##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI AocI Aor51HI AscI Asp718I
AspEI AspI AviII BbeI BbrPI
BbvCI BcgI BcnI BglI BlpI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstPI
Bsu36I CelII Cfr10I ClaI CpoI
DraII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI FauI FseI FspI HaeII
Hin1I KasI KpnI MfeI MluI
MroI MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PvuI RcaI
RsrII SacI SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SwaI Tth111I XbaI XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccII 24 CGCG CG^CG
AclI 1363 AACGTT AA^CGTT
ApaI 1986 GGGCCC GGGCC^C
AvaII 1174 GGACC G^G[AT]CC
AvrII 3772 CCTAGG C^CTAGG
BamHI 1623 GGATCC G^GATCC
BanI 963 GGCACC G^G[CT][GA]CC
BanII 1986 GGGCCC G[GA]GC[CT]^C
BglII 3965 AGATCT A^GATCT
BlnI 3772 CCTAGG C^CTAGG
BsaI 3059 GGTCTC GGTCTC(1/5)
BsgI 294 GTGCAG GTGCAG(16/14)
BsmFI 2275 GTCCC GGGAC(10/14)
BspMI 3056 GCAGGT ACCTGC(4/8)
Bst1107I 1959 GTATAC GTA^TAC
BstUI 24 CGCG CG^CG
BstXI 1495 CCAAAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1959 GTATAC GTA^TAC
CfoI 1015 GCGC GCG^C
EcoNI 2089 CCTTTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2100 GATATC GAT^ATC
HapII 4 CCGG C^CGG
HgaI 16 GACGC GACGC(5/10)
HhaI 1015 GCGC GCG^C
HinP1I 1015 GCGC G^CGC
HindIII 426 AAGCTT A^AGCTT
HpaII 4 CCGG C^CGG
KspI 23 CCGCGG CCGC^GG
MspI 4 CCGG C^CGG
MvnI 24 CGCG CG^CG
NcoI 1276 CCATGG C^CATGG
PaeR7I 984 CTCGAG C^TCGAG
PflMI 3411 CCATTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 485 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1363 AACGTT AA^CGTT
SacII 23 CCGCGG CCGC^GG
StuI 1307 AGGCCT AGG^CCT
Van91I 3411 CCATTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 984 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflII 1968 CTTAAG C^TTAAG
3289 CTTAAG
Alw44I 418 GTGCAC G^TGCAC
2214 GTGCAC
ApaLI 418 GTGCAC G^TGCAC
2214 GTGCAC
AseI 179 ATTAAT AT^TAAT
2530 ATTAAT
AsnI 179 ATTAAT AT^TAAT
2530 ATTAAT
AvaI 984 CTCGAG C^[CT]CG[GA]G
1989 CCCGAG
BalI 2036 TGGCCA TGG^CCA
3408 TGGCCA
BciVI 717 GTATCC GTATCC(6/5)
1568 GTATCC
BclI 60 TGATCA T^GATCA
828 TGATCA
BfrI 1968 CTTAAG C^TTAAG
3289 CTTAAG
BsaBI 55 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2559 GATAAAAATC
BseRI 2837 CTCCTC GAGGAG(10/8)
3532 GAGGAG
BsmI 3052 GAATGC GAATGC(1/-1)
820 GCATTC
BsoBI 984 CTCGAG C^[CT]CG[GA]G
1989 CCCGAG
BstAPI 13 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1630 GCAGTATTTGC
Cac8I 221 GCTTGC GC[ACGT]^[ACGT]GC
794 GCAAGC
DsaI 23 CCGCGG C^C[GA][CT]GG
1276 CCATGG
EcoT14I 1276 CCATGG C^C[AT][AT]GG
3772 CCTAGG
EcoT22I 1028 ATGCAT ATGCA^T
2808 ATGCAT
FbaI 60 TGATCA T^GATCA
828 TGATCA
HincII 2845 GTTAAC GT[CT]^[GA]AC
2980 GTTAAC
HindII 2845 GTTAAC GT[CT]^[GA]AC
2980 GTTAAC
HpaI 2845 GTTAAC GTT^AAC
2980 GTTAAC
MaeII 1364 ACGT A^CGT
2377 ACGT
MamI 55 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2559 GATAAAAATC
MluNI 2036 TGGCCA TGG^CCA
3408 TGGCCA
MscI 2036 TGGCCA TGG^CCA
3408 TGGCCA
NsiI 1028 ATGCAT ATGCA^T
2808 ATGCAT
PshBI 179 ATTAAT AT^TAAT
2530 ATTAAT
PstI 2225 CTGCAG CTGCA^G
2746 CTGCAG
PvuII 744 CAGCTG CAG^CTG
1812 CAGCTG
SpeI 3100 ACTAGT A^CTAGT
3453 ACTAGT
StyI 1276 CCATGG C^C[AT][AT]GG
3772 CCTAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BspLU11I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII DraI DraIII
EaeI EarI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI