##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI AspEI AviII AvrII BamHI
BbeI BbrPI BglII BlnI BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspHI BsrFI
BssHII Bst1107I BstBI BstUI BstZ17I
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoT22I
FseI FspI HgaI Hin1I KasI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RcaI
RsrII SacII SalI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspI SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 1981 GGTACC G^GTACC
AclI 3164 AACGTT AA^CGTT
AflII 1042 CTTAAG C^TTAAG
AflIII 340 ACATGT A^C[GA][CT]GT
Asp718I 1981 GGTACC G^GTACC
AspI 1570 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3684 CCCGAG C^[CT]CG[GA]G
BcgI 2714 GCAAGAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4478 TGATCA T^GATCA
BfrI 1042 CTTAAG C^TTAAG
BglI 3553 GCCTTTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 2523 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 3684 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2256 TGTACA T^GTACA
BspLU11I 340 ACATGT A^CATGT
BsrGI 2256 TGTACA T^GTACA
BstAPI 985 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 814 GGATCT [GA]^GATC[CT]
DraI 356 TTTAAA TTT^AAA
EcoRI 2366 GAATTC G^AATTC
EcoRV 3258 GATATC GAT^ATC
FbaI 4478 TGATCA T^GATCA
HaeII 1137 GGCGCT [GA]GCGC^[CT]
HincII 4132 GTTAAC GT[CT]^[GA]AC
HindII 4132 GTTAAC GT[CT]^[GA]AC
HindIII 1072 AAGCTT A^AGCTT
HpaI 4132 GTTAAC GTT^AAC
KpnI 1981 GGTACC GGTAC^C
MamI 2523 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 814 GGATCT [GA]^GATC[CT]
NcoI 611 CCATGG C^CATGG
PflFI 1570 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 3164 AACGTT AA^CGTT
SapI 3051 GCTCTTC GCTCTTC(1/4)
ScaI 2249 AGTACT AGT^ACT
SspBI 2256 TGTACA T^GTACA
Tth111I 1570 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1240 TCTAGA T^CTAGA
XhoII 814 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 530 GTAGAC GT^[AC][GT]AC
3759 GTCTAC
Alw44I 375 GTGCAC G^TGCAC
3021 GTGCAC
ApaLI 375 GTGCAC G^TGCAC
3021 GTGCAC
Asp700I 1511 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1600 GAAGGGTTTC
BciVI 3992 GGATAC GTATCC(6/5)
455 GTATCC
BlpI 2148 GCTTAGC GC^T[ACGT]AGC
3338 GCTCAGC
Bpu1102I 2148 GCTTAGC GC^T[ACGT]AGC
3338 GCTCAGC
BsrBI 2845 CCGCTC CCGCTC(-3/-3)
1920 GAGCGG
BsrDI 871 CATTGC GCAATG(2/0)
1844 CATTGC
BssSI 96 CTCGTG CACGAG(-5/-1)
1700 CTCGTG
BstEII 1195 GGTCACC G^GT[ACGT]ACC
3897 GGTGACC
BstPI 1195 GGTCACC G^GT[ACGT]ACC
3897 GGTGACC
CelII 2148 GCTTAGC GC^T[ACGT]AGC
3338 GCTCAGC
DraIII 2634 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2781 CACAGGGTG
EcoNI 1580 CCTGGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3565 CCTCCTGGAGG
EcoO65I 1195 GGTCACC G^GT[ACGT]ACC
3897 GGTGACC
FauI 3726 CCCGC CCCGC(4/6)
7 GCGGG
NspI 340 ACATGT [GA]CATG^[CT]
2623 GCATGT
SacI 2852 GAGCTC GAGCT^C
3916 GAGCTC
SbfI 1859 CCTGCAGG CCTGCA^GG
3810 CCTGCAGG
SexAI 801 ACCTGGT A^CC[AT]GGT
3016 ACCAGGT
Sse8387I 1859 CCTGCAGG CCTGCA^GG
3810 CCTGCAGG
TaqI 2204 TCGA T^CGA
3314 TCGA
TfiI 1516 GATTC G^A[AT]TC
2206 GATTC
TthHB8I 2204 TCGA T^CGA
3314 TCGA
XmnI 1511 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1600 GAAGGGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XcmI