##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflIII AgeI AhdI AocI Aor51HI
ApaI AscI Asp718I AspEI AspI
AvaI AviII AvrII BanI BbeI
BbrPI BclI BglI BlnI BsaAI
BsaHI BsiEI BsiWI BsmBI BsoBI
BspDI BspHI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I FbaI FseI FspI HgaI
Hin1I HpaI KasI KpnI KspI
MluI MvnI NaeI NarI NgoMIV
NotI NruI NspV PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
Van91I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 1626 GTCTAC GT^[AC][GT]AC
AccIII 2946 TCCGGA T^CCGGA
Alw44I 2190 GTGCAC G^TGCAC
ApaLI 2190 GTGCAC G^TGCAC
AspHI 2190 GTGCAC G[AT]GC[AT]^C
BcgI 3057 GCATGTGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1969 GTATCC GTATCC(6/5)
BcnI 1843 CCGGG CC^[GC]GG
BglII 3172 AGATCT A^GATCT
BsaBI 2558 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1952 GAGACC GGTCTC(1/5)
BsaWI 2946 TCCGGA [AT]^CCGG[AT]
BseAI 2946 TCCGGA T^CCGGA
BsiHKAI 2190 GTGCAC G[AT]GC[AT]^C
BspEI 2946 TCCGGA T^CCGGA
BstAPI 1661 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 2167 GCGC GCG^C
EcoNI 1376 CCTTTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2375 GATATC GAT^ATC
FauI 60 CCCGC CCCGC(4/6)
HaeII 2166 AGCGCC [GA]GCGC^[CT]
HhaI 2167 GCGC GCG^C
HinP1I 2167 GCGC G^CGC
HincII 263 GTTGAC GT[CT]^[GA]AC
HindII 263 GTTGAC GT[CT]^[GA]AC
HindIII 770 AAGCTT A^AGCTT
MamI 2558 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2946 TCCGGA T^CCGGA
NciI 1843 CCGGG CC^[GC]GG
NdeI 3162 CATATG CA^TATG
NheI 2290 GCTAGC G^CTAGC
PacI 3852 TTAATTAA TTAAT^TAA
PpuMI 3290 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1022 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1534 GAAGAGC GCTCTTC(1/4)
SexAI 3564 ACCAGGT A^CC[AT]GGT
StuI 1384 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflII 4 CTTAAG C^TTAAG
323 CTTAAG
AlwNI 3641 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
3752 CAGAGACTG
BalI 2073 TGGCCA TGG^CCA
3441 TGGCCA
BamHI 1201 GGATCC G^GATCC
3969 GGATCC
BbvCI 22 CCTCAGC CCTCAGC(-5/-2)
2333 CCTCAGC
BfrI 4 CTTAAG C^TTAAG
323 CTTAAG
BlpI 656 GCTCAGC GC^T[ACGT]AGC
3309 GCTTAGC
Bpu1102I 656 GCTCAGC GC^T[ACGT]AGC
3309 GCTTAGC
BsgI 3006 CTGCAC GTGCAG(16/14)
2853 GTGCAG
Bsp1407I 465 TGTACA T^GTACA
2140 TGTACA
BspMI 3453 ACCTGC ACCTGC(4/8)
3607 GCAGGT
BsrGI 465 TGTACA T^GTACA
2140 TGTACA
BstXI 779 CCACCGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3538 CCAGGATTATGG
CelII 656 GCTCAGC GC^T[ACGT]AGC
3309 GCTTAGC
DsaI 1264 CCATGG C^C[GA][CT]GG
2775 CCATGG
EaeI 2073 TGGCCA [CT]^GGCC[GA]
3441 TGGCCA
HapII 1843 CCGG C^CGG
2947 CCGG
HpaII 1843 CCGG C^CGG
2947 CCGG
MaeII 191 ACGT A^CGT
2748 ACGT
MfeI 2099 CAATTG C^AATTG
3107 CAATTG
MluNI 2073 TGGCCA TGG^CCA
3441 TGGCCA
MscI 2073 TGGCCA TGG^CCA
3441 TGGCCA
MspI 1843 CCGG C^CGG
2947 CCGG
MunI 2099 CAATTG C^AATTG
3107 CAATTG
NcoI 1264 CCATGG C^CATGG
2775 CCATGG
PleI 619 GAGTC GAGTC(4/5)
2224 GAGTC
SspBI 465 TGTACA T^GTACA
2140 TGTACA
XbaI 431 TCTAGA T^CTAGA
1075 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI Asp700I AvaII
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI