##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
Alw44I AlwNI ApaLI AscI AspEI
AvrII BbvCI BcgI BfrI BglII
BlnI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrGI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoRV FseI HpaI MluI MroI
NdeI NheI NotI NruI NspV
PacI PinAI PmeI PshAI PvuI
RsrII SalI SbfI ScaI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 938 GGTACC G^GTACC
AclI 4751 AACGTT AA^CGTT
AflIII 1800 ACGTGT A^C[GA][CT]GT
AocI 3836 CCTCAGG CC^T[ACGT]AGG
Aor51HI 4637 AGCGCT AGC^GCT
AseI 542 ATTAAT AT^TAAT
AsnI 542 ATTAAT AT^TAAT
Asp700I 1810 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 938 GGTACC G^GTACC
AviII 2043 TGCGCA TGC^GCA
BalI 4304 TGGCCA TGG^CCA
BbrPI 2825 CACGTG CAC^GTG
BciVI 736 GTATCC GTATCC(6/5)
BclI 3542 TGATCA T^GATCA
BglI 4107 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 248 TCCGGT [AT]^CCGG[AT]
BsmI 958 GAATGC GAATGC(1/-1)
BspHI 3428 TCATGA T^CATGA
BsrBI 755 GAGCGG CCGCTC(-3/-3)
BssHII 216 GCGCGC G^CGCGC
BstAPI 2821 GCAGCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3836 CCTCAGG CC^T[ACGT]AGG
DraIII 2381 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 4637 AGCGCT AGC^GCT
Eco81I 3836 CCTCAGG CC^T[ACGT]AGG
EcoT22I 1632 ATGCAT ATGCA^T
FbaI 3542 TGATCA T^GATCA
FspI 2043 TGCGCA TGC^GCA
HincII 43 GTCAAC GT[CT]^[GA]AC
HindII 43 GTCAAC GT[CT]^[GA]AC
KpnI 938 GGTACC GGTAC^C
MfeI 172 CAATTG C^AATTG
MluNI 4304 TGGCCA TGG^CCA
MscI 4304 TGGCCA TGG^CCA
MunI 172 CAATTG C^AATTG
NaeI 2774 GCCGGC GCC^GGC
NgoMIV 2774 GCCGGC G^CCGGC
NsiI 1632 ATGCAT ATGCA^T
PaeR7I 2198 CTCGAG C^TCGAG
PmaCI 2825 CACGTG CAC^GTG
PmlI 2825 CACGTG CAC^GTG
PshBI 542 ATTAAT AT^TAAT
Psp1406I 4751 AACGTT AA^CGTT
RcaI 3428 TCATGA T^CATGA
SapI 2458 GAAGAGC GCTCTTC(1/4)
SfiI 4106 GGCCAGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 333 CCCGGG CCC^GGG
SphI 3666 GCATGC GCATG^C
XhoI 2198 CTCGAG C^TCGAG
XmaCI 333 CCCGGG C^CCGGG
XmaI 333 CCCGGG C^CCGGG
XmnI 1810 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 2849 GTCTAC GT^[AC][GT]AC
3227 GTAGAC
ApaI 759 GGGCCC GGGCC^C
4208 GGGCCC
AspHI 446 GAGCTC G[AT]GC[AT]^C
2752 GAGCTC
BamHI 245 GGATCC G^GATCC
2166 GGATCC
BbeI 1660 GGCGCC GGCGC^C
1897 GGCGCC
BsaBI 1492 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3543 GATCACCATC
BsgI 2027 CTGCAC GTGCAG(16/14)
3028 CTGCAC
BsiHKAI 446 GAGCTC G[AT]GC[AT]^C
2752 GAGCTC
BsrDI 2865 GCAATG GCAATG(2/0)
2922 GCAATG
BsrFI 2669 ACCGGC [GA]^CCGG[CT]
2774 GCCGGC
BssSI 2429 CACGAG CACGAG(-5/-1)
2090 CTCGTG
BstEII 418 GGTCACC G^GT[ACGT]ACC
1927 GGTGACC
BstPI 418 GGTCACC G^GT[ACGT]ACC
1927 GGTGACC
Cfr10I 2669 ACCGGC [GA]^CCGG[CT]
2774 GCCGGC
EcoNI 1987 CCTGCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2335 CCTGCTGCAGG
EcoO65I 418 GGTCACC G^GT[ACGT]ACC
1927 GGTGACC
EcoRI 135 GAATTC G^AATTC
397 GAATTC
HindIII 677 AAGCTT A^AGCTT
3788 AAGCTT
KasI 1660 GGCGCC G^GCGCC
1897 GGCGCC
MamI 1492 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3543 GATCACCATC
NarI 1660 GGCGCC GG^CGCC
1897 GGCGCC
SacI 446 GAGCTC GAGCT^C
2752 GAGCTC
SfoI 1660 GGCGCC GGC^GCC
1897 GGCGCC
StuI 3036 AGGCCT AGG^CCT
3811 AGGCCT
XbaI 1006 TCTAGA T^CTAGA
1084 TCTAGA
XcmI 3086 CCATGCTCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3205 CCATGGGGGTAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XhoII