##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbsI BcgI BglI BlnI BlpI
Bpu1102I BpuAI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BspDI BspEI
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I CelII
Cfr10I ClaI CpoI DraIII EaeI
EagI Eam1105I EclXI Eco47III Eco52I
FauI FseI FspI HpaI KpnI
KspI MfeI MluI MluNI MroI
MscI MslI MunI MvnI NaeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacI SacII SapI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I StuI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 478 GTCGAC GT^[AC][GT]AC
AciI 3923 GCGG CCGC(-3/-1)
AspHI 2884 GAGCAC G[AT]GC[AT]^C
AvaI 70 CCCGGG C^[CT]CG[GA]G
BanI 598 GGCGCC G^G[CT][GA]CC
BanII 529 GAGCCC G[GA]GC[CT]^C
BbeI 598 GGCGCC GGCGC^C
BbvCI 798 GCTGAGG CCTCAGC(-5/-2)
BciVI 4077 GTATCC GTATCC(6/5)
BglII 1080 AGATCT A^GATCT
BsaAI 2961 CACGTA [CT]AC^GT[GA]
BsaBI 455 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2852 GAGACC GGTCTC(1/5)
BsiHKAI 2884 GAGCAC G[AT]GC[AT]^C
BsoBI 70 CCCGGG C^[CT]CG[GA]G
BstAPI 1305 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1043 GGTGACC G^GT[ACGT]ACC
BstPI 1043 GGTGACC G^GT[ACGT]ACC
BstXI 517 CCAGTAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 599 GCGC GCG^C
DrdI 340 GACAGGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1637 CCATGG C^C[GA][CT]GG
EcoO65I 1043 GGTGACC G^GT[ACGT]ACC
EcoRI 3178 GAATTC G^AATTC
EcoRV 1188 GATATC GAT^ATC
HaeII 598 GGCGCC [GA]GCGC^[CT]
HgaI 1229 GCGTC GACGC(5/10)
HhaI 599 GCGC GCG^C
HinP1I 599 GCGC G^CGC
HindIII 393 AAGCTT A^AGCTT
KasI 598 GGCGCC G^GCGCC
MamI 455 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 1515 CAGCTG C[AC]G^C[GT]G
NarI 598 GGCGCC GG^CGCC
NcoI 1637 CCATGG C^CATGG
NdeI 2414 CATATG CA^TATG
PshAI 1859 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1515 CAGCTG CAG^CTG
SalI 478 GTCGAC G^TCGAC
SfoI 598 GGCGCC GGC^GCC
SmaI 70 CCCGGG CCC^GGG
SphI 1540 GCATGC GCATG^C
XcmI 841 CCACTGGGATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 70 CCCGGG C^CCGGG
XmaI 70 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 598 GGCGCC G[GA]^CG[CT]C
1228 GGCGTC
AflII 2941 CTTAAG C^TTAAG
3281 CTTAAG
AflIII 590 ACATGT A^C[GA][CT]GT
2606 ACATGT
BfrI 2941 CTTAAG C^TTAAG
3281 CTTAAG
BsaHI 598 GGCGCC G[GA]^CG[CT]C
1228 GGCGTC
BsmI 1615 GAATGC GAATGC(1/-1)
1140 GCATTC
BspHI 2553 TCATGA T^CATGA
2880 TCATGA
BspLU11I 590 ACATGT A^CATGT
2606 ACATGT
BsrDI 1799 CATTGC GCAATG(2/0)
4038 CATTGC
BstYI 1080 AGATCT [GA]^GATC[CT]
3819 AGATCC
EcoNI 20 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
161 CCTGGCCCAGG
EcoT22I 1071 ATGCAT ATGCA^T
1720 ATGCAT
Hin1I 598 GGCGCC G[GA]^CG[CT]C
1228 GGCGTC
HincII 478 GTCGAC GT[CT]^[GA]AC
1734 GTCAAC
HindII 478 GTCGAC GT[CT]^[GA]AC
1734 GTCAAC
MaeII 2190 ACGT A^CGT
2962 ACGT
MflI 1080 AGATCT [GA]^GATC[CT]
3819 AGATCC
NsiI 1071 ATGCAT ATGCA^T
1720 ATGCAT
PflMI 1637 CCATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3305 CCATTAAGTGG
RcaI 2553 TCATGA T^CATGA
2880 TCATGA
TaqI 479 TCGA T^CGA
1976 TCGA
TthHB8I 479 TCGA T^CGA
1976 TCGA
Van91I 1637 CCATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3305 CCATTAAGTGG
XhoII 1080 AGATCT [GA]^GATC[CT]
3819 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
AocI ApoI AseI AsnI Asp700I
AvaII BbvI BclI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraI DraII EarI Eco81I EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PshBI PstI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XbaI XmnI