##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII AccII AclI AgeI AhdI
AocI Aor51HI ApaI AscI AspEI
AspI AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsiEI BsiWI BsmBI BspDI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I FauI FseI FspI HaeII
HhaI HinP1I HpaI KasI KspI
MluI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspV
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 3396 GGTACC G^GTACC
AccI 1715 GTCTAC GT^[AC][GT]AC
AccIII 671 TCCGGA T^CCGGA
AcyI 3785 GGCGTC G[GA]^CG[CT]C
AflII 3238 CTTAAG C^TTAAG
Alw44I 543 GTGCAC G^TGCAC
AlwI 274 GATCC GGATC(4/5)
AlwNI 1490 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 543 GTGCAC G^TGCAC
Asp700I 3169 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3396 GGTACC G^GTACC
AspHI 543 GTGCAC G[AT]GC[AT]^C
BalI 1530 TGGCCA TGG^CCA
BcnI 2693 CCCGG CC^[GC]GG
BfrI 3238 CTTAAG C^TTAAG
BsaAI 2006 TACGTG [CT]AC^GT[GA]
BsaHI 3785 GGCGTC G[GA]^CG[CT]C
BseAI 671 TCCGGA T^CCGGA
BseRI 2060 CTCCTC GAGGAG(10/8)
BsgI 793 GTGCAG GTGCAG(16/14)
BsiHKAI 543 GTGCAC G[AT]GC[AT]^C
BsmFI 2691 GTCCC GGGAC(10/14)
Bsp1407I 570 TGTACA T^GTACA
BspEI 671 TCCGGA T^CCGGA
BspHI 3865 TCATGA T^CATGA
BspLU11I 3949 ACATGT A^CATGT
BsrGI 570 TGTACA T^GTACA
BstAPI 2719 GCACAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 273 AGATCC [GA]^GATC[CT]
DraII 1908 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 238 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1730 GACCACTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1530 TGGCCA [CT]^GGCC[GA]
EarI 3358 GAAGAG CTCTTC(1/4)
EcoO109I 1908 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 2447 GATATC GAT^ATC
EcoT22I 2922 ATGCAT ATGCA^T
HgaI 3786 GCGTC GACGC(5/10)
Hin1I 3785 GGCGTC G[GA]^CG[CT]C
KpnI 3396 GGTACC GGTAC^C
Ksp632I 3358 GAAGAG CTCTTC(1/4)
MaeII 2007 ACGT A^CGT
MfeI 1246 CAATTG C^AATTG
MflI 273 AGATCC [GA]^GATC[CT]
MluNI 1530 TGGCCA TGG^CCA
MroI 671 TCCGGA T^CCGGA
MscI 1530 TGGCCA TGG^CCA
MunI 1246 CAATTG C^AATTG
NciI 2693 CCCGG CC^[GC]GG
NsiI 2922 ATGCAT ATGCA^T
PacI 82 TTAATTAA TTAAT^TAA
PaeR7I 1854 CTCGAG C^TCGAG
PpuMI 1908 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 3865 TCATGA T^CATGA
SspBI 570 TGTACA T^GTACA
TaqI 1855 TCGA T^CGA
TthHB8I 1855 TCGA T^CGA
XbaI 270 TCTAGA T^CTAGA
XhoI 1854 CTCGAG C^TCGAG
XhoII 273 AGATCC [GA]^GATC[CT]
XmnI 3169 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 2007 ACGTGT A^C[GA][CT]GT
3949 ACATGT
AseI 1723 ATTAAT AT^TAAT
3426 ATTAAT
AsnI 1723 ATTAAT AT^TAAT
3426 ATTAAT
AvaI 1854 CTCGAG C^[CT]CG[GA]G
2542 CTCGGG
BanI 3343 GGCACC G^G[CT][GA]CC
3396 GGTACC
BbsI 639 GAAGAC GAAGAC(2/6)
2869 GTCTTC
BpuAI 639 GAAGAC GAAGAC(2/6)
2869 GTCTTC
BsaI 1864 GAGACC GGTCTC(1/5)
2539 GGTCTC
BsaWI 671 TCCGGA [AT]^CCGG[AT]
2536 TCCGGT
BsmI 1483 GAATGC GAATGC(1/-1)
3144 GCATTC
BsoBI 1854 CTCGAG C^[CT]CG[GA]G
2542 CTCGGG
BstNI 1441 CCAGG CC^[AT]GG
1594 CCTGG
EcoRII 1441 CCAGG ^CC[AT]GG
1594 CCTGG
HindIII 351 AAGCTT A^AGCTT
3535 AAGCTT
MspA1I 1838 CAGCTG C[AC]G^C[GT]G
3317 CAGCTG
MvaI 1441 CCAGG CC^[AT]GG
1594 CCTGG
NdeI 434 CATATG CA^TATG
861 CATATG
PflMI 1489 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2754 CCAAGGAATGG
PshBI 1723 ATTAAT AT^TAAT
3426 ATTAAT
PstI 2072 CTGCAG CTGCA^G
3733 CTGCAG
PvuII 1838 CAGCTG CAG^CTG
3317 CAGCTG
SmlI 1854 CTCGAG C^T[CT][GA]AG
3238 CTTAAG
Van91I 1489 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2754 CCAAGGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BclI BfaI BmyI
BsaBI BsaJI BsiYI BslI BsmAI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I Cac8I Cfr13I DdeI DpnII
DraI EcoRI EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeIII MamI MboI MboII MnlI
MseI MslI MspI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI