##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbrPI BbvCI BfrI
BglI BlnI BsaBI BseAI BsiWI
BspEI BspHI BstAPI BstBI Bsu36I
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRV EcoT14I
EcoT22I FseI FspI KpnI MamI
MfeI MroI MunI NcoI NotI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
RcaI RsrII SacI SalI SbfI
ScaI SexAI SfiI SfuI SnaBI
SpeI Sse8387I StyI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 3606 GACGTC GACGT^C
BamHI 3276 GGATCC G^GATCC
BcgI 1191 GCACCGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2618 GTATCC GTATCC(6/5)
BglII 3691 AGATCT A^GATCT
BlpI 607 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 607 GCTCAGC GC^T[ACGT]AGC
BsaAI 1613 TACGTG [CT]AC^GT[GA]
BsiEI 364 CGATCG CG[GA][CT]^CG
BsmBI 3397 GAGACG CGTCTC(1/5)
BspDI 362 ATCGAT AT^CGAT
BspLU11I 3711 ACATGT A^CATGT
Bst1107I 830 GTATAC GTA^TAC
BstEII 1366 GGTGACC G^GT[ACGT]ACC
BstPI 1366 GGTGACC G^GT[ACGT]ACC
BstZ17I 830 GTATAC GTA^TAC
CelII 607 GCTCAGC GC^T[ACGT]AGC
ClaI 362 ATCGAT AT^CGAT
DrdI 3584 GACCCTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 591 CTCTTC CTCTTC(1/4)
EcoO65I 1366 GGTGACC G^GT[ACGT]ACC
HpaI 3141 GTTAAC GTT^AAC
Ksp632I 591 CTCTTC CTCTTC(1/4)
KspI 3367 CCGCGG CCGC^GG
MluI 1905 ACGCGT A^CGCGT
NdeI 4360 CATATG CA^TATG
NheI 4261 GCTAGC G^CTAGC
PaeR7I 1797 CTCGAG C^TCGAG
PflMI 3384 CCAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1993 GACTCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 364 CGATCG CGAT^CG
PvuII 2689 CAGCTG CAG^CTG
SacII 3367 CCGCGG CCGC^GG
SapI 590 GCTCTTC GCTCTTC(1/4)
SgrAI 1779 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 126 CCCGGG CCC^GGG
SphI 2538 GCATGC GCATG^C
StuI 2416 AGGCCT AGG^CCT
SwaI 3948 ATTTAAAT ATTT^AAAT
Van91I 3384 CCAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1797 CTCGAG C^TCGAG
XmaCI 126 CCCGGG C^CCGGG
XmaI 126 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 830 GTATAC GT^[AC][GT]AC
3938 GTCTAC
AlwNI 242 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1655 CAGCCCCTG
ApaI 2155 GGGCCC GGGCC^C
2380 GGGCCC
BalI 2609 TGGCCA TGG^CCA
3344 TGGCCA
BbeI 1792 GGCGCC GGCGC^C
2116 GGCGCC
BbsI 488 GTCTTC GAAGAC(2/6)
2128 GTCTTC
BpuAI 488 GTCTTC GAAGAC(2/6)
2128 GTCTTC
BsaWI 751 ACCGGA [AT]^CCGG[AT]
2729 TCCGGT
BsgI 397 GTGCAG GTGCAG(16/14)
1818 GTGCAG
BsmI 3899 GAATGC GAATGC(1/-1)
2571 GCATTC
BsrDI 3959 CATTGC GCAATG(2/0)
92 GCAATG
BstXI 2217 CCAGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2319 CCAGCGCGTTGG
HindIII 3122 AAGCTT A^AGCTT
4458 AAGCTT
KasI 1792 GGCGCC G^GCGCC
2116 GGCGCC
MluNI 2609 TGGCCA TGG^CCA
3344 TGGCCA
MscI 2609 TGGCCA TGG^CCA
3344 TGGCCA
NarI 1792 GGCGCC GG^CGCC
2116 GGCGCC
NruI 367 TCGCGA TCG^CGA
1336 TCGCGA
SfoI 1792 GGCGCC GGC^GCC
2116 GGCGCC
SmlI 1797 CTCGAG C^T[CT][GA]AG
3523 CTCAAG
TfiI 524 GAATC G^A[AT]TC
980 GATTC
XbaI 527 TCTAGA T^CTAGA
3091 TCTAGA
XcmI 1410 CCAGGGTGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3240 CCAGTGAGAGGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI ApaLI
ApoI AspHI AvaI AvaII BanI
BanII BbvI BclI BcnI BfaI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrFI BsrGI BsrI BssHII
BssKI BssSI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EcoNI EcoO109I EcoRI EcoRII
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI SspI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII