##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AhdI Aor51HI ApaI AscI AspEI
AspI AvaI AviII BamHI BanII
BbrPI BbvCI BcgI BglI BlpI
Bpu1102I BsaAI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I FseI
FspI HindIII HpaI KspI MluI
MroI MvnI NaeI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PshAI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 3109 GGTACC G^GTACC
AcyI 308 GGCGCC G[GA]^CG[CT]C
AflII 2248 CTTAAG C^TTAAG
AgeI 2343 ACCGGT A^CCGGT
AocI 149 CCTCAGG CC^T[ACGT]AGG
AseI 608 ATTAAT AT^TAAT
AsnI 608 ATTAAT AT^TAAT
Asp718I 3109 GGTACC G^GTACC
AvaII 218 GGTCC G^G[AT]CC
AvrII 3486 CCTAGG C^CTAGG
BalI 1720 TGGCCA TGG^CCA
BbeI 308 GGCGCC GGCGC^C
BcnI 1 CCCGG CC^[GC]GG
BfrI 2248 CTTAAG C^TTAAG
BglII 379 AGATCT A^GATCT
BlnI 3486 CCTAGG C^CTAGG
BsaBI 2096 GATAGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 308 GGCGCC G[GA]^CG[CT]C
BsaI 2174 GGTCTC GGTCTC(1/5)
BseRI 1593 GAGGAG GAGGAG(10/8)
BspHI 1714 TCATGA T^CATGA
BsrFI 2343 ACCGGT [GA]^CCGG[CT]
Bsu36I 149 CCTCAGG CC^T[ACGT]AGG
CfoI 309 GCGC GCG^C
Cfr10I 2343 ACCGGT [GA]^CCGG[CT]
Cfr13I 218 GGTCC G^G[ACGT]CC
DraII 217 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 1441 CCATGG C^C[GA][CT]GG
EaeI 1720 TGGCCA [CT]^GGCC[GA]
Eco81I 149 CCTCAGG CC^T[ACGT]AGG
EcoO109I 217 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1916 GATATC GAT^ATC
HaeII 308 GGCGCC [GA]GCGC^[CT]
HgaI 1977 GACGC GACGC(5/10)
HhaI 309 GCGC GCG^C
Hin1I 308 GGCGCC G[GA]^CG[CT]C
HinP1I 309 GCGC G^CGC
HincII 2860 GTTGAC GT[CT]^[GA]AC
HindII 2860 GTTGAC GT[CT]^[GA]AC
KasI 308 GGCGCC G^GCGCC
KpnI 3109 GGTACC GGTAC^C
MamI 2096 GATAGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1720 TGGCCA TGG^CCA
MscI 1720 TGGCCA TGG^CCA
MspA1I 143 CCGCTG C[AC]G^C[GT]G
NarI 308 GGCGCC GG^CGCC
NciI 1 CCCGG CC^[GC]GG
NcoI 1441 CCATGG C^CATGG
NheI 3145 GCTAGC G^CTAGC
PinAI 2343 ACCGGT A^CCGGT
PpuMI 217 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 608 ATTAAT AT^TAAT
RcaI 1714 TCATGA T^CATGA
Sau96I 218 GGTCC G^G[ACGT]CC
ScaI 1831 AGTACT AGT^ACT
SfoI 308 GGCGCC GGC^GCC
StuI 1621 AGGCCT AGG^CCT
XbaI 3797 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 225 GTGCAC G^TGCAC
672 GTGCAC
ApaLI 225 GTGCAC G^TGCAC
672 GTGCAC
Asp700I 431 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2730 GAATTCCTTC
AspHI 225 GTGCAC G[AT]GC[AT]^C
672 GTGCAC
BanI 308 GGCGCC G^G[CT][GA]CC
3109 GGTACC
BbsI 1907 GAAGAC GAAGAC(2/6)
2045 GAAGAC
BclI 1069 TGATCA T^GATCA
1333 TGATCA
BpuAI 1907 GAAGAC GAAGAC(2/6)
2045 GAAGAC
BsaWI 1845 ACCGGA [AT]^CCGG[AT]
2343 ACCGGT
BsiHKAI 225 GTGCAC G[AT]GC[AT]^C
672 GTGCAC
BsrBI 155 GAGCGG CCGCTC(-3/-3)
173 GAGCGG
DraI 2800 TTTAAA TTT^AAA
3901 TTTAAA
FauI 28 CCCGC CCCGC(4/6)
175 GCGGG
FbaI 1069 TGATCA T^GATCA
1333 TGATCA
MfeI 1692 CAATTG C^AATTG
3114 CAATTG
MunI 1692 CAATTG C^AATTG
3114 CAATTG
NdeI 1928 CATATG CA^TATG
2536 CATATG
NlaIV 308 GGCGCC GG[ACGT]^[ACGT]CC
3109 GGTACC
PstI 192 CTGCAG CTGCA^G
1107 CTGCAG
XcmI 1441 CCATGGGCAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2884 CCAAAAAGAAAGTGG
XmnI 431 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2730 GAATTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI BbvI BciVI
BfaI BmyI BpmI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspLU11I BspMI BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I DdeI
DpnII EarI EcoRI EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII NsiI
NspI PleI RsaI Sau3AI ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII