################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII AccI AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AvrII BbeI BclI BfrI BlnI BsiWI BspHI BspLU11I Bst1107I BstEII BstPI BstZ17I CpoI DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HpaI KasI MfeI MunI NarI NcoI NdeI NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SapI SbfI ScaI SfcI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 378 TCCGGA T^CCGGA AcyI 2714 GACGCC G[GA]^CG[CT]C AocI 714 CCTTAGG CC^T[ACGT]AGG AviII 2513 TGCGCA TGC^GCA BbrPI 3554 CACGTG CAC^GTG BcgI 2363 GCAGTGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 2879 AGATCT A^GATCT BlpI 873 GCTCAGC GC^T[ACGT]AGC Bpu1102I 873 GCTCAGC GC^T[ACGT]AGC BsaBI 3169 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2714 GACGCC G[GA]^CG[CT]C BsaI 528 GAGACC GGTCTC(1/5) BseAI 378 TCCGGA T^CCGGA Bsp1407I 3620 TGTACA T^GTACA BspDI 3634 ATCGAT AT^CGAT BspEI 378 TCCGGA T^CCGGA BspMI 494 ACCTGC ACCTGC(4/8) BsrDI 2892 CATTGC GCAATG(2/0) BsrGI 3620 TGTACA T^GTACA BssHII 2765 GCGCGC G^CGCGC BssSI 158 CTCGTG CACGAG(-5/-1) BstAPI 489 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 714 CCTTAGG CC^T[ACGT]AGG CelII 873 GCTCAGC GC^T[ACGT]AGC ClaI 3634 ATCGAT AT^CGAT DraIII 806 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 714 CCTTAGG CC^T[ACGT]AGG FspI 2513 TGCGCA TGC^GCA Hin1I 2714 GACGCC G[GA]^CG[CT]C MamI 3169 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 3324 ACGCGT A^CGCGT MroI 378 TCCGGA T^CCGGA NspI 3289 GCATGT [GA]CATG^[CT] PflMI 406 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3554 CACGTG CAC^GTG PmlI 3554 CACGTG CAC^GTG SexAI 843 ACCTGGT A^CC[AT]GGT SspBI 3620 TGTACA T^GTACA SwaI 3245 ATTTAAAT ATTT^AAAT Van91I 406 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1426 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### Acc65I 614 GGTACC G^GTACC 3668 GGTACC AflIII 3324 ACGCGT A^C[GA][CT]GT 3717 ACGTGT AlwNI 407 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 490 CAGCACCTG Asp718I 614 GGTACC G^GTACC 3668 GGTACC BbsI 828 GAAGAC GAAGAC(2/6) 3328 GTCTTC BciVI 3320 GGATAC GTATCC(6/5) 3698 GTATCC BglI 1742 GCCAGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2302 GCCAGACAGGC BpuAI 828 GAAGAC GAAGAC(2/6) 3328 GTCTTC BsaAI 3554 CACGTG [CT]AC^GT[GA] 3716 TACGTG BsaWI 202 ACCGGA [AT]^CCGG[AT] 378 TCCGGA BsgI 2923 CTGCAC GTGCAG(16/14) 848 GTGCAG BsmBI 2818 CGTCTC CGTCTC(1/5) 2215 GAGACG BsrFI 731 GCCGGC [GA]^CCGG[CT] 800 GCCGGC BstBI 354 TTCGAA TT^CGAA 444 TTCGAA Cfr10I 731 GCCGGC [GA]^CCGG[CT] 800 GCCGGC EcoNI 702 CCTCACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1144 CCTGGAGAAGG HincII 68 GTCAAC GT[CT]^[GA]AC 3228 GTTGAC HindII 68 GTCAAC GT[CT]^[GA]AC 3228 GTTGAC HindIII 1875 AAGCTT A^AGCTT 3661 AAGCTT KpnI 614 GGTACC GGTAC^C 3668 GGTACC KspI 2457 CCGCGG CCGC^GG 2676 CCGCGG MslI 1162 CACGGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3108 CATGATTGTG NaeI 731 GCCGGC GCC^GGC 800 GCCGGC NgoMIV 731 GCCGGC G^CCGGC 800 GCCGGC NspV 354 TTCGAA TT^CGAA 444 TTCGAA PvuII 818 CAGCTG CAG^CTG 876 CAGCTG SacII 2457 CCGCGG CCGC^GG 2676 CCGCGG SfiI 1741 GGCCAGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 2301 GGCCAGACAGGCC SfuI 354 TTCGAA TT^CGAA 444 TTCGAA SmlI 749 CTCAAG C^T[CT][GA]AG 3651 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccII AciI AfaI AluI AlwI ApaI AspHI AvaI AvaII BalI BamHI BanI BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SmaI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI