################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII Acc65I AccI AclI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AviII BamHI BbrPI BcgI BclI BfrI BsiWI BspDI BsrBI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KpnI KspI MfeI MluI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfuI SmaI SpeI Sse8387I StuI SwaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccIII 3044 TCCGGA T^CCGGA AcyI 197 GGCGCC G[GA]^CG[CT]C AflIII 2800 ACATGT A^C[GA][CT]GT Alw44I 2154 GTGCAC G^TGCAC ApaI 2841 GGGCCC GGGCC^C ApaLI 2154 GTGCAC G^TGCAC BbeI 197 GGCGCC GGCGC^C BciVI 4412 GTATCC GTATCC(6/5) BglI 3914 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3256 AGATCT A^GATCT BlpI 1010 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1010 GCTCAGC GC^T[ACGT]AGC BsaAI 3729 TACGTA [CT]AC^GT[GA] BsaBI 2982 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 197 GGCGCC G[GA]^CG[CT]C BsaWI 3044 TCCGGA [AT]^CCGG[AT] BseAI 3044 TCCGGA T^CCGGA BsiEI 2327 CGACCG CG[GA][CT]^CG BsmBI 1040 GAGACG CGTCTC(1/5) BsmI 3858 GCATTC GAATGC(1/-1) BspEI 3044 TCCGGA T^CCGGA BspHI 3581 TCATGA T^CATGA BspLU11I 2800 ACATGT A^CATGT BsrDI 309 GCAATG GCAATG(2/0) BstAPI 1100 GCAATCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 549 GGTAACC G^GT[ACGT]ACC BstPI 549 GGTAACC G^GT[ACGT]ACC BstYI 3256 AGATCT [GA]^GATC[CT] CelII 1010 GCTCAGC GC^T[ACGT]AGC DraIII 1538 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 549 GGTAACC G^GT[ACGT]ACC HgaI 682 GCGTC GACGC(5/10) Hin1I 197 GGCGCC G[GA]^CG[CT]C HindIII 1630 AAGCTT A^AGCTT KasI 197 GGCGCC G^GCGCC MamI 2982 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 3256 AGATCT [GA]^GATC[CT] MroI 3044 TCCGGA T^CCGGA NaeI 88 GCCGGC GCC^GGC NarI 197 GGCGCC GG^CGCC NgoMIV 88 GCCGGC G^CCGGC RcaI 3581 TCATGA T^CATGA SexAI 2767 ACCAGGT A^CC[AT]GGT SfoI 197 GGCGCC GGC^GCC SgrAI 277 CACCGGCG C[GA]^CCGG[CT]G SnaBI 3729 TACGTA TAC^GTA SphI 678 GCATGC GCATG^C SspI 4098 AATATT AAT^ATT XhoII 3256 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AocI 702 CCTAAGG CC^T[ACGT]AGG 4056 CCTCAGG Bsp1407I 447 TGTACA T^GTACA 4233 TGTACA BsrGI 447 TGTACA T^GTACA 4233 TGTACA Bsu36I 702 CCTAAGG CC^T[ACGT]AGG 4056 CCTCAGG DsaI 201 CCGTGG C^C[GA][CT]GG 234 CCGTGG Eco81I 702 CCTAAGG CC^T[ACGT]AGG 4056 CCTCAGG EcoNI 40 CCTTCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1740 CCTGTGACAGG HaeII 69 GGCGCT [GA]GCGC^[CT] 197 GGCGCC MaeII 2691 ACGT A^CGT 3730 ACGT PflMI 802 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3622 CCACAGTTTGG PpuMI 141 GGGTCCC [GA]G^G[AT]CC[CT] 3959 AGGTCCT SacI 239 GAGCTC GAGCT^C 3214 GAGCTC SspBI 447 TGTACA T^GTACA 4233 TGTACA TaqI 1238 TCGA T^CGA 1863 TCGA TthHB8I 1238 TCGA T^CGA 1863 TCGA Van91I 802 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3622 CCACAGTTTGG XcmI 290 CCACCTCACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3542 CCATTAGACCAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AspI AvaI AvaII AvrII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BlnI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I XbaI