##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
Alw44I Aor51HI ApaLI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BbeI BbrPI BclI BlnI BsaAI
BsiEI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV FbaI FseI FspI HaeII
HindIII HpaI KasI KpnI KspI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 885 TCCGGA T^CCGGA
AflII 4176 CTTAAG C^TTAAG
AhdI 2236 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3652 GGGCCC GGGCC^C
AscI 951 GGCGCGCC GG^CGCGCC
AspEI 2236 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2427 GGATCC G^GATCC
BbvCI 3938 CCTCAGC CCTCAGC(-5/-2)
BcgI 3480 GCAATGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3549 GTATCC GTATCC(6/5)
BfrI 4176 CTTAAG C^TTAAG
BglI 565 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 3231 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 885 TCCGGA T^CCGGA
BsiWI 1901 CGTACG C^GTACG
BsmI 2601 GAATGC GAATGC(1/-1)
BspEI 885 TCCGGA T^CCGGA
BsrBI 759 CCGCTC CCGCTC(-3/-3)
BssHII 952 GCGCGC G^CGCGC
BstAPI 1352 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 408 GACACCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2236 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2631 CCTTGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3823 GAATTC G^AATTC
EcoT22I 3306 ATGCAT ATGCA^T
MamI 3231 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 885 TCCGGA T^CCGGA
NsiI 3306 ATGCAT ATGCA^T
PaeR7I 899 CTCGAG C^TCGAG
PshAI 712 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1730 AGTACT AGT^ACT
SspI 4224 AATATT AAT^ATT
XbaI 3233 TCTAGA T^CTAGA
XhoI 899 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AcsI 3823 GAATTC [GA]^AATT[CT]
4156 AAATTC
ApoI 3823 GAATTC [GA]^AATT[CT]
4156 AAATTC
BsaWI 885 TCCGGA [AT]^CCGG[AT]
1267 TCCGGT
DraIII 371 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
928 CACACGGTG
HincII 2224 GTCAAC GT[CT]^[GA]AC
2575 GTCAAC
HindII 2224 GTCAAC GT[CT]^[GA]AC
2575 GTCAAC
MaeII 2287 ACGT A^CGT
2751 ACGT
MseI 4132 TTAA T^TAA
4177 TTAA
NcoI 1089 CCATGG C^CATGG
2649 CCATGG
PvuII 2309 CAGCTG CAG^CTG
3580 CAGCTG
SexAI 1175 ACCAGGT A^CC[AT]GGT
2896 ACCAGGT
SfaNI 3746 GATGC GCATC(5/9)
3486 GCATC
StuI 329 AGGCCT AGG^CCT
572 AGGCCT
Tru9I 4132 TTAA T^TAA
4177 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfcI
SmaI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI