##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AclI AgeI Aor51HI AscI
Asp718I AspI AvrII BbeI BcgI
BclI BlnI BsaBI BsiWI Bsp1407I
BspDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
ClaI Eco47III EcoO65I FbaI FseI
HindIII HpaI KasI KpnI KspI
MamI MluI NarI NdeI NheI
NotI NruI NspV PacI PflFI
PinAI PmeI PshAI Psp1406I PvuI
SacII SbfI SexAI SfiI SfoI
SfuI SpeI Sse8387I SspBI SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 865 GTCGAC GT^[AC][GT]AC
AccIII 2020 TCCGGA T^CCGGA
AflII 4796 CTTAAG C^TTAAG
AhdI 2369 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1921 CCTCAGG CC^T[ACGT]AGG
AspEI 2369 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2295 TGCGCA TGC^GCA
BamHI 3281 GGATCC G^GATCC
BbvCI 3104 GCTGAGG CCTCAGC(-5/-2)
BciVI 3511 GGATAC GTATCC(6/5)
BfrI 4796 CTTAAG C^TTAAG
BlpI 3401 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3401 GCTAAGC GC^T[ACGT]AGC
BseAI 2020 TCCGGA T^CCGGA
BsmI 3096 GCATTC GAATGC(1/-1)
BspEI 2020 TCCGGA T^CCGGA
BspLU11I 895 ACATGT A^CATGT
BstXI 753 CCAACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1921 CCTCAGG CC^T[ACGT]AGG
CelII 3401 GCTAAGC GC^T[ACGT]AGC
CpoI 1971 CGGTCCG CG^G[AT]CCG
DrdI 2651 GACAAAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2369 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1921 CCTCAGG CC^T[ACGT]AGG
EcoNI 2895 CCTTTTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 367 GAATTC G^AATTC
EcoRV 673 GATATC GAT^ATC
FspI 2295 TGCGCA TGC^GCA
MfeI 207 CAATTG C^AATTG
MroI 2020 TCCGGA T^CCGGA
MunI 207 CAATTG C^AATTG
PaeR7I 3030 CTCGAG C^TCGAG
PpuMI 3898 AGGTCCC [GA]G^G[AT]CC[CT]
RsrII 1971 CGGTCCG CG^G[AT]CCG
SalI 865 GTCGAC G^TCGAC
SapI 1759 GAAGAGC GCTCTTC(1/4)
SgrAI 2390 CACCGGCG C[GA]^CCGG[CT]G
SmaI 3277 CCCGGG CCC^GGG
SnaBI 330 TACGTA TAC^GTA
SspI 2996 AATATT AAT^ATT
StuI 2340 AGGCCT AGG^CCT
XhoI 3030 CTCGAG C^TCGAG
XmaCI 3277 CCCGGG C^CCGGG
XmaI 3277 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 824 GACGTC GACGT^C
3643 GACGTC
AflIII 895 ACATGT A^C[GA][CT]GT
1693 ACACGT
ApaI 1445 GGGCCC GGGCC^C
2107 GGGCCC
AseI 2228 ATTAAT AT^TAAT
4238 ATTAAT
AsnI 2228 ATTAAT AT^TAAT
4238 ATTAAT
BalI 2325 TGGCCA TGG^CCA
3256 TGGCCA
BanI 3158 GGCACC G^G[CT][GA]CC
4515 GGCACC
BglI 1889 GCCGGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3579 GCCACGATGGC
BglII 3663 AGATCT A^GATCT
3828 AGATCT
BsaWI 1831 ACCGGA [AT]^CCGG[AT]
2020 TCCGGA
BsmBI 3641 GAGACG CGTCTC(1/5)
4592 GAGACG
BsrBI 1952 CCGCTC CCGCTC(-3/-3)
2180 CCGCTC
DraIII 2528 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
3343 CACCCAGTG
EcoT22I 495 ATGCAT ATGCA^T
4630 ATGCAT
HaeII 1799 AGCGCC [GA]GCGC^[CT]
2100 GGCGCT
MluNI 2325 TGGCCA TGG^CCA
3256 TGGCCA
MscI 2325 TGGCCA TGG^CCA
3256 TGGCCA
NaeI 2097 GCCGGC GCC^GGC
2598 GCCGGC
NgoMIV 2097 GCCGGC G^CCGGC
2598 GCCGGC
NsiI 495 ATGCAT ATGCA^T
4630 ATGCAT
PshBI 2228 ATTAAT AT^TAAT
4238 ATTAAT
ScaI 2790 AGTACT AGT^ACT
4048 AGTACT
SphI 385 GCATGC GCATG^C
2244 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaLI
ApoI Asp700I AspHI AvaI AvaII
BanII BbrPI BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI