##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AgeI AhdI Alw44I ApaLI
AscI AspEI AspHI AvrII BalI
BbsI BclI BlnI BpuAI BsaBI
BsiEI BsiHKAI BsiWI BsmI BspDI
BsrBI BsrFI BssSI BstBI BstEII
BstPI Cfr10I ClaI CpoI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco52I EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI KspI MamI MluI
MluNI MscI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfuI
SgrAI SnaBI SphI Sse8387I SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
Acc65I 1263 GGTACC G^GTACC
AccI 1536 GTATAC GT^[AC][GT]AC
AccIII 443 TCCGGA T^CCGGA
AcyI 2798 GGCGCC G[GA]^CG[CT]C
AflIII 3334 ACATGT A^C[GA][CT]GT
AlwNI 300 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3128 CCTAAGG CC^T[ACGT]AGG
Aor51HI 2323 AGCGCT AGC^GCT
ApaI 36 GGGCCC GGGCC^C
Asp718I 1263 GGTACC G^GTACC
AspI 2676 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 977 TGCGCA TGC^GCA
BbeI 2798 GGCGCC GGCGC^C
BbrPI 203 CACGTG CAC^GTG
BbvCI 2326 GCTGAGG CCTCAGC(-5/-2)
BcgI 44 GCAGCACGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2108 GTATCC GTATCC(6/5)
BglI 778 GCCTGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1043 AGATCT A^GATCT
BsaHI 2798 GGCGCC G[GA]^CG[CT]C
BseAI 443 TCCGGA T^CCGGA
BseRI 273 GAGGAG GAGGAG(10/8)
BspEI 443 TCCGGA T^CCGGA
BspHI 2890 TCATGA T^CATGA
BspLU11I 3334 ACATGT A^CATGT
BspMI 2503 GCAGGT ACCTGC(4/8)
BsrDI 1328 CATTGC GCAATG(2/0)
BssHII 64 GCGCGC G^CGCGC
Bst1107I 1536 GTATAC GTA^TAC
BstXI 2914 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1536 GTATAC GTA^TAC
Bsu36I 3128 CCTAAGG CC^T[ACGT]AGG
Eco47III 2323 AGCGCT AGC^GCT
Eco81I 3128 CCTAAGG CC^T[ACGT]AGG
EcoRI 2293 GAATTC G^AATTC
EcoT14I 2696 CCAAGG C^C[AT][AT]GG
FspI 977 TGCGCA TGC^GCA
Hin1I 2798 GGCGCC G[GA]^CG[CT]C
HincII 2201 GTTAAC GT[CT]^[GA]AC
HindII 2201 GTTAAC GT[CT]^[GA]AC
HpaI 2201 GTTAAC GTT^AAC
KasI 2798 GGCGCC G^GCGCC
KpnI 1263 GGTACC GGTAC^C
MfeI 1476 CAATTG C^AATTG
MroI 443 TCCGGA T^CCGGA
MslI 585 CACCCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1476 CAATTG C^AATTG
NarI 2798 GGCGCC GG^CGCC
PflFI 2676 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 203 CACGTG CAC^GTG
PmlI 203 CACGTG CAC^GTG
RcaI 2890 TCATGA T^CATGA
SapI 353 GAAGAGC GCTCTTC(1/4)
ScaI 1486 AGTACT AGT^ACT
SfoI 2798 GGCGCC GGC^GCC
SmaI 126 CCCGGG CCC^GGG
SpeI 2983 ACTAGT A^CTAGT
StyI 2696 CCAAGG C^C[AT][AT]GG
Tth111I 2676 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1085 TCTAGA T^CTAGA
XcmI 420 CCAGGTGTTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 126 CCCGGG C^CCGGG
XmaI 126 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 63 CGCG CG^CG
65 CGCG
AclI 3 AACGTT AA^CGTT
186 AACGTT
AseI 1305 ATTAAT AT^TAAT
2256 ATTAAT
AsnI 1305 ATTAAT AT^TAAT
2256 ATTAAT
AvaI 39 CCCGAG C^[CT]CG[GA]G
126 CCCGGG
BamHI 800 GGATCC G^GATCC
3252 GGATCC
BanI 1263 GGTACC G^G[CT][GA]CC
2798 GGCGCC
BlpI 14 GCTTAGC GC^T[ACGT]AGC
896 GCTAAGC
Bpu1102I 14 GCTTAGC GC^T[ACGT]AGC
896 GCTAAGC
BsaAI 111 CACGTA [CT]AC^GT[GA]
203 CACGTG
BsaWI 443 TCCGGA [AT]^CCGG[AT]
3255 TCCGGT
BsgI 582 CTGCAC GTGCAG(16/14)
2707 GTGCAG
BsmBI 2674 GAGACG CGTCTC(1/5)
2842 GAGACG
BsoBI 39 CCCGAG C^[CT]CG[GA]G
126 CCCGGG
Bsp1407I 689 TGTACA T^GTACA
962 TGTACA
BsrGI 689 TGTACA T^GTACA
962 TGTACA
BstUI 63 CGCG CG^CG
65 CGCG
CelII 14 GCTTAGC GC^T[ACGT]AGC
896 GCTAAGC
DsaI 339 CCACGG C^C[GA][CT]GG
737 CCACGG
FauI 19 GCGGG CCCGC(4/6)
74 GCGGG
HaeII 2323 AGCGCT [GA]GCGC^[CT]
2798 GGCGCC
HindIII 1598 AAGCTT A^AGCTT
1747 AAGCTT
MvnI 63 CGCG CG^CG
65 CGCG
PpuMI 2209 GGGTCCT [GA]G^G[AT]CC[CT]
2575 GGGACCT
PshBI 1305 ATTAAT AT^TAAT
2256 ATTAAT
Psp1406I 3 AACGTT AA^CGTT
186 AACGTT
PstI 99 CTGCAG CTGCA^G
885 CTGCAG
PvuII 902 CAGCTG CAG^CTG
1002 CAGCTG
SspBI 689 TGTACA T^GTACA
962 TGTACA
TaqI 407 TCGA T^CGA
708 TCGA
TthHB8I 407 TCGA T^CGA
708 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI ApoI Asp700I AvaII BanII
BbvI BcnI BfaI BfrI BmyI
BpmI BsaI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XhoII XmnI