##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AviII BamHI BanI BanII
BbeI BbrPI BbsI BcgI BglI
BglII BlpI Bpu1102I BpuAI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BspDI BspEI BspMI BsrBI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV FseI FspI
HgaI Hin1I KasI KpnI KspI
MluI MroI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PvuI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I Van91I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccII 555 CGCG CG^CG
AclI 4725 AACGTT AA^CGTT
Asp700I 1767 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3780 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 3045 GGTCC G^G[AT]CC
AvrII 3124 CCTAGG C^CTAGG
BalI 3415 TGGCCA TGG^CCA
BciVI 2747 GTATCC GTATCC(6/5)
BcnI 3923 CCCGG CC^[GC]GG
BlnI 3124 CCTAGG C^CTAGG
BmyI 3677 GGGCAC G[AGT]GC[ACT]^C
BpmI 35 CTGGAG CTGGAG(16/14)
BsaAI 3594 TACGTG [CT]AC^GT[GA]
BsaBI 3981 GATCTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2257 GAGACC GGTCTC(1/5)
BsmBI 3951 GAGACG CGTCTC(1/5)
BsmFI 1825 GGGAC GGGAC(10/14)
BsmI 3452 GCATTC GAATGC(1/-1)
Bsp1286I 3677 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 2804 TGTACA T^GTACA
BsrGI 2804 TGTACA T^GTACA
BssSI 3998 CTCGTG CACGAG(-5/-1)
BstAPI 248 GCATTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 555 CGCG CG^CG
BstYI 3290 GGATCT [GA]^GATC[CT]
EaeI 3415 TGGCCA [CT]^GGCC[GA]
EarI 305 GAAGAG CTCTTC(1/4)
EcoRI 140 GAATTC G^AATTC
EcoT22I 770 ATGCAT ATGCA^T
FauI 4009 CCCGC CCCGC(4/6)
HaeII 13 GGCGCT [GA]GCGC^[CT]
HapII 3924 CCGG C^CGG
HindIII 3097 AAGCTT A^AGCTT
HpaI 1102 GTTAAC GTT^AAC
HpaII 3924 CCGG C^CGG
Ksp632I 305 GAAGAG CTCTTC(1/4)
MamI 3981 GATCTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 57 CAATTG C^AATTG
MflI 3290 GGATCT [GA]^GATC[CT]
MluNI 3415 TGGCCA TGG^CCA
MscI 3415 TGGCCA TGG^CCA
MspI 3924 CCGG C^CGG
MunI 57 CAATTG C^AATTG
MvnI 555 CGCG CG^CG
NciI 3923 CCCGG CC^[GC]GG
NsiI 770 ATGCAT ATGCA^T
PaeR7I 279 CTCGAG C^TCGAG
PflFI 3780 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3044 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1833 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4725 AACGTT AA^CGTT
SexAI 4238 ACCTGGT A^CC[AT]GGT
SfaNI 769 GATGC GCATC(5/9)
SspBI 2804 TGTACA T^GTACA
StuI 839 AGGCCT AGG^CCT
Tth111I 3780 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1495 CCAGTTATTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 279 CTCGAG C^TCGAG
XhoII 3290 GGATCT [GA]^GATC[CT]
XmnI 1767 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 1569 GTAGAC GT^[AC][GT]AC
4245 GTAGAC
AflII 4103 CTTAAG C^TTAAG
4136 CTTAAG
AlwI 2202 GGATC GGATC(4/5)
3290 GGATC
AvaI 279 CTCGAG C^[CT]CG[GA]G
3887 CCCGAG
BbvCI 3368 CCTCAGC CCTCAGC(-5/-2)
3878 CCTCAGC
BfrI 4103 CTTAAG C^TTAAG
4136 CTTAAG
BsiYI 3457 CCTTATTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3847 CCACCTCAAGG
BslI 3457 CCTTATTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3847 CCACCTCAAGG
BsoBI 279 CTCGAG C^[CT]CG[GA]G
3887 CCCGAG
BspLU11I 172 ACATGT A^CATGT
4261 ACATGT
CfoI 14 GCGC GCG^C
556 GCGC
HhaI 14 GCGC GCG^C
556 GCGC
HinP1I 14 GCGC G^CGC
556 GCGC
MspA1I 2443 CAGCTG C[AC]G^C[GT]G
2511 CAGCTG
PstI 2446 CTGCAG CTGCA^G
3341 CTGCAG
PvuII 2443 CAGCTG CAG^CTG
2511 CAGCTG
ScaI 4132 AGTACT AGT^ACT
4154 AGTACT
TaqI 280 TCGA T^CGA
3985 TCGA
TthHB8I 280 TCGA T^CGA
3985 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
ApoI AseI AsnI BbvI BclI
BfaI BsaJI BseRI BsgI BsmAI
BspHI BsrDI BsrI BssKI BstF5I
BstNI BstXI Cac8I Cfr13I DdeI
DpnII DraI DraII EcoO109I EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PshBI RcaI RsaI Sau3AI Sau96I
ScrFI SfcI SmlI SspI StyI
SwaI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI