##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI BbrPI
BciVI BclI BfrI BsaAI BsaBI
BsiWI BspDI BspHI BsrDI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
Eam1105I EarI Eco47III EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HpaI KpnI Ksp632I MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI SexAI SfuI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 839 TCCGGA T^CCGGA
AviII 1033 TGCGCA TGC^GCA
AvrII 2820 CCTAGG C^CTAGG
BalI 2979 TGGCCA TGG^CCA
BamHI 1210 GGATCC G^GATCC
BlnI 2820 CCTAGG C^CTAGG
BlpI 2770 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2770 GCTCAGC GC^T[ACGT]AGC
BseAI 839 TCCGGA T^CCGGA
BsiEI 328 CGGCCG CG[GA][CT]^CG
BsmBI 135 GAGACG CGTCTC(1/5)
BsmI 1970 GAATGC GAATGC(1/-1)
Bsp1407I 3016 TGTACA T^GTACA
BspEI 839 TCCGGA T^CCGGA
BsrGI 3016 TGTACA T^GTACA
BstEII 1928 GGTGACC G^GT[ACGT]ACC
BstPI 1928 GGTGACC G^GT[ACGT]ACC
CelII 2770 GCTCAGC GC^T[ACGT]AGC
DraIII 685 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 965 GACCTTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 328 CGGCCG C^GGCCG
EclXI 328 CGGCCG C^GGCCG
Eco52I 328 CGGCCG C^GGCCG
EcoO65I 1928 GGTGACC G^GT[ACGT]ACC
FspI 1033 TGCGCA TGC^GCA
HindIII 2463 AAGCTT A^AGCTT
MluNI 2979 TGGCCA TGG^CCA
MroI 839 TCCGGA T^CCGGA
MscI 2979 TGGCCA TGG^CCA
NaeI 2068 GCCGGC GCC^GGC
NgoMIV 2068 GCCGGC G^CCGGC
PstI 2013 CTGCAG CTGCA^G
ScaI 1072 AGTACT AGT^ACT
SfiI 263 GGCCACTTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2067 CGCCGGCG C[GA]^CCGG[CT]G
SphI 1900 GCATGC GCATG^C
SspBI 3016 TGTACA T^GTACA
Tsp509I 3011 AATT ^AATT
XcmI 874 CCACGTCCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 1141 ACATGT A^C[GA][CT]GT
1756 ACATGT
AocI 670 CCTCAGG CC^T[ACGT]AGG
769 CCTCAGG
BcgI 332 CGAGACCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1373 CGAGATGTTTGC
BglII 1540 AGATCT A^GATCT
1606 AGATCT
BsaWI 809 TCCGGT [AT]^CCGG[AT]
839 TCCGGA
BspLU11I 1141 ACATGT A^CATGT
1756 ACATGT
BssHII 11 GCGCGC G^CGCGC
13 GCGCGC
BssSI 1371 CACGAG CACGAG(-5/-1)
2058 CACGAG
BstAPI 684 GCACGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1895 GCAGTGCATGC
Bsu36I 670 CCTCAGG CC^T[ACGT]AGG
769 CCTCAGG
Eco81I 670 CCTCAGG CC^T[ACGT]AGG
769 CCTCAGG
EcoNI 777 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2199 CCTGCCCCAGG
MaeII 876 ACGT A^CGT
1108 ACGT
MseI 1777 TTAA T^TAA
3109 TTAA
PaeR7I 907 CTCGAG C^TCGAG
1387 CTCGAG
SfcI 1568 CTACAG C^T[GA][CT]AG
2013 CTGCAG
Tru9I 1777 TTAA T^TAA
3109 TTAA
XhoI 907 CTCGAG C^TCGAG
1387 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI KspI MaeI MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfoI SmaI
SmlI StuI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I Van91I
XhoII XmaCI XmaI