################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII Acc65I AclI AgeI AhdI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BbeI BbrPI BlnI BsaBI BsaI BsiEI BsmBI BsoBI BspDI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraIII EagI Eam1105I EclXI Eco52I EcoO65I EcoRV EcoT22I FseI FspI HindIII KasI KpnI KspI MamI MluI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccII 1961 CGCG CG^CG AccIII 2542 TCCGGA T^CCGGA AcyI 3875 GGCGTC G[GA]^CG[CT]C Alw44I 1556 GTGCAC G^TGCAC ApaLI 1556 GTGCAC G^TGCAC BamHI 242 GGATCC G^GATCC BcgI 1812 CGAGCTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2391 GTATCC GTATCC(6/5) BclI 3911 TGATCA T^GATCA BlpI 2782 GCTAAGC GC^T[ACGT]AGC Bpu1102I 2782 GCTAAGC GC^T[ACGT]AGC BsaHI 3875 GGCGTC G[GA]^CG[CT]C BsaWI 2542 TCCGGA [AT]^CCGG[AT] BseAI 2542 TCCGGA T^CCGGA BsiWI 3078 CGTACG C^GTACG Bsp1407I 137 TGTACA T^GTACA BspEI 2542 TCCGGA T^CCGGA BspLU11I 4394 ACATGT A^CATGT BsrGI 137 TGTACA T^GTACA BssHII 1960 GCGCGC G^CGCGC BstUI 1961 CGCG CG^CG CelII 2782 GCTAAGC GC^T[ACGT]AGC FbaI 3911 TGATCA T^GATCA Hin1I 3875 GGCGTC G[GA]^CG[CT]C HpaI 2914 GTTAAC GTT^AAC MfeI 133 CAATTG C^AATTG MroI 2542 TCCGGA T^CCGGA MunI 133 CAATTG C^AATTG MvnI 1961 CGCG CG^CG NheI 1807 GCTAGC G^CTAGC SbfI 2881 CCTGCAGG CCTGCA^GG ScaI 1321 AGTACT AGT^ACT SfiI 3566 GGCCGTACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 2881 CCTGCAGG CCTGCA^GG SspBI 137 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 228 GTCTAC GT^[AC][GT]AC 3305 GTCTAC AflII 3154 CTTAAG C^TTAAG 3737 CTTAAG AflIII 3309 ACGTGT A^C[GA][CT]GT 4394 ACATGT Aor51HI 3227 AGCGCT AGC^GCT 3942 AGCGCT ApaI 3073 GGGCCC GGGCC^C 3581 GGGCCC BbvCI 3129 CCTCAGC CCTCAGC(-5/-2) 4242 CCTCAGC BcnI 484 CCCGG CC^[GC]GG 2991 CCGGG BfrI 3154 CTTAAG C^TTAAG 3737 CTTAAG BglII 977 AGATCT A^GATCT 2450 AGATCT BsgI 1904 CTGCAC GTGCAG(16/14) 3245 CTGCAC BspHI 2963 TCATGA T^CATGA 3036 TCATGA BspMI 2880 ACCTGC ACCTGC(4/8) 3243 ACCTGC BsrBI 1953 CCGCTC CCGCTC(-3/-3) 4009 CCGCTC BsrFI 380 ACCGGC [GA]^CCGG[CT] 1786 ACCGGC BssSI 416 CACGAG CACGAG(-5/-1) 3500 CACGAG BstAPI 685 GCATGTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3238 GCATCACCTGC Cfr10I 380 ACCGGC [GA]^CCGG[CT] 1786 ACCGGC DraI 21 TTTAAA TTT^AAA 1500 TTTAAA DrdI 2062 GACTCAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4188 GACAGGGGAGTC Eco47III 3227 AGCGCT AGC^GCT 3942 AGCGCT EcoRI 1287 GAATTC G^AATTC 4094 GAATTC HaeII 3227 AGCGCT [GA]GCGC^[CT] 3942 AGCGCT HgaI 2829 GACGC GACGC(5/10) 3876 GCGTC HincII 2914 GTTAAC GT[CT]^[GA]AC 3742 GTTGAC HindII 2914 GTTAAC GT[CT]^[GA]AC 3742 GTTGAC NciI 484 CCCGG CC^[GC]GG 2991 CCGGG NcoI 1386 CCATGG C^CATGG 2597 CCATGG PpuMI 534 GGGACCT [GA]G^G[AT]CC[CT] 2655 AGGACCC RcaI 2963 TCATGA T^CATGA 3036 TCATGA SacI 1813 GAGCTC GAGCT^C 3331 GAGCTC SphI 4293 GCATGC GCATG^C 4429 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaII BalI BanI BanII BbsI BbvI BfaI BglI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI