##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AscI Asp718I AspEI
AspI AvrII BciVI BfrI BlnI
BsaWI BseAI BsiWI BspDI BspEI
BstBI ClaI CpoI Eam1105I EcoRI
EcoRV FseI HpaI KpnI MfeI
MluI MroI MunI NaeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PinAI PmeI Psp1406I PvuI
RsrII SalI SfuI SgrAI SmaI
SnaBI SphI SspI SwaI Tth111I
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 3082 GTATAC GT^[AC][GT]AC
AflIII 1897 ACATGT A^C[GA][CT]GT
Aor51HI 157 AGCGCT AGC^GCT
Asp700I 2058 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 3263 GGATCC G^GATCC
BglII 3246 AGATCT A^GATCT
BsaBI 3307 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 195 CGGCCG CG[GA][CT]^CG
BsmBI 1034 CGTCTC CGTCTC(1/5)
BsmI 1004 GCATTC GAATGC(1/-1)
Bsp1407I 4523 TGTACA T^GTACA
BspHI 952 TCATGA T^CATGA
BspLU11I 1897 ACATGT A^CATGT
BsrFI 2150 ACCGGC [GA]^CCGG[CT]
BsrGI 4523 TGTACA T^GTACA
BssHII 190 GCGCGC G^CGCGC
BssSI 484 CACGAG CACGAG(-5/-1)
Bst1107I 3082 GTATAC GTA^TAC
BstZ17I 3082 GTATAC GTA^TAC
Cfr10I 2150 ACCGGC [GA]^CCGG[CT]
EagI 195 CGGCCG C^GGCCG
EclXI 195 CGGCCG C^GGCCG
Eco47III 157 AGCGCT AGC^GCT
Eco52I 195 CGGCCG C^GGCCG
EcoNI 2307 CCTTTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 3385 ATGCAT ATGCA^T
HincII 3282 GTCAAC GT[CT]^[GA]AC
HindII 3282 GTCAAC GT[CT]^[GA]AC
HindIII 580 AAGCTT A^AGCTT
KspI 1954 CCGCGG CCGC^GG
MamI 3307 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 1690 CATATG CA^TATG
NsiI 3385 ATGCAT ATGCA^T
PshAI 2842 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 952 TCATGA T^CATGA
SacII 1954 CCGCGG CCGC^GG
SbfI 2439 CCTGCAGG CCTGCA^GG
SexAI 635 ACCAGGT A^CC[AT]GGT
SfiI 3851 GGCCACTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 2439 CCTGCAGG CCTGCA^GG
SspBI 4523 TGTACA T^GTACA
XmnI 2058 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Alw44I 481 GTGCAC G^TGCAC
2101 GTGCAC
ApaLI 481 GTGCAC G^TGCAC
2101 GTGCAC
AseI 2988 ATTAAT AT^TAAT
4421 ATTAAT
AsnI 2988 ATTAAT AT^TAAT
4421 ATTAAT
BbeI 16 GGCGCC GGCGC^C
4187 GGCGCC
BbrPI 1988 CACGTG CAC^GTG
2095 CACGTG
BcgI 340 GCAAAGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4122 GCATCATCCTCG
BclI 2393 TGATCA T^GATCA
3540 TGATCA
BlpI 185 GCTGAGC GC^T[ACGT]AGC
3686 GCTCAGC
Bpu1102I 185 GCTGAGC GC^T[ACGT]AGC
3686 GCTCAGC
BsaAI 1988 CACGTG [CT]AC^GT[GA]
2095 CACGTG
BsrBI 1047 CCGCTC CCGCTC(-3/-3)
1864 CCGCTC
BsrDI 1413 CATTGC GCAATG(2/0)
3225 CATTGC
BstAPI 130 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2129 GCAGCACCTGC
CelII 185 GCTGAGC GC^T[ACGT]AGC
3686 GCTCAGC
DraI 2748 TTTAAA TTT^AAA
4566 TTTAAA
DrdI 3890 GACAACAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4408 GACACCACAGTC
FbaI 2393 TGATCA T^GATCA
3540 TGATCA
KasI 16 GGCGCC G^GCGCC
4187 GGCGCC
NarI 16 GGCGCC GG^CGCC
4187 GGCGCC
NspI 1807 GCATGT [GA]CATG^[CT]
1897 ACATGT
PaeR7I 607 CTCGAG C^TCGAG
2076 CTCGAG
PflMI 798 CCACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3990 CCAGGCTGTGG
PmaCI 1988 CACGTG CAC^GTG
2095 CACGTG
PmlI 1988 CACGTG CAC^GTG
2095 CACGTG
PshBI 2988 ATTAAT AT^TAAT
4421 ATTAAT
SapI 1711 GAAGAGC GCTCTTC(1/4)
4299 GCTCTTC
ScaI 1253 AGTACT AGT^ACT
2072 AGTACT
SfoI 16 GGCGCC GGC^GCC
4187 GGCGCC
SpeI 1450 ACTAGT A^CTAGT
2661 ACTAGT
TaqI 608 TCGA T^CGA
2077 TCGA
TthHB8I 608 TCGA T^CGA
2077 TCGA
Van91I 798 CCACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3990 CCAGGCTGTGG
XhoI 607 CTCGAG C^TCGAG
2076 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII AviII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII