##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
Acc65I AccIII AflII AgeI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AviII BalI BamHI BbrPI BfrI
BlpI Bpu1102I BseAI BsiWI BspEI
Bst1107I BstAPI BstEII BstPI BstZ17I
CelII CpoI DraIII DrdI Eco47III
EcoO65I FseI FspI KpnI MluI
MluNI MroI MscI NcoI NheI
NotI NruI PacI PinAI PmaCI
PmeI PmlI RsrII SbfI SfiI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 5712 GACGTC GACGT^C
AclI 1491 AACGTT AA^CGTT
AhdI 4971 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2839 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4971 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2739 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 2988 CGAAACAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 356 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 995 CACGTA [CT]AC^GT[GA]
BsaI 2468 GAGACC GGTCTC(1/5)
BsaWI 66 ACCGGA [AT]^CCGG[AT]
BsmBI 1750 GAGACG CGTCTC(1/5)
Bsp1407I 511 TGTACA T^GTACA
BspDI 3367 ATCGAT AT^CGAT
BsrBI 186 GAGCGG CCGCTC(-3/-3)
BsrDI 1334 GCAATG GCAATG(2/0)
BsrGI 511 TGTACA T^GTACA
BssSI 908 CTCGTG CACGAG(-5/-1)
ClaI 3367 ATCGAT AT^CGAT
DsaI 351 CCGCGG C^C[GA][CT]GG
Eam1105I 4971 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4331 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4621 GATATC GAT^ATC
HindIII 3805 AAGCTT A^AGCTT
KspI 351 CCGCGG CCGC^GG
PaeR7I 764 CTCGAG C^TCGAG
PflFI 2739 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 884 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2894 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1491 AACGTT AA^CGTT
PvuI 400 CGATCG CGAT^CG
SacII 351 CCGCGG CCGC^GG
SalI 3581 GTCGAC G^TCGAC
SapI 2875 GCTCTTC GCTCTTC(1/4)
SexAI 2471 ACCAGGT A^CC[AT]GGT
SpeI 3904 ACTAGT A^CTAGT
SspBI 511 TGTACA T^GTACA
StuI 3050 AGGCCT AGG^CCT
Tth111I 2739 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 764 CTCGAG C^TCGAG
XmnI 2839 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AocI 99 CCTGAGG CC^T[ACGT]AGG
535 CCTCAGG
AseI 3466 ATTAAT AT^TAAT
6456 ATTAAT
AsnI 3466 ATTAAT AT^TAAT
6456 ATTAAT
AvaII 885 GGTCC G^G[AT]CC
1769 GGTCC
AvrII 546 CCTAGG C^CTAGG
4854 CCTAGG
BbvCI 243 CCTCAGC CCTCAGC(-5/-2)
368 CCTCAGC
BclI 1794 TGATCA T^GATCA
2205 TGATCA
BlnI 546 CCTAGG C^CTAGG
4854 CCTAGG
BsaBI 5608 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5963 GATCTTAATC
BsgI 3474 CTGCAC GTGCAG(16/14)
2975 GTGCAG
BssHII 135 GCGCGC G^CGCGC
152 GCGCGC
BstBI 1928 TTCGAA TT^CGAA
4646 TTCGAA
BstXI 416 CCAGCATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3854 CCACTCAGATGG
Bsu36I 99 CCTGAGG CC^T[ACGT]AGG
535 CCTCAGG
DraII 884 AGGTCCT [GA]G^G[ACGT]CC[CT]
4857 AGGCCCT
EagI 92 CGGCCG C^GGCCG
308 CGGCCG
EclXI 92 CGGCCG C^GGCCG
308 CGGCCG
Eco52I 92 CGGCCG C^GGCCG
308 CGGCCG
Eco81I 99 CCTGAGG CC^T[ACGT]AGG
535 CCTCAGG
EcoO109I 884 AGGTCCT [GA]G^G[ACGT]CC[CT]
4857 AGGCCCT
EcoT22I 1760 ATGCAT ATGCA^T
2996 ATGCAT
FbaI 1794 TGATCA T^GATCA
2205 TGATCA
HgaI 324 GACGC GACGC(5/10)
58 GCGTC
HpaI 730 GTTAAC GTT^AAC
4842 GTTAAC
MamI 5608 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5963 GATCTTAATC
MfeI 2644 CAATTG C^AATTG
5621 CAATTG
MunI 2644 CAATTG C^AATTG
5621 CAATTG
NsiI 1760 ATGCAT ATGCA^T
2996 ATGCAT
NspV 1928 TTCGAA TT^CGAA
4646 TTCGAA
PflMI 170 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2835 CCAAGAAGTGG
PshBI 3466 ATTAAT AT^TAAT
6456 ATTAAT
PvuII 2978 CAGCTG CAG^CTG
5306 CAGCTG
ScaI 3570 AGTACT AGT^ACT
3736 AGTACT
SfuI 1928 TTCGAA TT^CGAA
4646 TTCGAA
Van91I 170 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2835 CCAAGAAGTGG
XcmI 3197 CCATTTCCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3851 CCACCACTCAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI AspHI AvaI BanI BanII
BbeI BbsI BbvI BciVI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspLU11I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI EaeI
EarI EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PstI RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII