##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Aor51HI ApaI Asp718I AspEI
AviII AvrII BalI BamHI BbvCI
BcgI BlnI BlpI BpmI Bpu1102I
BsaBI BsaI BseAI BsgI BsmBI
BsmI BspEI BsrBI BsrFI BssSI
BstAPI BstEII BstPI CelII Cfr10I
CpoI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HgaI
HpaI KpnI MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NcoI NgoMIV NheI NotI NsiI
PacI PaeR7I PinAI PmeI PpuMI
PshAI Psp1406I PvuI RsrII SbfI
SexAI SfiI SgrAI SmaI SpeI
SphI Sse8387I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AcyI 168 GGCGCC G[GA]^CG[CT]C
Alw44I 3923 GTGCAC G^TGCAC
AlwNI 3417 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 299 CCTTAGG CC^T[ACGT]AGG
ApaLI 3923 GTGCAC G^TGCAC
AscI 62 GGCGCGCC GG^CGCGCC
Asp700I 3867 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2847 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 168 GGCGCC GGCGC^C
BbrPI 2018 CACGTG CAC^GTG
BglI 3344 GCCGAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1584 AGATCT A^GATCT
BsaHI 168 GGCGCC G[GA]^CG[CT]C
BsaWI 2829 TCCGGT [AT]^CCGG[AT]
BsiEI 2813 CGGTCG CG[GA][CT]^CG
BsiWI 2704 CGTACG C^GTACG
BspDI 1848 ATCGAT AT^CGAT
BspHI 2843 TCATGA T^CATGA
BsrDI 2185 GCAATG GCAATG(2/0)
BssHII 63 GCGCGC G^CGCGC
BstBI 826 TTCGAA TT^CGAA
BstXI 746 CCACTACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 299 CCTTAGG CC^T[ACGT]AGG
ClaI 1848 ATCGAT AT^CGAT
DraII 295 GGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 1811 GACTTTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 68 CCGCGG C^C[GA][CT]GG
Eco81I 299 CCTTAGG CC^T[ACGT]AGG
EcoO109I 295 GGGGCCT [GA]G^G[ACGT]CC[CT]
HaeII 168 GGCGCC [GA]GCGC^[CT]
Hin1I 168 GGCGCC G[GA]^CG[CT]C
KasI 168 GGCGCC G^GCGCC
KspI 68 CCGCGG CCGC^GG
NarI 168 GGCGCC GG^CGCC
NdeI 2930 CATATG CA^TATG
NruI 48 TCGCGA TCG^CGA
NspV 826 TTCGAA TT^CGAA
PflFI 2847 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2413 CCACCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2018 CACGTG CAC^GTG
PmlI 2018 CACGTG CAC^GTG
RcaI 2843 TCATGA T^CATGA
SacI 2342 GAGCTC GAGCT^C
SacII 68 CCGCGG CCGC^GG
SalI 2815 GTCGAC G^TCGAC
SapI 180 GAAGAGC GCTCTTC(1/4)
ScaI 2957 AGTACT AGT^ACT
SfoI 168 GGCGCC GGC^GCC
SfuI 826 TTCGAA TT^CGAA
SnaBI 4185 TACGTA TAC^GTA
StuI 1403 AGGCCT AGG^CCT
SwaI 1207 ATTTAAAT ATTT^AAAT
Tth111I 2847 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2413 CCACCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3563 TCTAGA T^CTAGA
XmnI 3867 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 3011 CTTAAG C^TTAAG
4093 CTTAAG
AspHI 2342 GAGCTC G[AT]GC[AT]^C
3923 GTGCAC
BanII 183 GAGCCC G[GA]GC[CT]^C
2342 GAGCTC
BciVI 3078 GGATAC GTATCC(6/5)
2391 GTATCC
BclI 1805 TGATCA T^GATCA
3895 TGATCA
BfrI 3011 CTTAAG C^TTAAG
4093 CTTAAG
BsiHKAI 2342 GAGCTC G[AT]GC[AT]^C
3923 GTGCAC
Bsp1407I 3504 TGTACA T^GTACA
4203 TGTACA
BspMI 3379 ACCTGC ACCTGC(4/8)
834 GCAGGT
BsrGI 3504 TGTACA T^GTACA
4203 TGTACA
Bst1107I 1373 GTATAC GTA^TAC
2449 GTATAC
BstNI 429 CCTGG CC^[AT]GG
622 CCAGG
BstYI 1584 AGATCT [GA]^GATC[CT]
3099 AGATCC
BstZ17I 1373 GTATAC GTA^TAC
2449 GTATAC
DraIII 1838 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1896 CACTGTGTG
EarI 180 GAAGAG CTCTTC(1/4)
1103 GAAGAG
EcoRII 429 CCTGG ^CC[AT]GG
622 CCAGG
EcoT14I 175 CCAAGG C^C[AT][AT]GG
2038 CCAAGG
FbaI 1805 TGATCA T^GATCA
3895 TGATCA
HincII 2693 GTCAAC GT[CT]^[GA]AC
2815 GTCGAC
HindII 2693 GTCAAC GT[CT]^[GA]AC
2815 GTCGAC
Ksp632I 180 GAAGAG CTCTTC(1/4)
1103 GAAGAG
MflI 1584 AGATCT [GA]^GATC[CT]
3099 AGATCC
MvaI 429 CCTGG CC^[AT]GG
622 CCAGG
SfaNI 137 GATGC GCATC(5/9)
287 GATGC
SspBI 3504 TGTACA T^GTACA
4203 TGTACA
StyI 175 CCAAGG C^C[AT][AT]GG
2038 CCAAGG
Tsp45I 1894 GTCAC ^GT[GC]AC
4130 GTCAC
XhoII 1584 AGATCT [GA]^GATC[CT]
3099 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI ApoI AseI
AsnI AvaI AvaII BanI BbsI
BbvI BcnI BfaI BmyI BpuAI
BsaAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspLU11I
BsrI BssKI BstF5I BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraI
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfcI SmlI SspI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I