##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BanI BanII BbeI BbrPI BbvCI
BcgI BcnI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI DsaI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRV FauI
FseI FspI HaeII HhaI Hin1I
HinP1I HindIII KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI MwoI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
StuI Tth111I Van91I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 1468 GTATAC GT^[AC][GT]AC
AflII 3955 CTTAAG C^TTAAG
AlwNI 3970 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 1518 TGGCCA TGG^CCA
BamHI 2763 GGATCC G^GATCC
BciVI 3233 GTATCC GTATCC(6/5)
BclI 2968 TGATCA T^GATCA
BfrI 3955 CTTAAG C^TTAAG
BsaAI 434 TACGTG [CT]AC^GT[GA]
BseRI 170 GAGGAG GAGGAG(10/8)
BsgI 3088 GTGCAG GTGCAG(16/14)
BsiEI 18 CGGCCG CG[GA][CT]^CG
BsmFI 697 GGGAC GGGAC(10/14)
BsmI 2136 GAATGC GAATGC(1/-1)
BspMI 2343 ACCTGC ACCTGC(4/8)
BsrDI 3373 GCAATG GCAATG(2/0)
Bst1107I 1468 GTATAC GTA^TAC
BstXI 2151 CCAAGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2763 GGATCC [GA]^GATC[CT]
BstZ17I 1468 GTATAC GTA^TAC
Cac8I 1424 GCTTGC GC[ACGT]^[ACGT]GC
DraII 2315 AGGTCCT [GA]G^G[ACGT]CC[CT]
EagI 18 CGGCCG C^GGCCG
EclXI 18 CGGCCG C^GGCCG
Eco52I 18 CGGCCG C^GGCCG
EcoO109I 2315 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 966 GAATTC G^AATTC
EcoT14I 386 CCAAGG C^C[AT][AT]GG
EcoT22I 2118 ATGCAT ATGCA^T
FbaI 2968 TGATCA T^GATCA
HapII 21 CCGG C^CGG
HgaI 2989 GACGC GACGC(5/10)
HpaII 21 CCGG C^CGG
MflI 2763 GGATCC [GA]^GATC[CT]
MluNI 1518 TGGCCA TGG^CCA
MscI 1518 TGGCCA TGG^CCA
MspI 21 CCGG C^CGG
NsiI 2118 ATGCAT ATGCA^T
PpuMI 2315 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 572 CTGCAG CTGCA^G
PvuII 374 CAGCTG CAG^CTG
StyI 386 CCAAGG C^C[AT][AT]GG
SwaI 1808 ATTTAAAT ATTT^AAAT
XhoII 2763 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 2142 ACATGT A^C[GA][CT]GT
2446 ACATGT
AspHI 2535 GTGCTC G[AT]GC[AT]^C
3716 GTGCTC
BbsI 412 GAAGAC GAAGAC(2/6)
2778 GTCTTC
BmyI 2535 GTGCTC G[AGT]GC[ACT]^C
3716 GTGCTC
BpuAI 412 GAAGAC GAAGAC(2/6)
2778 GTCTTC
BsiHKAI 2535 GTGCTC G[AT]GC[AT]^C
3716 GTGCTC
BsiYI 658 CCTTATATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3265 CCAGTTAGGGG
BslI 658 CCTTATATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3265 CCAGTTAGGGG
Bsp1286I 2535 GTGCTC G[AGT]GC[ACT]^C
3716 GTGCTC
BspLU11I 2142 ACATGT A^CATGT
2446 ACATGT
EaeI 18 CGGCCG [CT]^GGCC[GA]
1518 TGGCCA
EarI 272 GAAGAG CTCTTC(1/4)
2052 GAAGAG
HpaI 1607 GTTAAC GTT^AAC
4051 GTTAAC
Ksp632I 272 GAAGAG CTCTTC(1/4)
2052 GAAGAG
MaeII 435 ACGT A^CGT
2352 ACGT
MspA1I 11 CCGCTG C[AC]G^C[GT]G
374 CAGCTG
NdeI 1834 CATATG CA^TATG
3388 CATATG
NlaIV 1340 GGGTCC GG[ACGT]^[ACGT]CC
2763 GGATCC
SfcI 572 CTGCAG C^T[GA][CT]AG
2130 CTGTAG
SpeI 2233 ACTAGT A^CTAGT
3397 ACTAGT
TaqI 229 TCGA T^CGA
1449 TCGA
TthHB8I 229 TCGA T^CGA
1449 TCGA
XcmI 2503 CCATCTGTCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3285 CCAAATTAAATTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI Asp700I AvaII
BbvI BfaI BpmI BsaJI BsmAI
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII DraI EcoRII Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MslI MvaI
NdeII NlaIII NspI PleI PshBI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI SspI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XbaI XmnI