##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspI
AviII BbrPI BcgI BsiEI BsiWI
BspDI BsrBI BsrFI BssSI BstBI
Bsu36I Cfr10I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRV FseI FspI HpaI
KpnI KspI MluI NaeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I StuI SwaI Tth111I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 751 TCCGGA T^CCGGA
AcyI 274 GGCGCC G[GA]^CG[CT]C
AflII 2643 CTTAAG C^TTAAG
AlwNI 1411 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 4311 ATTAAT AT^TAAT
AsnI 4311 ATTAAT AT^TAAT
AspHI 1302 GTGCTC G[AT]GC[AT]^C
AvrII 3499 CCTAGG C^CTAGG
BbeI 274 GGCGCC GGCGC^C
BbsI 2169 GAAGAC GAAGAC(2/6)
BbvCI 2086 CCTCAGC CCTCAGC(-5/-2)
BciVI 463 GGATAC GTATCC(6/5)
BfrI 2643 CTTAAG C^TTAAG
BglI 2964 GCCGACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2009 AGATCT A^GATCT
BlnI 3499 CCTAGG C^CTAGG
BlpI 669 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 669 GCTCAGC GC^T[ACGT]AGC
BpuAI 2169 GAAGAC GAAGAC(2/6)
BsaHI 274 GGCGCC G[GA]^CG[CT]C
BsaI 3548 GAGACC GGTCTC(1/5)
BseAI 751 TCCGGA T^CCGGA
BsiHKAI 1302 GTGCTC G[AT]GC[AT]^C
BspEI 751 TCCGGA T^CCGGA
BssHII 2649 GCGCGC G^CGCGC
BstAPI 4389 GCACACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2315 GGTGACC G^GT[ACGT]ACC
BstPI 2315 GGTGACC G^GT[ACGT]ACC
CelII 669 GCTCAGC GC^T[ACGT]AGC
DraI 3763 TTTAAA TTT^AAA
DrdI 4076 GACCACTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2315 GGTGACC G^GT[ACGT]ACC
EcoRI 3952 GAATTC G^AATTC
HaeII 274 GGCGCC [GA]GCGC^[CT]
Hin1I 274 GGCGCC G[GA]^CG[CT]C
KasI 274 GGCGCC G^GCGCC
MfeI 2439 CAATTG C^AATTG
MroI 751 TCCGGA T^CCGGA
MunI 2439 CAATTG C^AATTG
NarI 274 GGCGCC GG^CGCC
PshBI 4311 ATTAAT AT^TAAT
SexAI 2251 ACCAGGT A^CC[AT]GGT
SfoI 274 GGCGCC GGC^GCC
SmaI 2974 CCCGGG CCC^GGG
SphI 1029 GCATGC GCATG^C
XbaI 647 TCTAGA T^CTAGA
XmaCI 2974 CCCGGG C^CCGGG
XmaI 2974 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaI 2974 CCCGGG C^[CT]CG[GA]G
3085 CCCGAG
BanI 192 GGTGCC G^G[CT][GA]CC
274 GGCGCC
BanII 285 GAGCCC G[GA]GC[CT]^C
1137 GGGCTC
BsaAI 1959 TACGTG [CT]AC^GT[GA]
3432 TACGTG
BsaWI 751 TCCGGA [AT]^CCGG[AT]
1363 ACCGGA
BsoBI 2974 CCCGGG C^[CT]CG[GA]G
3085 CCCGAG
BspHI 2306 TCATGA T^CATGA
4409 TCATGA
BspLU11I 4289 ACATGT A^CATGT
4323 ACATGT
BstXI 677 CCAGGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3129 CCAGTGCGCTGG
DsaI 196 CCATGG C^C[GA][CT]GG
2846 CCATGG
FauI 3247 GCGGG CCCGC(4/6)
3353 GCGGG
HincII 484 GTCAAC GT[CT]^[GA]AC
1977 GTCAAC
HindII 484 GTCAAC GT[CT]^[GA]AC
1977 GTCAAC
NcoI 196 CCATGG C^CATGG
2846 CCATGG
NdeI 3609 CATATG CA^TATG
3743 CATATG
PstI 1014 CTGCAG CTGCA^G
1397 CTGCAG
RcaI 2306 TCATGA T^CATGA
4409 TCATGA
XcmI 827 CCATGAAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2577 CCAGTGCTTGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI ApoI AvaII
BalI BamHI BbvI BclI BcnI
BfaI BmyI BpmI BsaBI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmBI BsmFI BsmI Bsp1286I Bsp1407I
BspMI BsrDI BsrGI BsrI BssKI
Bst1107I BstF5I BstNI BstUI BstYI
BstZ17I Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I EcoT22I FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII