##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BcgI BcnI BfrI BglI
BlnI BpmI BsaBI BsaHI BsaI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CfoI
ClaI CpoI DraII DraIII EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I KasI KpnI KspI MamI
MluI MroI MvnI NaeI NarI
NciI NgoMIV NotI NruI NspV
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PpuMI PshAI PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SmlI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AciI 1440 GCGG CCGC(-3/-1)
AclI 349 AACGTT AA^CGTT
AgeI 1552 ACCGGT A^CCGGT
AhdI 756 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 94 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 756 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1812 GAGCTC G[AT]GC[AT]^C
AvaII 3559 GGACC G^G[AT]CC
BalI 38 TGGCCA TGG^CCA
BanII 1812 GAGCTC G[GA]GC[CT]^C
BbvCI 728 GCTGAGG CCTCAGC(-5/-2)
BlpI 2893 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2893 GCTCAGC GC^T[ACGT]AGC
BsaAI 3913 TACGTA [CT]AC^GT[GA]
BsaWI 1552 ACCGGT [AT]^CCGG[AT]
BsgI 456 CTGCAC GTGCAG(16/14)
BsiHKAI 1812 GAGCTC G[AT]GC[AT]^C
BspMI 2321 GCAGGT ACCTGC(4/8)
BsrFI 1552 ACCGGT [GA]^CCGG[CT]
CelII 2893 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1552 ACCGGT [GA]^CCGG[CT]
DrdI 3625 GACCTGTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 38 TGGCCA [CT]^GGCC[GA]
Eam1105I 756 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3847 GAATTC G^AATTC
HapII 1553 CCGG C^CGG
HindIII 237 AAGCTT A^AGCTT
HpaI 2707 GTTAAC GTT^AAC
HpaII 1553 CCGG C^CGG
MluNI 38 TGGCCA TGG^CCA
MscI 38 TGGCCA TGG^CCA
MspA1I 173 CAGCTG C[AC]G^C[GT]G
MspI 1553 CCGG C^CGG
PinAI 1552 ACCGGT A^CCGGT
Psp1406I 349 AACGTT AA^CGTT
PstI 3931 CTGCAG CTGCA^G
PvuII 173 CAGCTG CAG^CTG
SacI 1812 GAGCTC GAGCT^C
SapI 1809 GAAGAGC GCTCTTC(1/4)
ScaI 3686 AGTACT AGT^ACT
SnaBI 3913 TACGTA TAC^GTA
TaqI 1748 TCGA T^CGA
TthHB8I 1748 TCGA T^CGA
XcmI 2571 CCACATGTGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BglII 2774 AGATCT A^GATCT
3209 AGATCT
BmyI 1812 GAGCTC G[AGT]GC[ACT]^C
2876 GTGCCC
BsmFI 3558 GGGAC GGGAC(10/14)
764 GTCCC
Bsp1286I 1812 GAGCTC G[AGT]GC[ACT]^C
2876 GTGCCC
BspHI 402 TCATGA T^CATGA
2674 TCATGA
BsrDI 1817 CATTGC GCAATG(2/0)
2579 GCAATG
BstXI 1001 CCAGGTAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1964 CCAAGAAAATGG
Cfr13I 3101 GGGCC G^G[ACGT]CC
3559 GGACC
DsaI 825 CCATGG C^C[GA][CT]GG
3602 CCATGG
EarI 738 GAAGAG CTCTTC(1/4)
1809 GAAGAG
EcoT22I 476 ATGCAT ATGCA^T
1091 ATGCAT
Ksp632I 738 GAAGAG CTCTTC(1/4)
1809 GAAGAG
MfeI 1675 CAATTG C^AATTG
2240 CAATTG
MunI 1675 CAATTG C^AATTG
2240 CAATTG
NcoI 825 CCATGG C^CATGG
3602 CCATGG
NheI 47 GCTAGC G^CTAGC
2293 GCTAGC
NsiI 476 ATGCAT ATGCA^T
1091 ATGCAT
RcaI 402 TCATGA T^CATGA
2674 TCATGA
Sau96I 3101 GGGCC G^G[ACGT]CC
3559 GGACC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI BanI
BbsI BbvI BciVI BclI BfaI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI Bsp1407I BspLU11I BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
DdeI DpnII DraI EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeI NdeII NlaIII NlaIV NspI
PacI PleI PshBI RsaI Sau3AI
ScrFI SexAI SfaNI SfcI SspBI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII