##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AvrII BbeI BclI
BfrI BlnI BsiWI BspHI BspLU11I
Bst1107I BstEII BstPI BstZ17I CpoI
DrdI Eam1105I Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HpaI
KasI MfeI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SalI SapI SbfI
ScaI SfcI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 378 TCCGGA T^CCGGA
AcyI 2714 GACGCC G[GA]^CG[CT]C
AocI 714 CCTTAGG CC^T[ACGT]AGG
AviII 2513 TGCGCA TGC^GCA
BbrPI 3554 CACGTG CAC^GTG
BcgI 2363 GCAGTGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2879 AGATCT A^GATCT
BlpI 873 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 873 GCTCAGC GC^T[ACGT]AGC
BsaBI 3169 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2714 GACGCC G[GA]^CG[CT]C
BsaI 528 GAGACC GGTCTC(1/5)
BseAI 378 TCCGGA T^CCGGA
Bsp1407I 3620 TGTACA T^GTACA
BspDI 3634 ATCGAT AT^CGAT
BspEI 378 TCCGGA T^CCGGA
BspMI 494 ACCTGC ACCTGC(4/8)
BsrDI 2892 CATTGC GCAATG(2/0)
BsrGI 3620 TGTACA T^GTACA
BssHII 2765 GCGCGC G^CGCGC
BssSI 158 CTCGTG CACGAG(-5/-1)
BstAPI 489 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 714 CCTTAGG CC^T[ACGT]AGG
CelII 873 GCTCAGC GC^T[ACGT]AGC
ClaI 3634 ATCGAT AT^CGAT
DraIII 806 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 714 CCTTAGG CC^T[ACGT]AGG
FspI 2513 TGCGCA TGC^GCA
Hin1I 2714 GACGCC G[GA]^CG[CT]C
MamI 3169 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 3324 ACGCGT A^CGCGT
MroI 378 TCCGGA T^CCGGA
NspI 3289 GCATGT [GA]CATG^[CT]
PflMI 406 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3554 CACGTG CAC^GTG
PmlI 3554 CACGTG CAC^GTG
SexAI 843 ACCTGGT A^CC[AT]GGT
SspBI 3620 TGTACA T^GTACA
SwaI 3245 ATTTAAAT ATTT^AAAT
Van91I 406 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1426 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 614 GGTACC G^GTACC
3668 GGTACC
AflIII 3324 ACGCGT A^C[GA][CT]GT
3717 ACGTGT
AlwNI 407 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
490 CAGCACCTG
Asp718I 614 GGTACC G^GTACC
3668 GGTACC
BbsI 828 GAAGAC GAAGAC(2/6)
3328 GTCTTC
BciVI 3320 GGATAC GTATCC(6/5)
3698 GTATCC
BglI 1742 GCCAGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2302 GCCAGACAGGC
BpuAI 828 GAAGAC GAAGAC(2/6)
3328 GTCTTC
BsaAI 3554 CACGTG [CT]AC^GT[GA]
3716 TACGTG
BsaWI 202 ACCGGA [AT]^CCGG[AT]
378 TCCGGA
BsgI 2923 CTGCAC GTGCAG(16/14)
848 GTGCAG
BsmBI 2818 CGTCTC CGTCTC(1/5)
2215 GAGACG
BsrFI 731 GCCGGC [GA]^CCGG[CT]
800 GCCGGC
BstBI 354 TTCGAA TT^CGAA
444 TTCGAA
Cfr10I 731 GCCGGC [GA]^CCGG[CT]
800 GCCGGC
EcoNI 702 CCTCACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1144 CCTGGAGAAGG
HincII 68 GTCAAC GT[CT]^[GA]AC
3228 GTTGAC
HindII 68 GTCAAC GT[CT]^[GA]AC
3228 GTTGAC
HindIII 1875 AAGCTT A^AGCTT
3661 AAGCTT
KpnI 614 GGTACC GGTAC^C
3668 GGTACC
KspI 2457 CCGCGG CCGC^GG
2676 CCGCGG
MslI 1162 CACGGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3108 CATGATTGTG
NaeI 731 GCCGGC GCC^GGC
800 GCCGGC
NgoMIV 731 GCCGGC G^CCGGC
800 GCCGGC
NspV 354 TTCGAA TT^CGAA
444 TTCGAA
PvuII 818 CAGCTG CAG^CTG
876 CAGCTG
SacII 2457 CCGCGG CCGC^GG
2676 CCGCGG
SfiI 1741 GGCCAGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2301 GGCCAGACAGGCC
SfuI 354 TTCGAA TT^CGAA
444 TTCGAA
SmlI 749 CTCAAG C^T[CT][GA]AG
3651 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
ApaI AspHI AvaI AvaII BalI
BamHI BanI BanII BbvCI BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SmaI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI