##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I ApaLI AseI AsnI
AspEI AspI AvrII BbrPI BbvCI
BfrI BglI BlnI BseAI BsiWI
BspEI BstEII BstPI CpoI DraIII
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI HpaI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RsrII SalI ScaI SexAI
SfiI SgrAI SnaBI SpeI SphI
SwaI Tth111I Van91I XbaI XcmI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 2894 GGTACC G^GTACC
AccI 4355 GTATAC GT^[AC][GT]AC
AflIII 4351 ACATGT A^C[GA][CT]GT
Aor51HI 910 AGCGCT AGC^GCT
AscI 830 GGCGCGCC GG^CGCGCC
Asp718I 2894 GGTACC G^GTACC
AviII 1066 TGCGCA TGC^GCA
BamHI 2273 GGATCC G^GATCC
BclI 2518 TGATCA T^GATCA
BlpI 851 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 851 GCTCAGC GC^T[ACGT]AGC
BsaAI 1245 TACGTG [CT]AC^GT[GA]
BsaI 3500 GAGACC GGTCTC(1/5)
BsaWI 3348 TCCGGT [AT]^CCGG[AT]
BsgI 3886 CTGCAC GTGCAG(16/14)
Bsp1407I 4198 TGTACA T^GTACA
BspDI 2643 ATCGAT AT^CGAT
BspHI 610 TCATGA T^CATGA
BspLU11I 4351 ACATGT A^CATGT
BsrDI 3604 CATTGC GCAATG(2/0)
BsrFI 1198 ACCGGC [GA]^CCGG[CT]
BsrGI 4198 TGTACA T^GTACA
Bst1107I 4355 GTATAC GTA^TAC
BstXI 502 CCAACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4355 GTATAC GTA^TAC
CelII 851 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1198 ACCGGC [GA]^CCGG[CT]
ClaI 2643 ATCGAT AT^CGAT
DrdI 3451 GACACCTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 910 AGCGCT AGC^GCT
EcoNI 809 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2518 TGATCA T^GATCA
FspI 1066 TGCGCA TGC^GCA
HincII 4333 GTTGAC GT[CT]^[GA]AC
HindII 4333 GTTGAC GT[CT]^[GA]AC
KpnI 2894 GGTACC GGTAC^C
PaeR7I 1383 CTCGAG C^TCGAG
PvuI 1139 CGATCG CGAT^CG
RcaI 610 TCATGA T^CATGA
SbfI 809 CCTGCAGG CCTGCA^GG
SmaI 1113 CCCGGG CCC^GGG
Sse8387I 809 CCTGCAGG CCTGCA^GG
SspBI 4198 TGTACA T^GTACA
Tsp45I 2962 GTGAC ^GT[GC]AC
XhoI 1383 CTCGAG C^TCGAG
XmaCI 1113 CCCGGG C^CCGGG
XmaI 1113 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AocI 572 CCTTAGG CC^T[ACGT]AGG
2321 CCTGAGG
ApaI 1032 GGGCCC GGGCC^C
1116 GGGCCC
Asp700I 1343 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1841 GAAACACTTC
AvaI 1113 CCCGGG C^[CT]CG[GA]G
1383 CTCGAG
BcgI 514 GCAGGAAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1186 GCAAGACCATCG
BciVI 2843 GTATCC GTATCC(6/5)
4290 GTATCC
BglII 278 AGATCT A^GATCT
3876 AGATCT
BsaBI 3865 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3877 GATCTCCATC
BsmBI 3852 CGTCTC CGTCTC(1/5)
672 GAGACG
BsmI 2536 GCATTC GAATGC(1/-1)
4015 GCATTC
BsoBI 1113 CCCGGG C^[CT]CG[GA]G
1383 CTCGAG
BsrBI 606 CCGCTC CCGCTC(-3/-3)
1163 CCGCTC
BstBI 2428 TTCGAA TT^CGAA
2864 TTCGAA
Bsu36I 572 CCTTAGG CC^T[ACGT]AGG
2321 CCTGAGG
Eco81I 572 CCTTAGG CC^T[ACGT]AGG
2321 CCTGAGG
EcoT14I 1873 CCATGG C^C[AT][AT]GG
3385 CCATGG
HgaI 963 GACGC GACGC(5/10)
975 GACGC
HindIII 1801 AAGCTT A^AGCTT
2025 AAGCTT
KspI 941 CCGCGG CCGC^GG
1409 CCGCGG
MaeII 694 ACGT A^CGT
1246 ACGT
MamI 3865 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3877 GATCTCCATC
NcoI 1873 CCATGG C^CATGG
3385 CCATGG
NspV 2428 TTCGAA TT^CGAA
2864 TTCGAA
PpuMI 1258 GGGACCT [GA]G^G[AT]CC[CT]
2509 GGGACCC
SacII 941 CCGCGG CCGC^GG
1409 CCGCGG
SapI 1833 GAAGAGC GCTCTTC(1/4)
2004 GAAGAGC
SfuI 2428 TTCGAA TT^CGAA
2864 TTCGAA
StyI 1873 CCATGG C^C[AT][AT]GG
3385 CCATGG
XmnI 1343 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1841 GAAACACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BanI BanII BbeI
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssHII
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI SspI StuI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII