##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BbeI
BbrPI BlnI BsaBI BsaI BsiEI
BsmBI BsoBI BspDI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraIII EagI Eam1105I EclXI Eco52I
EcoO65I EcoRV EcoT22I FseI FspI
HindIII KasI KpnI KspI MamI
MluI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccII 1961 CGCG CG^CG
AccIII 2542 TCCGGA T^CCGGA
AcyI 3875 GGCGTC G[GA]^CG[CT]C
Alw44I 1556 GTGCAC G^TGCAC
ApaLI 1556 GTGCAC G^TGCAC
BamHI 242 GGATCC G^GATCC
BcgI 1812 CGAGCTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2391 GTATCC GTATCC(6/5)
BclI 3911 TGATCA T^GATCA
BlpI 2782 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2782 GCTAAGC GC^T[ACGT]AGC
BsaHI 3875 GGCGTC G[GA]^CG[CT]C
BsaWI 2542 TCCGGA [AT]^CCGG[AT]
BseAI 2542 TCCGGA T^CCGGA
BsiWI 3078 CGTACG C^GTACG
Bsp1407I 137 TGTACA T^GTACA
BspEI 2542 TCCGGA T^CCGGA
BspLU11I 4394 ACATGT A^CATGT
BsrGI 137 TGTACA T^GTACA
BssHII 1960 GCGCGC G^CGCGC
BstUI 1961 CGCG CG^CG
CelII 2782 GCTAAGC GC^T[ACGT]AGC
FbaI 3911 TGATCA T^GATCA
Hin1I 3875 GGCGTC G[GA]^CG[CT]C
HpaI 2914 GTTAAC GTT^AAC
MfeI 133 CAATTG C^AATTG
MroI 2542 TCCGGA T^CCGGA
MunI 133 CAATTG C^AATTG
MvnI 1961 CGCG CG^CG
NheI 1807 GCTAGC G^CTAGC
SbfI 2881 CCTGCAGG CCTGCA^GG
ScaI 1321 AGTACT AGT^ACT
SfiI 3566 GGCCGTACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 2881 CCTGCAGG CCTGCA^GG
SspBI 137 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 228 GTCTAC GT^[AC][GT]AC
3305 GTCTAC
AflII 3154 CTTAAG C^TTAAG
3737 CTTAAG
AflIII 3309 ACGTGT A^C[GA][CT]GT
4394 ACATGT
Aor51HI 3227 AGCGCT AGC^GCT
3942 AGCGCT
ApaI 3073 GGGCCC GGGCC^C
3581 GGGCCC
BbvCI 3129 CCTCAGC CCTCAGC(-5/-2)
4242 CCTCAGC
BcnI 484 CCCGG CC^[GC]GG
2991 CCGGG
BfrI 3154 CTTAAG C^TTAAG
3737 CTTAAG
BglII 977 AGATCT A^GATCT
2450 AGATCT
BsgI 1904 CTGCAC GTGCAG(16/14)
3245 CTGCAC
BspHI 2963 TCATGA T^CATGA
3036 TCATGA
BspMI 2880 ACCTGC ACCTGC(4/8)
3243 ACCTGC
BsrBI 1953 CCGCTC CCGCTC(-3/-3)
4009 CCGCTC
BsrFI 380 ACCGGC [GA]^CCGG[CT]
1786 ACCGGC
BssSI 416 CACGAG CACGAG(-5/-1)
3500 CACGAG
BstAPI 685 GCATGTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3238 GCATCACCTGC
Cfr10I 380 ACCGGC [GA]^CCGG[CT]
1786 ACCGGC
DraI 21 TTTAAA TTT^AAA
1500 TTTAAA
DrdI 2062 GACTCAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4188 GACAGGGGAGTC
Eco47III 3227 AGCGCT AGC^GCT
3942 AGCGCT
EcoRI 1287 GAATTC G^AATTC
4094 GAATTC
HaeII 3227 AGCGCT [GA]GCGC^[CT]
3942 AGCGCT
HgaI 2829 GACGC GACGC(5/10)
3876 GCGTC
HincII 2914 GTTAAC GT[CT]^[GA]AC
3742 GTTGAC
HindII 2914 GTTAAC GT[CT]^[GA]AC
3742 GTTGAC
NciI 484 CCCGG CC^[GC]GG
2991 CCGGG
NcoI 1386 CCATGG C^CATGG
2597 CCATGG
PpuMI 534 GGGACCT [GA]G^G[AT]CC[CT]
2655 AGGACCC
RcaI 2963 TCATGA T^CATGA
3036 TCATGA
SacI 1813 GAGCTC GAGCT^C
3331 GAGCTC
SphI 4293 GCATGC GCATG^C
4429 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI Asp700I AspHI
AvaII BalI BanI BanII BbsI
BbvI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI