##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccI AccII AclI AflII
AflIII AlwNI AocI ApaI AscI
AspI AvaI AviII AvrII BbrPI
BbvCI BcgI BfrI BglI BglII
BlnI BsaBI BsiEI BsiWI BsoBI
BspDI BspLU11I BsrBI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco52I
Eco81I EcoNI EcoO65I FauI FseI
FspI HindIII HpaI KspI MamI
MluI MvnI NaeI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SfiI
SgrAI SmaI SpeI Sse8387I SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 741 GGTACC G^GTACC
AccIII 927 TCCGGA T^CCGGA
AgeI 702 ACCGGT A^CCGGT
AhdI 1780 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3762 GTGCAC G^TGCAC
Aor51HI 3683 AGCGCT AGC^GCT
ApaLI 3762 GTGCAC G^TGCAC
Asp700I 461 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 741 GGTACC G^GTACC
AspEI 1780 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2723 TGGCCA TGG^CCA
BamHI 382 GGATCC G^GATCC
BanII 758 GGGCTC G[GA]GC[CT]^C
BclI 3010 TGATCA T^GATCA
BcnI 166 CCGGG CC^[GC]GG
BlpI 3148 GCTCAGC GC^T[ACGT]AGC
BpmI 686 CTCCAG CTGGAG(16/14)
Bpu1102I 3148 GCTCAGC GC^T[ACGT]AGC
BsaAI 4037 TACGTA [CT]AC^GT[GA]
BsaI 2378 GGTCTC GGTCTC(1/5)
BseAI 927 TCCGGA T^CCGGA
BsmBI 1029 GAGACG CGTCTC(1/5)
Bsp1407I 2041 TGTACA T^GTACA
BspEI 927 TCCGGA T^CCGGA
BspHI 3909 TCATGA T^CATGA
BsrGI 2041 TGTACA T^GTACA
BstBI 420 TTCGAA TT^CGAA
CelII 3148 GCTCAGC GC^T[ACGT]AGC
DraII 630 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 1780 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3683 AGCGCT AGC^GCT
EcoO109I 630 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 530 GAATTC G^AATTC
EcoRV 3321 GATATC GAT^ATC
FbaI 3010 TGATCA T^GATCA
HincII 1336 GTTGAC GT[CT]^[GA]AC
HindII 1336 GTTGAC GT[CT]^[GA]AC
KpnI 741 GGTACC GGTAC^C
MluNI 2723 TGGCCA TGG^CCA
MroI 927 TCCGGA T^CCGGA
MscI 2723 TGGCCA TGG^CCA
NciI 166 CCGGG CC^[GC]GG
NcoI 8 CCATGG C^CATGG
NspV 420 TTCGAA TT^CGAA
PinAI 702 ACCGGT A^CCGGT
PvuII 607 CAGCTG CAG^CTG
RcaI 3909 TCATGA T^CATGA
SexAI 432 ACCTGGT A^CC[AT]GGT
SfuI 420 TTCGAA TT^CGAA
SmlI 1331 CTCAAG C^T[CT][GA]AG
SnaBI 4037 TACGTA TAC^GTA
SphI 1695 GCATGC GCATG^C
SspBI 2041 TGTACA T^GTACA
StuI 134 AGGCCT AGG^CCT
XmnI 461 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BbeI 43 GGCGCC GGCGC^C
2311 GGCGCC
BciVI 544 GGATAC GTATCC(6/5)
2069 GGATAC
BsaWI 702 ACCGGT [AT]^CCGG[AT]
927 TCCGGA
BsgI 3043 CTGCAC GTGCAG(16/14)
718 GTGCAG
BsmI 399 GAATGC GAATGC(1/-1)
4017 GAATGC
BspMI 744 ACCTGC ACCTGC(4/8)
738 GCAGGT
BsrDI 594 CATTGC GCAATG(2/0)
3070 CATTGC
BsrFI 702 ACCGGT [GA]^CCGG[CT]
726 ACCGGC
Cfr10I 702 ACCGGT [GA]^CCGG[CT]
726 ACCGGC
DsaI 8 CCATGG C^C[GA][CT]GG
92 CCACGG
EcoT22I 401 ATGCAT ATGCA^T
4163 ATGCAT
KasI 43 GGCGCC G^GCGCC
2311 GGCGCC
MslI 1539 CACAGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1690 CATCTGCATG
MspA1I 36 CAGCGG C[AC]G^C[GT]G
607 CAGCTG
NarI 43 GGCGCC GG^CGCC
2311 GGCGCC
NsiI 401 ATGCAT ATGCA^T
4163 ATGCAT
NspI 1695 GCATGC [GA]CATG^[CT]
3345 GCATGT
PflMI 649 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1576 CCAAGTATTGG
PstI 317 CTGCAG CTGCA^G
610 CTGCAG
SapI 1888 GAAGAGC GCTCTTC(1/4)
2905 GCTCTTC
SfoI 43 GGCGCC GGC^GCC
2311 GGCGCC
Van91I 649 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1576 CCAAGTATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AseI AsnI AspHI
AvaII BanI BbsI BbvI BfaI
BmyI BpuAI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MspI
MunI MvaI MwoI NdeII NlaIII
NlaIV PleI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII