##################################
# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BciVI BclI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaHI BsiEI BsiWI BsmBI
BsoBI BspDI BspLU11I BspMI BsrBI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FauI FbaI FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MaeII MamI MfeI
MflI MluI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tsp45I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AlwI 686 GGATC GGATC(4/5)
AlwNI 1029 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1064 GGGCCC GGGCC^C
AseI 1237 ATTAAT AT^TAAT
AsnI 1237 ATTAAT AT^TAAT
Asp700I 936 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 784 GGACC G^G[AT]CC
BalI 725 TGGCCA TGG^CCA
BcgI 211 CGAGTACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 817 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 817 GCTCAGC GC^T[ACGT]AGC
BsaI 1001 GGTCTC GGTCTC(1/5)
BseRI 921 GAGGAG GAGGAG(10/8)
BsmI 708 GAATGC GAATGC(1/-1)
Bsp1407I 1281 TGTACA T^GTACA
BsrGI 1281 TGTACA T^GTACA
BssSI 643 CACGAG CACGAG(-5/-1)
BstXI 716 CCAAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 817 GCTCAGC GC^T[ACGT]AGC
CfoI 108 GCGC GCG^C
DraIII 306 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 176 CCATGG C^C[GA][CT]GG
EaeI 725 TGGCCA [CT]^GGCC[GA]
EarI 115 CTCTTC CTCTTC(1/4)
EcoT14I 176 CCATGG C^C[AT][AT]GG
HaeII 107 GGCGCT [GA]GCGC^[CT]
HhaI 108 GCGC GCG^C
HinP1I 108 GCGC G^CGC
HindIII 606 AAGCTT A^AGCTT
HphI 979 GGTGA GGTGA(8/7)
Ksp632I 115 CTCTTC CTCTTC(1/4)
MluNI 725 TGGCCA TGG^CCA
MscI 725 TGGCCA TGG^CCA
MseI 1238 TTAA T^TAA
MslI 472 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 176 CCATGG C^CATGG
NdeI 761 CATATG CA^TATG
PshBI 1237 ATTAAT AT^TAAT
PstI 1026 CTGCAG CTGCA^G
SacI 203 GAGCTC GAGCT^C
ScaI 566 AGTACT AGT^ACT
SfaNI 900 GATGC GCATC(5/9)
SmlI 559 CTCAAG C^T[CT][GA]AG
SphI 1021 GCATGC GCATG^C
SspBI 1281 TGTACA T^GTACA
StyI 176 CCATGG C^C[AT][AT]GG
Tru9I 1238 TTAA T^TAA
XcmI 150 CCAGCTGTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 936 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 293 TCCGGA T^CCGGA
781 TCCGGA
AspHI 203 GAGCTC G[AT]GC[AT]^C
347 GTGCTC
BanII 203 GAGCTC G[GA]GC[CT]^C
1064 GGGCCC
BbsI 465 GAAGAC GAAGAC(2/6)
238 GTCTTC
BcnI 975 CCGGG CC^[GC]GG
1006 CCCGG
BpmI 148 CTCCAG CTGGAG(16/14)
823 CTGGAG
BpuAI 465 GAAGAC GAAGAC(2/6)
238 GTCTTC
BsaWI 293 TCCGGA [AT]^CCGG[AT]
781 TCCGGA
BseAI 293 TCCGGA T^CCGGA
781 TCCGGA
BsiHKAI 203 GAGCTC G[AT]GC[AT]^C
347 GTGCTC
BspEI 293 TCCGGA T^CCGGA
781 TCCGGA
BspHI 665 TCATGA T^CATGA
1042 TCATGA
BsrDI 71 CATTGC GCAATG(2/0)
414 GCAATG
DraII 1063 AGGGCCC [GA]G^G[ACGT]CC[CT]
1064 GGGCCCC
EcoO109I 1063 AGGGCCC [GA]G^G[ACGT]CC[CT]
1064 GGGCCCC
EcoT22I 764 ATGCAT ATGCA^T
901 ATGCAT
MaeIII 500 GTTAC ^GT[ACGT]AC
1266 GTAAC
MroI 293 TCCGGA T^CCGGA
781 TCCGGA
NciI 975 CCGGG CC^[GC]GG
1006 CCCGG
NsiI 764 ATGCAT ATGCA^T
901 ATGCAT
NspI 410 ACATGC [GA]CATG^[CT]
1021 GCATGC
PvuII 151 CAGCTG CAG^CTG
820 CAGCTG
RcaI 665 TCATGA T^CATGA
1042 TCATGA
SfcI 703 CTACAG C^T[GA][CT]AG
1026 CTGCAG
Tsp509I 89 AATT ^AATT
1286 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI BbvI BfaI
BmyI BsaJI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MaeI MboI MboII MnlI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI TaqI TfiI TseI TspRI
TthHB8I