################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BsoBI BspDI BspLU11I BspMI BsrBI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MaeII MamI MfeI MflI MluI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AlwI 686 GGATC GGATC(4/5) AlwNI 1029 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1064 GGGCCC GGGCC^C AseI 1237 ATTAAT AT^TAAT AsnI 1237 ATTAAT AT^TAAT Asp700I 936 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 784 GGACC G^G[AT]CC BalI 725 TGGCCA TGG^CCA BcgI 211 CGAGTACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 817 GCTCAGC GC^T[ACGT]AGC Bpu1102I 817 GCTCAGC GC^T[ACGT]AGC BsaI 1001 GGTCTC GGTCTC(1/5) BseRI 921 GAGGAG GAGGAG(10/8) BsmI 708 GAATGC GAATGC(1/-1) Bsp1407I 1281 TGTACA T^GTACA BsrGI 1281 TGTACA T^GTACA BssSI 643 CACGAG CACGAG(-5/-1) BstXI 716 CCAAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 817 GCTCAGC GC^T[ACGT]AGC CfoI 108 GCGC GCG^C DraIII 306 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 176 CCATGG C^C[GA][CT]GG EaeI 725 TGGCCA [CT]^GGCC[GA] EarI 115 CTCTTC CTCTTC(1/4) EcoT14I 176 CCATGG C^C[AT][AT]GG HaeII 107 GGCGCT [GA]GCGC^[CT] HhaI 108 GCGC GCG^C HinP1I 108 GCGC G^CGC HindIII 606 AAGCTT A^AGCTT HphI 979 GGTGA GGTGA(8/7) Ksp632I 115 CTCTTC CTCTTC(1/4) MluNI 725 TGGCCA TGG^CCA MscI 725 TGGCCA TGG^CCA MseI 1238 TTAA T^TAA MslI 472 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 176 CCATGG C^CATGG NdeI 761 CATATG CA^TATG PshBI 1237 ATTAAT AT^TAAT PstI 1026 CTGCAG CTGCA^G SacI 203 GAGCTC GAGCT^C ScaI 566 AGTACT AGT^ACT SfaNI 900 GATGC GCATC(5/9) SmlI 559 CTCAAG C^T[CT][GA]AG SphI 1021 GCATGC GCATG^C SspBI 1281 TGTACA T^GTACA StyI 176 CCATGG C^C[AT][AT]GG Tru9I 1238 TTAA T^TAA XcmI 150 CCAGCTGTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 936 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccIII 293 TCCGGA T^CCGGA 781 TCCGGA AspHI 203 GAGCTC G[AT]GC[AT]^C 347 GTGCTC BanII 203 GAGCTC G[GA]GC[CT]^C 1064 GGGCCC BbsI 465 GAAGAC GAAGAC(2/6) 238 GTCTTC BcnI 975 CCGGG CC^[GC]GG 1006 CCCGG BpmI 148 CTCCAG CTGGAG(16/14) 823 CTGGAG BpuAI 465 GAAGAC GAAGAC(2/6) 238 GTCTTC BsaWI 293 TCCGGA [AT]^CCGG[AT] 781 TCCGGA BseAI 293 TCCGGA T^CCGGA 781 TCCGGA BsiHKAI 203 GAGCTC G[AT]GC[AT]^C 347 GTGCTC BspEI 293 TCCGGA T^CCGGA 781 TCCGGA BspHI 665 TCATGA T^CATGA 1042 TCATGA BsrDI 71 CATTGC GCAATG(2/0) 414 GCAATG DraII 1063 AGGGCCC [GA]G^G[ACGT]CC[CT] 1064 GGGCCCC EcoO109I 1063 AGGGCCC [GA]G^G[ACGT]CC[CT] 1064 GGGCCCC EcoT22I 764 ATGCAT ATGCA^T 901 ATGCAT MaeIII 500 GTTAC ^GT[ACGT]AC 1266 GTAAC MroI 293 TCCGGA T^CCGGA 781 TCCGGA NciI 975 CCGGG CC^[GC]GG 1006 CCCGG NsiI 764 ATGCAT ATGCA^T 901 ATGCAT NspI 410 ACATGC [GA]CATG^[CT] 1021 GCATGC PvuII 151 CAGCTG CAG^CTG 820 CAGCTG RcaI 665 TCATGA T^CATGA 1042 TCATGA SfcI 703 CTACAG C^T[GA][CT]AG 1026 CTGCAG Tsp509I 89 AATT ^AATT 1286 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AfaI AluI BbvI BfaI BmyI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeI MboI MboII MnlI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI TaqI TfiI TseI TspRI TthHB8I