##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
ApaI AscI Asp718I AspI AviII
BbeI BbrPI BfrI BglII BseAI
BsiWI BspDI BspEI BspMI BsrBI
BsrFI BssHII BstAPI BstBI Cfr10I
ClaI CpoI EcoRV FseI FspI
HpaI KasI KpnI KspI MluI
MroI NaeI NarI NdeI NgoMIV
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI ScaI SfiI SfoI SfuI
SgrAI SmaI SspI SwaI Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccII 916 CGCG CG^CG
Alw44I 4487 GTGCAC G^TGCAC
AocI 1078 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2568 AGCGCT AGC^GCT
ApaLI 4487 GTGCAC G^TGCAC
Asp700I 346 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 3204 CTCGAG C^[CT]CG[GA]G
BalI 2826 TGGCCA TGG^CCA
BbsI 1990 GTCTTC GAAGAC(2/6)
BclI 1654 TGATCA T^GATCA
BlpI 3888 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3888 GCTCAGC GC^T[ACGT]AGC
BpuAI 1990 GTCTTC GAAGAC(2/6)
BsaAI 2391 TACGTA [CT]AC^GT[GA]
BsaBI 3113 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2063 CGGCCG CG[GA][CT]^CG
BsmI 3193 GAATGC GAATGC(1/-1)
BsoBI 3204 CTCGAG C^[CT]CG[GA]G
Bst1107I 670 GTATAC GTA^TAC
BstEII 1375 GGTCACC G^GT[ACGT]ACC
BstPI 1375 GGTCACC G^GT[ACGT]ACC
BstUI 916 CGCG CG^CG
BstXI 4662 CCAGAGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 670 GTATAC GTA^TAC
Bsu36I 1078 CCTGAGG CC^T[ACGT]AGG
CelII 3888 GCTCAGC GC^T[ACGT]AGC
DraI 4912 TTTAAA TTT^AAA
DrdI 2109 GACTAAAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2063 CGGCCG C^GGCCG
EclXI 2063 CGGCCG C^GGCCG
Eco47III 2568 AGCGCT AGC^GCT
Eco52I 2063 CGGCCG C^GGCCG
Eco81I 1078 CCTGAGG CC^T[ACGT]AGG
EcoNI 3284 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1375 GGTCACC G^GT[ACGT]ACC
EcoRI 790 GAATTC G^AATTC
EcoT22I 1778 ATGCAT ATGCA^T
FbaI 1654 TGATCA T^GATCA
HaeII 2568 AGCGCT [GA]GCGC^[CT]
HgaI 2116 GCGTC GACGC(5/10)
HindIII 879 AAGCTT A^AGCTT
MamI 3113 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4706 CAATTG C^AATTG
MluNI 2826 TGGCCA TGG^CCA
MscI 2826 TGGCCA TGG^CCA
MunI 4706 CAATTG C^AATTG
MvnI 916 CGCG CG^CG
NcoI 3559 CCATGG C^CATGG
NheI 4256 GCTAGC G^CTAGC
NsiI 1778 ATGCAT ATGCA^T
PaeR7I 3204 CTCGAG C^TCGAG
SbfI 4267 CCTGCAGG CCTGCA^GG
SnaBI 2391 TACGTA TAC^GTA
SpeI 2023 ACTAGT A^CTAGT
SphI 1780 GCATGC GCATG^C
Sse8387I 4267 CCTGCAGG CCTGCA^GG
StuI 4425 AGGCCT AGG^CCT
XbaI 2215 TCTAGA T^CTAGA
XhoI 3204 CTCGAG C^TCGAG
XmnI 346 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AatII 99 GACGTC GACGT^C
2520 GACGTC
AcyI 99 GACGTC G[GA]^CG[CT]C
2520 GACGTC
AflIII 6 ACATGT A^C[GA][CT]GT
2433 ACATGT
AhdI 4384 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4694 GACTTGTGGTC
AseI 60 ATTAAT AT^TAAT
980 ATTAAT
AsnI 60 ATTAAT AT^TAAT
980 ATTAAT
AspEI 4384 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4694 GACTTGTGGTC
AspHI 1196 GTGCTC G[AT]GC[AT]^C
4487 GTGCAC
BanI 3554 GGTGCC G^G[CT][GA]CC
4658 GGTGCC
BcgI 3547 CGACACTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4627 CGAGCATATTGC
BcnI 1983 CCCGG CC^[GC]GG
2061 CCCGG
BglI 258 GCCTAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2050 GCCTTCTAGGC
BsaHI 99 GACGTC G[GA]^CG[CT]C
2520 GACGTC
BsaI 246 GAGACC GGTCTC(1/5)
4060 GAGACC
BsaWI 1068 ACCGGA [AT]^CCGG[AT]
1446 ACCGGA
BsiHKAI 1196 GTGCTC G[AT]GC[AT]^C
4487 GTGCAC
Bsp1407I 907 TGTACA T^GTACA
1283 TGTACA
BspHI 103 TCATGA T^CATGA
1657 TCATGA
BspLU11I 6 ACATGT A^CATGT
2433 ACATGT
BsrGI 907 TGTACA T^GTACA
1283 TGTACA
CfoI 917 GCGC GCG^C
2569 GCGC
DsaI 3559 CCATGG C^C[GA][CT]GG
4471 CCGTGG
EaeI 2063 CGGCCG [CT]^GGCC[GA]
2826 TGGCCA
Eam1105I 4384 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4694 GACTTGTGGTC
FauI 4127 CCCGC CCCGC(4/6)
1816 GCGGG
HhaI 917 GCGC GCG^C
2569 GCGC
Hin1I 99 GACGTC G[GA]^CG[CT]C
2520 GACGTC
HinP1I 917 GCGC G^CGC
2569 GCGC
HincII 2288 GTCAAC GT[CT]^[GA]AC
4794 GTTGAC
HindII 2288 GTCAAC GT[CT]^[GA]AC
4794 GTTGAC
MslI 220 CACCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3260 CATTTGGATG
NciI 1983 CCCGG CC^[GC]GG
2061 CCCGG
PpuMI 3771 AGGTCCT [GA]G^G[AT]CC[CT]
4273 GGGACCC
PshBI 60 ATTAAT AT^TAAT
980 ATTAAT
RcaI 103 TCATGA T^CATGA
1657 TCATGA
SapI 1198 GCTCTTC GCTCTTC(1/4)
2670 GCTCTTC
SspBI 907 TGTACA T^GTACA
1283 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII AvrII
BamHI BanII BbvCI BbvI BciVI
BfaI BlnI BmyI BpmI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I BsrDI BsrI
BssKI BssSI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DraIII EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII