##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Aor51HI ApaI AscI Asp718I
AspI BamHI BanII BbeI BbrPI
BfrI BglI BlpI Bpu1102I BsaAI
BsaBI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BsrGI BssHII
BstBI CelII ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I EcoRV
EcoT22I FseI HaeII KasI KpnI
KspI MamI MluI MroI NarI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspBI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 269 CGCG CG^CG
AcyI 37 GACGCC G[GA]^CG[CT]C
AhdI 411 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 4593 ATTAAT AT^TAAT
AsnI 4593 ATTAAT AT^TAAT
AspEI 411 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3273 TGCGCA TGC^GCA
BanI 3997 GGCACC G^G[CT][GA]CC
BglII 4619 AGATCT A^GATCT
BsaHI 37 GACGCC G[GA]^CG[CT]C
BsaWI 979 ACCGGA [AT]^CCGG[AT]
BsrBI 276 CCGCTC CCGCTC(-3/-3)
BsrDI 2782 GCAATG GCAATG(2/0)
BsrFI 311 GCCGGC [GA]^CCGG[CT]
BssSI 2219 CTCGTG CACGAG(-5/-1)
BstEII 235 GGTCACC G^GT[ACGT]ACC
BstPI 235 GGTCACC G^GT[ACGT]ACC
BstUI 269 CGCG CG^CG
Cfr10I 311 GCCGGC [GA]^CCGG[CT]
DraIII 1095 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 411 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 235 GGTCACC G^GT[ACGT]ACC
EcoRI 4549 GAATTC G^AATTC
FspI 3273 TGCGCA TGC^GCA
Hin1I 37 GACGCC G[GA]^CG[CT]C
HindIII 4452 AAGCTT A^AGCTT
MfeI 1625 CAATTG C^AATTG
MunI 1625 CAATTG C^AATTG
MvnI 269 CGCG CG^CG
NaeI 311 GCCGGC GCC^GGC
NdeI 1290 CATATG CA^TATG
NgoMIV 311 GCCGGC G^CCGGC
PshAI 3513 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4593 ATTAAT AT^TAAT
SbfI 261 CCTGCAGG CCTGCA^GG
SexAI 1132 ACCAGGT A^CC[AT]GGT
SmaI 2819 CCCGGG CCC^GGG
Sse8387I 261 CCTGCAGG CCTGCA^GG
TaqI 572 TCGA T^CGA
TthHB8I 572 TCGA T^CGA
XbaI 1692 TCTAGA T^CTAGA
XcmI 913 CCAACTTGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2819 CCCGGG C^CCGGG
XmaI 2819 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Alw44I 2535 GTGCAC G^TGCAC
3085 GTGCAC
AlwNI 17 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
2168 CAGCCGCTG
ApaLI 2535 GTGCAC G^TGCAC
3085 GTGCAC
AvrII 728 CCTAGG C^CTAGG
1878 CCTAGG
BbsI 1969 GTCTTC GAAGAC(2/6)
2132 GTCTTC
BcgI 2831 CGATCCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3231 CGATTTTCCTGC
BciVI 818 GTATCC GTATCC(6/5)
2646 GTATCC
BclI 989 TGATCA T^GATCA
1750 TGATCA
BcnI 2819 CCCGG CC^[GC]GG
2820 CCGGG
BlnI 728 CCTAGG C^CTAGG
1878 CCTAGG
BpmI 4716 CTCCAG CTGGAG(16/14)
3151 CTGGAG
BpuAI 1969 GTCTTC GAAGAC(2/6)
2132 GTCTTC
BsaI 740 GAGACC GGTCTC(1/5)
3343 GGTCTC
BsmBI 73 GAGACG CGTCTC(1/5)
2747 GAGACG
BspLU11I 2394 ACATGT A^CATGT
3476 ACATGT
Bst1107I 2689 GTATAC GTA^TAC
3579 GTATAC
BstAPI 1094 GCACAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2167 GCAGCCGCTGC
BstZ17I 2689 GTATAC GTA^TAC
3579 GTATAC
CfoI 268 GCGC GCG^C
3274 GCGC
DraI 1005 TTTAAA TTT^AAA
3838 TTTAAA
DraII 409 AGGACCC [GA]G^G[ACGT]CC[CT]
1546 GGGACCT
DsaI 547 CCATGG C^C[GA][CT]GG
2651 CCATGG
EcoO109I 409 AGGACCC [GA]G^G[ACGT]CC[CT]
1546 GGGACCT
FbaI 989 TGATCA T^GATCA
1750 TGATCA
HgaI 37 GACGC GACGC(5/10)
270 GCGTC
HhaI 268 GCGC GCG^C
3274 GCGC
HinP1I 268 GCGC G^CGC
3274 GCGC
HpaI 1912 GTTAAC GTT^AAC
3532 GTTAAC
NciI 2819 CCCGG CC^[GC]GG
2820 CCGGG
NcoI 547 CCATGG C^CATGG
2651 CCATGG
PflMI 2969 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4001 CCATTAAATGG
PpuMI 409 AGGACCC [GA]G^G[AT]CC[CT]
1546 GGGACCT
SapI 209 GAAGAGC GCTCTTC(1/4)
810 GAAGAGC
StuI 258 AGGCCT AGG^CCT
3706 AGGCCT
Van91I 2969 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4001 CCATTAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AocI ApoI Asp700I
AspHI AvaI AvaII BalI BbvCI
BbvI BfaI BmyI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII EaeI EarI Eco81I EcoNI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII XmnI