################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## Acc65I AccIII AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AviII BalI BamHI BbrPI BfrI BlpI Bpu1102I BseAI BsiWI BspEI Bst1107I BstAPI BstEII BstPI BstZ17I CelII CpoI DraIII DrdI Eco47III EcoO65I FseI FspI KpnI MluI MluNI MroI MscI NcoI NheI NotI NruI PacI PinAI PmaCI PmeI PmlI RsrII SbfI SfiI SgrAI SmaI SnaBI SphI Sse8387I SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 5712 GACGTC GACGT^C AclI 1491 AACGTT AA^CGTT AhdI 4971 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 2839 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 4971 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2739 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BcgI 2988 CGAAACAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 356 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 995 CACGTA [CT]AC^GT[GA] BsaI 2468 GAGACC GGTCTC(1/5) BsaWI 66 ACCGGA [AT]^CCGG[AT] BsmBI 1750 GAGACG CGTCTC(1/5) Bsp1407I 511 TGTACA T^GTACA BspDI 3367 ATCGAT AT^CGAT BsrBI 186 GAGCGG CCGCTC(-3/-3) BsrDI 1334 GCAATG GCAATG(2/0) BsrGI 511 TGTACA T^GTACA BssSI 908 CTCGTG CACGAG(-5/-1) ClaI 3367 ATCGAT AT^CGAT DsaI 351 CCGCGG C^C[GA][CT]GG Eam1105I 4971 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 4331 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 4621 GATATC GAT^ATC HindIII 3805 AAGCTT A^AGCTT KspI 351 CCGCGG CCGC^GG PaeR7I 764 CTCGAG C^TCGAG PflFI 2739 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 884 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 2894 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1491 AACGTT AA^CGTT PvuI 400 CGATCG CGAT^CG SacII 351 CCGCGG CCGC^GG SalI 3581 GTCGAC G^TCGAC SapI 2875 GCTCTTC GCTCTTC(1/4) SexAI 2471 ACCAGGT A^CC[AT]GGT SpeI 3904 ACTAGT A^CTAGT SspBI 511 TGTACA T^GTACA StuI 3050 AGGCCT AGG^CCT Tth111I 2739 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 764 CTCGAG C^TCGAG XmnI 2839 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AocI 99 CCTGAGG CC^T[ACGT]AGG 535 CCTCAGG AseI 3466 ATTAAT AT^TAAT 6456 ATTAAT AsnI 3466 ATTAAT AT^TAAT 6456 ATTAAT AvaII 885 GGTCC G^G[AT]CC 1769 GGTCC AvrII 546 CCTAGG C^CTAGG 4854 CCTAGG BbvCI 243 CCTCAGC CCTCAGC(-5/-2) 368 CCTCAGC BclI 1794 TGATCA T^GATCA 2205 TGATCA BlnI 546 CCTAGG C^CTAGG 4854 CCTAGG BsaBI 5608 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5963 GATCTTAATC BsgI 3474 CTGCAC GTGCAG(16/14) 2975 GTGCAG BssHII 135 GCGCGC G^CGCGC 152 GCGCGC BstBI 1928 TTCGAA TT^CGAA 4646 TTCGAA BstXI 416 CCAGCATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3854 CCACTCAGATGG Bsu36I 99 CCTGAGG CC^T[ACGT]AGG 535 CCTCAGG DraII 884 AGGTCCT [GA]G^G[ACGT]CC[CT] 4857 AGGCCCT EagI 92 CGGCCG C^GGCCG 308 CGGCCG EclXI 92 CGGCCG C^GGCCG 308 CGGCCG Eco52I 92 CGGCCG C^GGCCG 308 CGGCCG Eco81I 99 CCTGAGG CC^T[ACGT]AGG 535 CCTCAGG EcoO109I 884 AGGTCCT [GA]G^G[ACGT]CC[CT] 4857 AGGCCCT EcoT22I 1760 ATGCAT ATGCA^T 2996 ATGCAT FbaI 1794 TGATCA T^GATCA 2205 TGATCA HgaI 324 GACGC GACGC(5/10) 58 GCGTC HpaI 730 GTTAAC GTT^AAC 4842 GTTAAC MamI 5608 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5963 GATCTTAATC MfeI 2644 CAATTG C^AATTG 5621 CAATTG MunI 2644 CAATTG C^AATTG 5621 CAATTG NsiI 1760 ATGCAT ATGCA^T 2996 ATGCAT NspV 1928 TTCGAA TT^CGAA 4646 TTCGAA PflMI 170 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2835 CCAAGAAGTGG PshBI 3466 ATTAAT AT^TAAT 6456 ATTAAT PvuII 2978 CAGCTG CAG^CTG 5306 CAGCTG ScaI 3570 AGTACT AGT^ACT 3736 AGTACT SfuI 1928 TTCGAA TT^CGAA 4646 TTCGAA Van91I 170 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2835 CCAAGAAGTGG XcmI 3197 CCATTTCCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3851 CCACCACTCAGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI BanI BanII BbeI BbsI BbvI BciVI BcnI BfaI BglII BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI EaeI EarI EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeI NdeII NgoMIV NlaIII NlaIV NspI PleI PstI RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII