################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII Acc65I AccII AcyI AgeI Aor51HI ApaI AscI Asp718I AvrII BamHI BbeI BbvCI BcgI BclI BglI BglII BlnI BsaHI BsaI BsiEI BsiWI BspDI BsrBI BsrFI BssHII BssSI BstAPI BstUI BstYI Cfr10I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I EcoT22I FauI FbaI FseI HaeII HgaI Hin1I HpaI KasI KpnI KspI MflI MluI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmeI PvuI RsrII SacII SalI SbfI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I StuI SwaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 71 enzymes) # ################################### AccIII 2672 TCCGGA T^CCGGA AciI 879 GCGG CCGC(-3/-1) AclI 885 AACGTT AA^CGTT AflII 30 CTTAAG C^TTAAG AhdI 1720 GACGACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 4060 GTGCAC G^TGCAC AocI 765 CCTTAGG CC^T[ACGT]AGG ApaLI 4060 GTGCAC G^TGCAC AseI 3667 ATTAAT AT^TAAT AsnI 3667 ATTAAT AT^TAAT AspEI 1720 GACGACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1906 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1664 CCCGGG C^[CT]CG[GA]G AviII 2724 TGCGCA TGC^GCA BalI 2162 TGGCCA TGG^CCA BanII 502 GAGCTC G[GA]GC[CT]^C BbrPI 2896 CACGTG CAC^GTG BciVI 1485 GGATAC GTATCC(6/5) BfrI 30 CTTAAG C^TTAAG BlpI 1438 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1438 GCTAAGC GC^T[ACGT]AGC BsaAI 2896 CACGTG [CT]AC^GT[GA] BsaWI 2672 TCCGGA [AT]^CCGG[AT] BseAI 2672 TCCGGA T^CCGGA BsgI 553 CTGCAC GTGCAG(16/14) BsmBI 2248 GAGACG CGTCTC(1/5) BsmI 3015 GAATGC GAATGC(1/-1) BsoBI 1664 CCCGGG C^[CT]CG[GA]G Bsp1407I 1760 TGTACA T^GTACA BspEI 2672 TCCGGA T^CCGGA BspHI 2636 TCATGA T^CATGA BspLU11I 3783 ACATGT A^CATGT BsrDI 2767 GCAATG GCAATG(2/0) BsrGI 1760 TGTACA T^GTACA Bst1107I 1102 GTATAC GTA^TAC BstXI 2747 CCACGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1102 GTATAC GTA^TAC Bsu36I 765 CCTTAGG CC^T[ACGT]AGG CelII 1438 GCTAAGC GC^T[ACGT]AGC DraII 756 AGGACCC [GA]G^G[ACGT]CC[CT] EaeI 2162 TGGCCA [CT]^GGCC[GA] Eam1105I 1720 GACGACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 765 CCTTAGG CC^T[ACGT]AGG EcoNI 486 CCTTGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 756 AGGACCC [GA]G^G[ACGT]CC[CT] FspI 2724 TGCGCA TGC^GCA HincII 2515 GTTGAC GT[CT]^[GA]AC HindII 2515 GTTGAC GT[CT]^[GA]AC MluNI 2162 TGGCCA TGG^CCA MroI 2672 TCCGGA T^CCGGA MscI 2162 TGGCCA TGG^CCA PflFI 1906 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1686 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2896 CACGTG CAC^GTG PmlI 2896 CACGTG CAC^GTG PpuMI 756 AGGACCC [GA]G^G[AT]CC[CT] PshAI 2894 GACACGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3667 ATTAAT AT^TAAT Psp1406I 885 AACGTT AA^CGTT PstI 3185 CTGCAG CTGCA^G RcaI 2636 TCATGA T^CATGA SacI 502 GAGCTC GAGCT^C SapI 3046 GAAGAGC GCTCTTC(1/4) ScaI 1974 AGTACT AGT^ACT SmaI 1664 CCCGGG CCC^GGG SspBI 1760 TGTACA T^GTACA Tth111I 1906 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1686 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 4218 TCTAGA T^CTAGA XmaCI 1664 CCCGGG C^CCGGG XmaI 1664 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 1102 GTATAC GT^[AC][GT]AC 2737 GTAGAC Asp700I 2268 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2491 GAATCTTTTC AspHI 502 GAGCTC G[AT]GC[AT]^C 4060 GTGCAC BbsI 426 GTCTTC GAAGAC(2/6) 3920 GTCTTC BpuAI 426 GTCTTC GAAGAC(2/6) 3920 GTCTTC BseRI 1310 GAGGAG GAGGAG(10/8) 1371 GAGGAG BsiHKAI 502 GAGCTC G[AT]GC[AT]^C 4060 GTGCAC BstBI 2951 TTCGAA TT^CGAA 2978 TTCGAA BstEII 1618 GGTAACC G^GT[ACGT]ACC 1899 GGTCACC BstPI 1618 GGTAACC G^GT[ACGT]ACC 1899 GGTCACC CfoI 1683 GCGC GCG^C 2725 GCGC DsaI 2486 CCGTGG C^C[GA][CT]GG 2747 CCACGG EcoO65I 1618 GGTAACC G^GT[ACGT]ACC 1899 GGTCACC EcoRV 2098 GATATC GAT^ATC 3265 GATATC HhaI 1683 GCGC GCG^C 2725 GCGC HinP1I 1683 GCGC G^CGC 2725 GCGC HindIII 1549 AAGCTT A^AGCTT 2974 AAGCTT NspI 2284 GCATGT [GA]CATG^[CT] 3783 ACATGT NspV 2951 TTCGAA TT^CGAA 2978 TTCGAA SexAI 1595 ACCAGGT A^CC[AT]GGT 2336 ACCTGGT SfuI 2951 TTCGAA TT^CGAA 2978 TTCGAA XcmI 1190 CCAATCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1472 CCACTAAATTTATGG XmnI 2268 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2491 GAATCTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AcsI AfaI AflIII AluI AlwI AlwNI ApoI AvaII BanI BbvI BcnI BfaI BmyI BpmI BsaBI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MfeI MnlI MseI MslI MspA1I MspI MunI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I