##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
BamHI BbrPI BcgI BclI BfrI
BsiWI BspDI BsrBI BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI KpnI
KspI MfeI MluI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SfiI SfuI SmaI
SpeI Sse8387I StuI SwaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 3044 TCCGGA T^CCGGA
AcyI 197 GGCGCC G[GA]^CG[CT]C
AflIII 2800 ACATGT A^C[GA][CT]GT
Alw44I 2154 GTGCAC G^TGCAC
ApaI 2841 GGGCCC GGGCC^C
ApaLI 2154 GTGCAC G^TGCAC
BbeI 197 GGCGCC GGCGC^C
BciVI 4412 GTATCC GTATCC(6/5)
BglI 3914 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3256 AGATCT A^GATCT
BlpI 1010 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1010 GCTCAGC GC^T[ACGT]AGC
BsaAI 3729 TACGTA [CT]AC^GT[GA]
BsaBI 2982 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 197 GGCGCC G[GA]^CG[CT]C
BsaWI 3044 TCCGGA [AT]^CCGG[AT]
BseAI 3044 TCCGGA T^CCGGA
BsiEI 2327 CGACCG CG[GA][CT]^CG
BsmBI 1040 GAGACG CGTCTC(1/5)
BsmI 3858 GCATTC GAATGC(1/-1)
BspEI 3044 TCCGGA T^CCGGA
BspHI 3581 TCATGA T^CATGA
BspLU11I 2800 ACATGT A^CATGT
BsrDI 309 GCAATG GCAATG(2/0)
BstAPI 1100 GCAATCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 549 GGTAACC G^GT[ACGT]ACC
BstPI 549 GGTAACC G^GT[ACGT]ACC
BstYI 3256 AGATCT [GA]^GATC[CT]
CelII 1010 GCTCAGC GC^T[ACGT]AGC
DraIII 1538 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 549 GGTAACC G^GT[ACGT]ACC
HgaI 682 GCGTC GACGC(5/10)
Hin1I 197 GGCGCC G[GA]^CG[CT]C
HindIII 1630 AAGCTT A^AGCTT
KasI 197 GGCGCC G^GCGCC
MamI 2982 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 3256 AGATCT [GA]^GATC[CT]
MroI 3044 TCCGGA T^CCGGA
NaeI 88 GCCGGC GCC^GGC
NarI 197 GGCGCC GG^CGCC
NgoMIV 88 GCCGGC G^CCGGC
RcaI 3581 TCATGA T^CATGA
SexAI 2767 ACCAGGT A^CC[AT]GGT
SfoI 197 GGCGCC GGC^GCC
SgrAI 277 CACCGGCG C[GA]^CCGG[CT]G
SnaBI 3729 TACGTA TAC^GTA
SphI 678 GCATGC GCATG^C
SspI 4098 AATATT AAT^ATT
XhoII 3256 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AocI 702 CCTAAGG CC^T[ACGT]AGG
4056 CCTCAGG
Bsp1407I 447 TGTACA T^GTACA
4233 TGTACA
BsrGI 447 TGTACA T^GTACA
4233 TGTACA
Bsu36I 702 CCTAAGG CC^T[ACGT]AGG
4056 CCTCAGG
DsaI 201 CCGTGG C^C[GA][CT]GG
234 CCGTGG
Eco81I 702 CCTAAGG CC^T[ACGT]AGG
4056 CCTCAGG
EcoNI 40 CCTTCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1740 CCTGTGACAGG
HaeII 69 GGCGCT [GA]GCGC^[CT]
197 GGCGCC
MaeII 2691 ACGT A^CGT
3730 ACGT
PflMI 802 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3622 CCACAGTTTGG
PpuMI 141 GGGTCCC [GA]G^G[AT]CC[CT]
3959 AGGTCCT
SacI 239 GAGCTC GAGCT^C
3214 GAGCTC
SspBI 447 TGTACA T^GTACA
4233 TGTACA
TaqI 1238 TCGA T^CGA
1863 TCGA
TthHB8I 1238 TCGA T^CGA
1863 TCGA
Van91I 802 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3622 CCACAGTTTGG
XcmI 290 CCACCTCACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3542 CCATTAGACCAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AspI
AvaI AvaII AvrII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BlnI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I XbaI