##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AclI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AvrII BbeI BbrPI BglII
BlnI BsaAI BsaBI BseAI BsgI
BsiEI BsiWI BspDI BspEI BssSI
BstAPI BstBI ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I EcoRI
FseI HpaI KasI KpnI MamI
MfeI MluI MroI MunI NarI
NheI NotI NruI NspV PacI
PaeR7I PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SalI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I StuI
XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 895 GTATAC GT^[AC][GT]AC
AcyI 136 GGCGTC G[GA]^CG[CT]C
AgeI 591 ACCGGT A^CCGGT
AhdI 1904 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 255 CCTGAGG CC^T[ACGT]AGG
ApaI 58 GGGCCC GGGCC^C
AspEI 1904 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1254 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1426 TGGCCA TGG^CCA
BamHI 2701 GGATCC G^GATCC
BbvCI 1955 GCTGAGG CCTCAGC(-5/-2)
BcgI 1403 CGAATTGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 2210 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2210 GCTGAGC GC^T[ACGT]AGC
BsaHI 136 GGCGTC G[GA]^CG[CT]C
BsaWI 591 ACCGGT [AT]^CCGG[AT]
BsmBI 272 GAGACG CGTCTC(1/5)
BspHI 2002 TCATGA T^CATGA
Bst1107I 895 GTATAC GTA^TAC
BstEII 769 GGTGACC G^GT[ACGT]ACC
BstPI 769 GGTGACC G^GT[ACGT]ACC
BstXI 2565 CCAACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 895 GTATAC GTA^TAC
Bsu36I 255 CCTGAGG CC^T[ACGT]AGG
CelII 2210 GCTGAGC GC^T[ACGT]AGC
EaeI 1426 TGGCCA [CT]^GGCC[GA]
Eam1105I 1904 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 255 CCTGAGG CC^T[ACGT]AGG
EcoNI 111 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 769 GGTGACC G^GT[ACGT]ACC
EcoT22I 1530 ATGCAT ATGCA^T
HaeII 329 GGCGCT [GA]GCGC^[CT]
Hin1I 136 GGCGTC G[GA]^CG[CT]C
HincII 2459 GTCAAC GT[CT]^[GA]AC
HindII 2459 GTCAAC GT[CT]^[GA]AC
HindIII 4363 AAGCTT A^AGCTT
MluNI 1426 TGGCCA TGG^CCA
MscI 1426 TGGCCA TGG^CCA
NdeI 2403 CATATG CA^TATG
NsiI 1530 ATGCAT ATGCA^T
PflFI 1254 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 591 ACCGGT A^CCGGT
PstI 3089 CTGCAG CTGCA^G
RcaI 2002 TCATGA T^CATGA
SapI 1670 GAAGAGC GCTCTTC(1/4)
SexAI 3463 ACCAGGT A^CC[AT]GGT
SphI 1528 GCATGC GCATG^C
SwaI 4015 ATTTAAAT ATTT^AAAT
Tth111I 1254 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 983 TCTAGA T^CTAGA
XcmI 1606 CCACTATAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AflII 407 CTTAAG C^TTAAG
1823 CTTAAG
AflIII 465 ACATGT A^C[GA][CT]GT
1748 ACATGT
AviII 2284 TGCGCA TGC^GCA
3036 TGCGCA
BanI 3077 GGCACC G^G[CT][GA]CC
4251 GGTGCC
BanII 58 GGGCCC G[GA]GC[CT]^C
1546 GGGCTC
BbsI 412 GTCTTC GAAGAC(2/6)
4279 GTCTTC
BciVI 1744 GGATAC GTATCC(6/5)
2349 GTATCC
BfrI 407 CTTAAG C^TTAAG
1823 CTTAAG
BpuAI 412 GTCTTC GAAGAC(2/6)
4279 GTCTTC
BsaI 1252 GAGACC GGTCTC(1/5)
2683 GAGACC
BsmI 1409 GAATGC GAATGC(1/-1)
3482 GCATTC
BspLU11I 465 ACATGT A^CATGT
1748 ACATGT
BsrBI 660 CCGCTC CCGCTC(-3/-3)
231 GAGCGG
BssHII 183 GCGCGC G^CGCGC
185 GCGCGC
BstYI 1450 GGATCT [GA]^GATC[CT]
2701 GGATCC
EarI 1670 GAAGAG CTCTTC(1/4)
2375 GAAGAG
EcoRV 475 GATATC GAT^ATC
1591 GATATC
FspI 2284 TGCGCA TGC^GCA
3036 TGCGCA
HgaI 246 GACGC GACGC(5/10)
137 GCGTC
Ksp632I 1670 GAAGAG CTCTTC(1/4)
2375 GAAGAG
KspI 310 CCGCGG CCGC^GG
826 CCGCGG
MaeII 1792 ACGT A^CGT
2800 ACGT
MflI 1450 GGATCT [GA]^GATC[CT]
2701 GGATCC
MslI 763 CACAGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3267 CACAACAATG
NaeI 133 GCCGGC GCC^GGC
189 GCCGGC
NcoI 1476 CCATGG C^CATGG
3596 CCATGG
NgoMIV 133 GCCGGC G^CCGGC
189 GCCGGC
PpuMI 259 AGGACCT [GA]G^G[AT]CC[CT]
314 GGGTCCC
SacII 310 CCGCGG CCGC^GG
826 CCGCGG
ScaI 963 AGTACT AGT^ACT
1983 AGTACT
SmaI 61 CCCGGG CCC^GGG
126 CCCGGG
TaqI 54 TCGA T^CGA
1572 TCGA
TthHB8I 54 TCGA T^CGA
1572 TCGA
XhoII 1450 GGATCT [GA]^GATC[CT]
2701 GGATCC
XmaCI 61 CCCGGG C^CCGGG
126 CCCGGG
XmaI 61 CCCGGG C^CCGGG
126 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BbvI BclI BcnI
BfaI BglI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrDI BsrFI BsrGI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XmnI