##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AclI AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BamHI
BbvCI BfrI BlnI BsiWI BsmI
BspLU11I BssHII Bst1107I BstBI BstZ17I
CpoI Eco47III EcoRI EcoRV FseI
FspI HindIII HpaI KpnI MfeI
MluI MunI NdeI NheI NotI
NruI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RsrII SalI
SapI SbfI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 1123 GTCTAC GT^[AC][GT]AC
AccIII 1102 TCCGGA T^CCGGA
AcsI 3593 GAATTT [GA]^AATT[CT]
AflIII 242 ACGTGT A^C[GA][CT]GT
AhdI 2881 GACGTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3729 CCTGAGG CC^T[ACGT]AGG
ApaI 12 GGGCCC GGGCC^C
ApoI 3593 GAATTT [GA]^AATT[CT]
AspEI 2881 GACGTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1117 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 2141 TGATCA T^GATCA
BlpI 145 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 145 GCTGAGC GC^T[ACGT]AGC
BseAI 1102 TCCGGA T^CCGGA
BsiEI 1697 CGGCCG CG[GA][CT]^CG
BsmBI 3161 GAGACG CGTCTC(1/5)
Bsp1407I 1188 TGTACA T^GTACA
BspDI 1843 ATCGAT AT^CGAT
BspEI 1102 TCCGGA T^CCGGA
BspHI 521 TCATGA T^CATGA
BsrBI 2524 GAGCGG CCGCTC(-3/-3)
BsrGI 1188 TGTACA T^GTACA
BssSI 3158 CACGAG CACGAG(-5/-1)
Bsu36I 3729 CCTGAGG CC^T[ACGT]AGG
CelII 145 GCTGAGC GC^T[ACGT]AGC
ClaI 1843 ATCGAT AT^CGAT
DraI 3265 TTTAAA TTT^AAA
DraIII 3940 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2013 GACGCTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1697 CGGCCG C^GGCCG
Eam1105I 2881 GACGTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1697 CGGCCG C^GGCCG
Eco52I 1697 CGGCCG C^GGCCG
Eco81I 3729 CCTGAGG CC^T[ACGT]AGG
EcoT22I 1378 ATGCAT ATGCA^T
FbaI 2141 TGATCA T^GATCA
MroI 1102 TCCGGA T^CCGGA
NsiI 1378 ATGCAT ATGCA^T
PaeR7I 3829 CTCGAG C^TCGAG
PflFI 1117 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 521 TCATGA T^CATGA
SacI 1483 GAGCTC GAGCT^C
SspBI 1188 TGTACA T^GTACA
SspI 3258 AATATT AAT^ATT
Tth111I 1117 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2364 TCTAGA T^CTAGA
XhoI 3829 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 2881 GACGTC GACGT^C
3163 GACGTC
BbrPI 313 CACGTG CAC^GTG
3067 CACGTG
BciVI 468 GGATAC GTATCC(6/5)
2963 GTATCC
BglII 1835 AGATCT A^GATCT
1994 AGATCT
BsaI 379 GAGACC GGTCTC(1/5)
1063 GAGACC
BsaWI 1102 TCCGGA [AT]^CCGG[AT]
2453 ACCGGA
BstEII 1530 GGTCACC G^GT[ACGT]ACC
3390 GGTGACC
BstPI 1530 GGTCACC G^GT[ACGT]ACC
3390 GGTGACC
EcoO65I 1530 GGTCACC G^GT[ACGT]ACC
3390 GGTGACC
HgaI 2013 GACGC GACGC(5/10)
518 GCGTC
HincII 2314 GTCAAC GT[CT]^[GA]AC
2683 GTCAAC
HindII 2314 GTCAAC GT[CT]^[GA]AC
2683 GTCAAC
KspI 17 CCGCGG CCGC^GG
25 CCGCGG
PflMI 2217 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2420 CCATCCGCTGG
PmaCI 313 CACGTG CAC^GTG
3067 CACGTG
PmlI 313 CACGTG CAC^GTG
3067 CACGTG
PshAI 554 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3850 GACTGCCGTC
SacII 17 CCGCGG CCGC^GG
25 CCGCGG
ScaI 1469 AGTACT AGT^ACT
4393 AGTACT
SexAI 230 ACCAGGT A^CC[AT]GGT
2324 ACCTGGT
SfiI 4 GGCCGTCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4212 GGCCATCTGGGCC
SmaI 39 CCCGGG CCC^GGG
719 CCCGGG
SmlI 2017 CTCAAG C^T[CT][GA]AG
3829 CTCGAG
SphI 2228 GCATGC GCATG^C
4560 GCATGC
Van91I 2217 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2420 CCATCCGCTGG
XcmI 4223 CCATTCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4622 CCAGACCTTTGCTGG
XmaCI 39 CCCGGG C^CCGGG
719 CCCGGG
XmaI 39 CCCGGG C^CCGGG
719 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvI BcgI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaBI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII