##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII BamHI BbrPI BcgI BglI
BsaAI BsaBI BseAI BsiWI BsmBI
BspDI BspEI BsrFI BssHII BstBI
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV FseI FspI HindIII HpaI
KpnI MamI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccII 141 CGCG CG^CG
AflII 4443 CTTAAG C^TTAAG
AspI 2369 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 3168 CCTCAGC CCTCAGC(-5/-2)
BciVI 840 GTATCC GTATCC(6/5)
BfrI 4443 CTTAAG C^TTAAG
BglII 4293 AGATCT A^GATCT
Bsp1407I 1192 TGTACA T^GTACA
BsrDI 2531 CATTGC GCAATG(2/0)
BsrGI 1192 TGTACA T^GTACA
BssSI 1322 CTCGTG CACGAG(-5/-1)
BstAPI 3308 GCATTACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3149 GGTTACC G^GT[ACGT]ACC
BstPI 3149 GGTTACC G^GT[ACGT]ACC
BstUI 141 CGCG CG^CG
BstXI 1263 CCAAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoNI 1655 CCTGGTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3149 GGTTACC G^GT[ACGT]ACC
EcoT22I 3306 ATGCAT ATGCA^T
KspI 140 CCGCGG CCGC^GG
MaeII 1001 ACGT A^CGT
MvnI 141 CGCG CG^CG
NheI 1670 GCTAGC G^CTAGC
NsiI 3306 ATGCAT ATGCA^T
PflFI 2369 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 140 CCGCGG CCGC^GG
SalI 364 GTCGAC G^TCGAC
SapI 2521 GCTCTTC GCTCTTC(1/4)
SbfI 1031 CCTGCAGG CCTGCA^GG
SexAI 1654 ACCTGGT A^CC[AT]GGT
Sse8387I 1031 CCTGCAGG CCTGCA^GG
SspBI 1192 TGTACA T^GTACA
Tth111I 2369 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3120 CCAAGGCCTAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AspHI 2906 GAGCAC G[AT]GC[AT]^C
4093 GAGCAC
AvaI 63 CTCGGG C^[CT]CG[GA]G
4130 CTCGGG
BbeI 153 GGCGCC GGCGC^C
170 GGCGCC
BlpI 160 GCTGAGC GC^T[ACGT]AGC
4303 GCTTAGC
Bpu1102I 160 GCTGAGC GC^T[ACGT]AGC
4303 GCTTAGC
BsaWI 360 TCCGGT [AT]^CCGG[AT]
3259 ACCGGA
BsiEI 362 CGGTCG CG[GA][CT]^CG
366 CGACCG
BsiHKAI 2906 GAGCAC G[AT]GC[AT]^C
4093 GAGCAC
BsoBI 63 CTCGGG C^[CT]CG[GA]G
4130 CTCGGG
BsrBI 436 CCGCTC CCGCTC(-3/-3)
3564 CCGCTC
Bst1107I 2473 GTATAC GTA^TAC
4267 GTATAC
BstYI 1702 AGATCC [GA]^GATC[CT]
4293 AGATCT
BstZ17I 2473 GTATAC GTA^TAC
4267 GTATAC
CelII 160 GCTGAGC GC^T[ACGT]AGC
4303 GCTTAGC
EcoRI 2649 GAATTC G^AATTC
3059 GAATTC
HaeII 153 GGCGCC [GA]GCGC^[CT]
170 GGCGCC
HgaI 281 GCGTC GACGC(5/10)
3951 GCGTC
HincII 364 GTCGAC GT[CT]^[GA]AC
3615 GTTGAC
HindII 364 GTCGAC GT[CT]^[GA]AC
3615 GTTGAC
KasI 153 GGCGCC G^GCGCC
170 GGCGCC
MflI 1702 AGATCC [GA]^GATC[CT]
4293 AGATCT
NarI 153 GGCGCC GG^CGCC
170 GGCGCC
SfoI 153 GGCGCC GGC^GCC
170 GGCGCC
SphI 257 GCATGC GCATG^C
620 GCATGC
SspI 1523 AATATT AAT^ATT
2176 AATATT
StuI 2863 AGGCCT AGG^CCT
3123 AGGCCT
XbaI 2029 TCTAGA T^CTAGA
3055 TCTAGA
XhoII 1702 AGATCC [GA]^GATC[CT]
4293 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI Asp700I AvaII AvrII BalI
BanI BanII BbsI BbvI BclI
BcnI BfaI BlnI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspHI BspLU11I BspMI
BsrI BssKI BstF5I BstNI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XmnI