################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BanI BanII BbeI BbrPI BbvCI BcgI BcnI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRV FauI FseI FspI HaeII HhaI Hin1I HinP1I HindIII KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI MwoI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 1468 GTATAC GT^[AC][GT]AC AflII 3955 CTTAAG C^TTAAG AlwNI 3970 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG BalI 1518 TGGCCA TGG^CCA BamHI 2763 GGATCC G^GATCC BciVI 3233 GTATCC GTATCC(6/5) BclI 2968 TGATCA T^GATCA BfrI 3955 CTTAAG C^TTAAG BsaAI 434 TACGTG [CT]AC^GT[GA] BseRI 170 GAGGAG GAGGAG(10/8) BsgI 3088 GTGCAG GTGCAG(16/14) BsiEI 18 CGGCCG CG[GA][CT]^CG BsmFI 697 GGGAC GGGAC(10/14) BsmI 2136 GAATGC GAATGC(1/-1) BspMI 2343 ACCTGC ACCTGC(4/8) BsrDI 3373 GCAATG GCAATG(2/0) Bst1107I 1468 GTATAC GTA^TAC BstXI 2151 CCAAGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 2763 GGATCC [GA]^GATC[CT] BstZ17I 1468 GTATAC GTA^TAC Cac8I 1424 GCTTGC GC[ACGT]^[ACGT]GC DraII 2315 AGGTCCT [GA]G^G[ACGT]CC[CT] EagI 18 CGGCCG C^GGCCG EclXI 18 CGGCCG C^GGCCG Eco52I 18 CGGCCG C^GGCCG EcoO109I 2315 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 966 GAATTC G^AATTC EcoT14I 386 CCAAGG C^C[AT][AT]GG EcoT22I 2118 ATGCAT ATGCA^T FbaI 2968 TGATCA T^GATCA HapII 21 CCGG C^CGG HgaI 2989 GACGC GACGC(5/10) HpaII 21 CCGG C^CGG MflI 2763 GGATCC [GA]^GATC[CT] MluNI 1518 TGGCCA TGG^CCA MscI 1518 TGGCCA TGG^CCA MspI 21 CCGG C^CGG NsiI 2118 ATGCAT ATGCA^T PpuMI 2315 AGGTCCT [GA]G^G[AT]CC[CT] PstI 572 CTGCAG CTGCA^G PvuII 374 CAGCTG CAG^CTG StyI 386 CCAAGG C^C[AT][AT]GG SwaI 1808 ATTTAAAT ATTT^AAAT XhoII 2763 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AflIII 2142 ACATGT A^C[GA][CT]GT 2446 ACATGT AspHI 2535 GTGCTC G[AT]GC[AT]^C 3716 GTGCTC BbsI 412 GAAGAC GAAGAC(2/6) 2778 GTCTTC BmyI 2535 GTGCTC G[AGT]GC[ACT]^C 3716 GTGCTC BpuAI 412 GAAGAC GAAGAC(2/6) 2778 GTCTTC BsiHKAI 2535 GTGCTC G[AT]GC[AT]^C 3716 GTGCTC BsiYI 658 CCTTATATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 3265 CCAGTTAGGGG BslI 658 CCTTATATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 3265 CCAGTTAGGGG Bsp1286I 2535 GTGCTC G[AGT]GC[ACT]^C 3716 GTGCTC BspLU11I 2142 ACATGT A^CATGT 2446 ACATGT EaeI 18 CGGCCG [CT]^GGCC[GA] 1518 TGGCCA EarI 272 GAAGAG CTCTTC(1/4) 2052 GAAGAG HpaI 1607 GTTAAC GTT^AAC 4051 GTTAAC Ksp632I 272 GAAGAG CTCTTC(1/4) 2052 GAAGAG MaeII 435 ACGT A^CGT 2352 ACGT MspA1I 11 CCGCTG C[AC]G^C[GT]G 374 CAGCTG NdeI 1834 CATATG CA^TATG 3388 CATATG NlaIV 1340 GGGTCC GG[ACGT]^[ACGT]CC 2763 GGATCC SfcI 572 CTGCAG C^T[GA][CT]AG 2130 CTGTAG SpeI 2233 ACTAGT A^CTAGT 3397 ACTAGT TaqI 229 TCGA T^CGA 1449 TCGA TthHB8I 229 TCGA T^CGA 1449 TCGA XcmI 2503 CCATCTGTCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3285 CCAAATTAAATTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AseI AsnI Asp700I AvaII BbvI BfaI BpmI BsaJI BsmAI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII DraI EcoRII Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MvaI NdeII NlaIII NspI PleI PshBI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI SspI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XbaI XmnI