##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AvaI AvaII AviII AvrII
BalI BamHI BciVI BglII BlnI
BseAI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BsrBI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I ClaI CpoI DraII DraIII
DrdI EaeI EagI EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRV FseI
FspI HpaI KpnI MluI MluNI
MroI MscI NaeI NcoI NgoMIV
NotI NruI PacI PaeR7I PmeI
PpuMI PshAI Psp1406I PstI PvuI
PvuII RsrII SalI SbfI ScaI
SexAI SfiI SgrAI SmaI SnaBI
SphI Sse8387I SspBI StuI SwaI
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 73 enzymes) #
###################################
AcyI 155 GGCGCC G[GA]^CG[CT]C
AflII 1256 CTTAAG C^TTAAG
AgeI 1547 ACCGGT A^CCGGT
AhdI 606 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3080 CCTAAGG CC^T[ACGT]AGG
AseI 2255 ATTAAT AT^TAAT
AsnI 2255 ATTAAT AT^TAAT
AspEI 606 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 905 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 155 GGCGCC G^G[CT][GA]CC
BbeI 155 GGCGCC GGCGC^C
BbrPI 1965 CACGTG CAC^GTG
BbvCI 3467 GCTGAGG CCTCAGC(-5/-2)
BcnI 132 CCGGG CC^[GC]GG
BfrI 1256 CTTAAG C^TTAAG
BglI 175 GCCGACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 206 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 206 GCTGAGC GC^T[ACGT]AGC
BsaAI 1965 CACGTG [CT]AC^GT[GA]
BsaBI 3623 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 155 GGCGCC G[GA]^CG[CT]C
BsaI 1163 GAGACC GGTCTC(1/5)
BsaWI 1547 ACCGGT [AT]^CCGG[AT]
BsiEI 1991 CGACCG CG[GA][CT]^CG
BsmI 810 GCATTC GAATGC(1/-1)
BspHI 253 TCATGA T^CATGA
BspLU11I 2852 ACATGT A^CATGT
BspMI 1597 ACCTGC ACCTGC(4/8)
BsrFI 1547 ACCGGT [GA]^CCGG[CT]
BstBI 1953 TTCGAA TT^CGAA
BstNI 3926 CCAGG CC^[AT]GG
BstXI 843 CCACAATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3080 CCTAAGG CC^T[ACGT]AGG
CelII 206 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1547 ACCGGT [GA]^CCGG[CT]
Cfr13I 4197 GGCCC G^G[ACGT]CC
DsaI 28 CCGCGG C^C[GA][CT]GG
Eam1105I 606 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3080 CCTAAGG CC^T[ACGT]AGG
EcoNI 2206 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 3926 CCAGG ^CC[AT]GG
EcoT14I 823 CCTTGG C^C[AT][AT]GG
HaeII 155 GGCGCC [GA]GCGC^[CT]
HgaI 518 GCGTC GACGC(5/10)
Hin1I 155 GGCGCC G[GA]^CG[CT]C
KasI 155 GGCGCC G^GCGCC
KspI 28 CCGCGG CCGC^GG
MamI 3623 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 450 CAATTG C^AATTG
MunI 450 CAATTG C^AATTG
MvaI 3926 CCAGG CC^[AT]GG
NarI 155 GGCGCC GG^CGCC
NciI 132 CCGGG CC^[GC]GG
NdeI 3252 CATATG CA^TATG
NheI 2197 GCTAGC G^CTAGC
NspV 1953 TTCGAA TT^CGAA
PflFI 905 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 563 CCAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1547 ACCGGT A^CCGGT
PmaCI 1965 CACGTG CAC^GTG
PmlI 1965 CACGTG CAC^GTG
PshBI 2255 ATTAAT AT^TAAT
RcaI 253 TCATGA T^CATGA
SacI 2392 GAGCTC GAGCT^C
SacII 28 CCGCGG CCGC^GG
SapI 396 GCTCTTC GCTCTTC(1/4)
Sau96I 4197 GGCCC G^G[ACGT]CC
SfoI 155 GGCGCC GGC^GCC
SfuI 1953 TTCGAA TT^CGAA
SpeI 1040 ACTAGT A^CTAGT
StyI 823 CCTTGG C^C[AT][AT]GG
Tth111I 905 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 563 CCAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccII 29 CGCG CG^CG
137 CGCG
AfaI 1545 GTAC GT^AC
2864 GTAC
AflIII 1966 ACGTGT A^C[GA][CT]GT
2852 ACATGT
Asp700I 2337 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3288 GAAAGAGTTC
AspHI 1496 GAGCAC G[AT]GC[AT]^C
2392 GAGCTC
BanII 209 GAGCCC G[GA]GC[CT]^C
2392 GAGCTC
BcgI 8 GCAGCGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
117 GCATTTCTATCG
BclI 1708 TGATCA T^GATCA
3628 TGATCA
BsgI 592 CTGCAC GTGCAG(16/14)
242 GTGCAG
BsiHKAI 1496 GAGCAC G[AT]GC[AT]^C
2392 GAGCTC
BsrDI 654 CATTGC GCAATG(2/0)
314 GCAATG
BssKI 132 CCGGG ^CC[ACGT]GG
3926 CCAGG
BstAPI 553 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
801 GCAAGAAATGC
BstUI 29 CGCG CG^CG
137 CGCG
BstYI 1958 AGATCC [GA]^GATC[CT]
3863 GGATCT
DraI 1201 TTTAAA TTT^AAA
2293 TTTAAA
EcoRI 2514 GAATTC G^AATTC
3634 GAATTC
EcoT22I 808 ATGCAT ATGCA^T
1502 ATGCAT
FauI 138 GCGGG CCCGC(4/6)
2306 GCGGG
FbaI 1708 TGATCA T^GATCA
3628 TGATCA
HaeIII 59 GGCC GG^CC
4197 GGCC
HapII 132 CCGG C^CGG
1548 CCGG
HincII 980 GTTGAC GT[CT]^[GA]AC
3193 GTCAAC
HindII 980 GTTGAC GT[CT]^[GA]AC
3193 GTCAAC
HindIII 248 AAGCTT A^AGCTT
2762 AAGCTT
HpaII 132 CCGG C^CGG
1548 CCGG
MaeII 1249 ACGT A^CGT
1966 ACGT
MflI 1958 AGATCC [GA]^GATC[CT]
3863 GGATCT
MslI 844 CACAATAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1505 CATCAGAATG
MspI 132 CCGG C^CGG
1548 CCGG
MvnI 29 CGCG CG^CG
137 CGCG
NsiI 808 ATGCAT ATGCA^T
1502 ATGCAT
RsaI 1545 GTAC GT^AC
2864 GTAC
ScrFI 132 CCGGG CC^[ACGT]GG
3926 CCAGG
SspI 1772 AATATT AAT^ATT
3336 AATATT
XhoII 1958 AGATCC [GA]^GATC[CT]
3863 GGATCT
XmnI 2337 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3288 GAAAGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BstF5I
Cac8I CfoI DdeI DpnII EarI
Fnu4HI FokI HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MspA1I
MwoI NdeII NlaIII NlaIV NspI
PleI Sau3AI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I