##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccII AcyI AgeI
Aor51HI ApaI AscI Asp718I AvrII
BamHI BbeI BbvCI BcgI BclI
BglI BglII BlnI BsaHI BsaI
BsiEI BsiWI BspDI BsrBI BsrFI
BssHII BssSI BstAPI BstUI BstYI
Cfr10I ClaI CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoT22I
FauI FbaI FseI HaeII HgaI
Hin1I HpaI KasI KpnI KspI
MflI MluI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmeI PvuI RsrII SacII SalI
SbfI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I StuI SwaI
XhoI XhoII
###################################
# ONE CUT ENZYMES ( 71 enzymes) #
###################################
AccIII 2672 TCCGGA T^CCGGA
AciI 879 GCGG CCGC(-3/-1)
AclI 885 AACGTT AA^CGTT
AflII 30 CTTAAG C^TTAAG
AhdI 1720 GACGACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4060 GTGCAC G^TGCAC
AocI 765 CCTTAGG CC^T[ACGT]AGG
ApaLI 4060 GTGCAC G^TGCAC
AseI 3667 ATTAAT AT^TAAT
AsnI 3667 ATTAAT AT^TAAT
AspEI 1720 GACGACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1906 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1664 CCCGGG C^[CT]CG[GA]G
AviII 2724 TGCGCA TGC^GCA
BalI 2162 TGGCCA TGG^CCA
BanII 502 GAGCTC G[GA]GC[CT]^C
BbrPI 2896 CACGTG CAC^GTG
BciVI 1485 GGATAC GTATCC(6/5)
BfrI 30 CTTAAG C^TTAAG
BlpI 1438 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1438 GCTAAGC GC^T[ACGT]AGC
BsaAI 2896 CACGTG [CT]AC^GT[GA]
BsaWI 2672 TCCGGA [AT]^CCGG[AT]
BseAI 2672 TCCGGA T^CCGGA
BsgI 553 CTGCAC GTGCAG(16/14)
BsmBI 2248 GAGACG CGTCTC(1/5)
BsmI 3015 GAATGC GAATGC(1/-1)
BsoBI 1664 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1760 TGTACA T^GTACA
BspEI 2672 TCCGGA T^CCGGA
BspHI 2636 TCATGA T^CATGA
BspLU11I 3783 ACATGT A^CATGT
BsrDI 2767 GCAATG GCAATG(2/0)
BsrGI 1760 TGTACA T^GTACA
Bst1107I 1102 GTATAC GTA^TAC
BstXI 2747 CCACGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1102 GTATAC GTA^TAC
Bsu36I 765 CCTTAGG CC^T[ACGT]AGG
CelII 1438 GCTAAGC GC^T[ACGT]AGC
DraII 756 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 2162 TGGCCA [CT]^GGCC[GA]
Eam1105I 1720 GACGACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 765 CCTTAGG CC^T[ACGT]AGG
EcoNI 486 CCTTGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 756 AGGACCC [GA]G^G[ACGT]CC[CT]
FspI 2724 TGCGCA TGC^GCA
HincII 2515 GTTGAC GT[CT]^[GA]AC
HindII 2515 GTTGAC GT[CT]^[GA]AC
MluNI 2162 TGGCCA TGG^CCA
MroI 2672 TCCGGA T^CCGGA
MscI 2162 TGGCCA TGG^CCA
PflFI 1906 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1686 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2896 CACGTG CAC^GTG
PmlI 2896 CACGTG CAC^GTG
PpuMI 756 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 2894 GACACGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3667 ATTAAT AT^TAAT
Psp1406I 885 AACGTT AA^CGTT
PstI 3185 CTGCAG CTGCA^G
RcaI 2636 TCATGA T^CATGA
SacI 502 GAGCTC GAGCT^C
SapI 3046 GAAGAGC GCTCTTC(1/4)
ScaI 1974 AGTACT AGT^ACT
SmaI 1664 CCCGGG CCC^GGG
SspBI 1760 TGTACA T^GTACA
Tth111I 1906 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1686 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4218 TCTAGA T^CTAGA
XmaCI 1664 CCCGGG C^CCGGG
XmaI 1664 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1102 GTATAC GT^[AC][GT]AC
2737 GTAGAC
Asp700I 2268 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2491 GAATCTTTTC
AspHI 502 GAGCTC G[AT]GC[AT]^C
4060 GTGCAC
BbsI 426 GTCTTC GAAGAC(2/6)
3920 GTCTTC
BpuAI 426 GTCTTC GAAGAC(2/6)
3920 GTCTTC
BseRI 1310 GAGGAG GAGGAG(10/8)
1371 GAGGAG
BsiHKAI 502 GAGCTC G[AT]GC[AT]^C
4060 GTGCAC
BstBI 2951 TTCGAA TT^CGAA
2978 TTCGAA
BstEII 1618 GGTAACC G^GT[ACGT]ACC
1899 GGTCACC
BstPI 1618 GGTAACC G^GT[ACGT]ACC
1899 GGTCACC
CfoI 1683 GCGC GCG^C
2725 GCGC
DsaI 2486 CCGTGG C^C[GA][CT]GG
2747 CCACGG
EcoO65I 1618 GGTAACC G^GT[ACGT]ACC
1899 GGTCACC
EcoRV 2098 GATATC GAT^ATC
3265 GATATC
HhaI 1683 GCGC GCG^C
2725 GCGC
HinP1I 1683 GCGC G^CGC
2725 GCGC
HindIII 1549 AAGCTT A^AGCTT
2974 AAGCTT
NspI 2284 GCATGT [GA]CATG^[CT]
3783 ACATGT
NspV 2951 TTCGAA TT^CGAA
2978 TTCGAA
SexAI 1595 ACCAGGT A^CC[AT]GGT
2336 ACCTGGT
SfuI 2951 TTCGAA TT^CGAA
2978 TTCGAA
XcmI 1190 CCAATCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1472 CCACTAAATTTATGG
XmnI 2268 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2491 GAATCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
AlwNI ApoI AvaII BanI BbvI
BcnI BfaI BmyI BpmI BsaBI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MfeI MnlI MseI MslI MspA1I
MspI MunI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I