][CT]GT
2081 ACGCGT
AlwNI 221 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
2647 CAGAGACTG
AseI 2094 ATTAAT AT^TAAT
4434 ATTAAT
AsnI 2094 ATTAAT AT^TAAT
4434 ATTAAT
Asp700I 1896 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4534 GAATTGCTTC
AvaI 51 CCCGGG C^[CT]CG[GA]G
100 CCCGAG
BanI 800 GGTGCC G^G[CT][GA]CC
1359 GGTACC
BglII 653 AGATCT A^GATCT
3264 AGATCT
BsoBI 51 CCCGGG C^[CT]CG[GA]G
100 CCCGAG
BspHI 550 TCATGA T^CATGA
1904 TCATGA
BspMI 225 ACCTGC ACCTGC(4/8)
3476 GCAGGT
BstEII 2407 GGTGACC G^GT[ACGT]ACC
3541 GGTCACC
BstPI 2407 GGTGACC G^GT[ACGT]ACC
3541 GGTCACC
BstUI 1140 CGCG CG^CG
2082 CGCG
BstYI 653 AGATCT [GA]^GATC[CT]
3264 AGATCT
DsaI 2185 CCGTGG C^C[GA][CT]GG
2674 CCGTGG
EarI 9 GAAGAG CTCTTC(1/4)
2141 GAAGAG
EcoO65I 2407 GGTGACC G^GT[ACGT]ACC
3541 GGTCACC
EcoRI 997 GAATTC G^AATTC
4313 GAATTC
HincII 436 GTCAAC GT[CT]^[GA]AC
2536 GTCAAC
HindII 436 GTCAAC GT[CT]^[GA]AC
2536 GTCAAC
Ksp632I 9 GAAGAG CTCTTC(1/4)
2141 GAAGAG
MaeII 1165 ACGT A^CGT
2834 ACGT
MflI 653 AGATCT [GA]^GATC[CT]
3264 AGATCT
MspA1I 133 CCGCTG C[AC]G^C[GT]G
2803 CAGCTG
MvnI 1140 CGCG CG^CG
2082 CGCG
NheI 909 GCTAGC G^CTAGC
3169 GCTAGC
NspI 1021 ACATGT [GA]CATG^[CT]
3821 ACATGC
PshBI 2094 ATTAAT AT^TAAT
4434 ATTAAT
RcaI 550 TCATGA T^CATGA
1904 TCATGA
SmlI 1067 CTTGAG C^T[CT][GA]AG
2253 CTTAAG
SspI 2978 AATATT AAT^ATT
4321 AATATT
XcmI 980 CCAGCCTGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3797 CCACAACAACCCTGG
XhoII 653 AGATCT [GA]^GATC[CT]
3264 AGATCT
XmnI 1896 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4534 GAATTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrDI BsrI BssKI BssSI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraI DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HindIII HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NciI NdeI NdeII NlaIII NlaIV
PflMI PleI PpuMI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI