##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII Aor51HI ApaI AscI AseI
AsnI Asp718I AspI AvaI AviII
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BsaAI BsaHI BsiEI
BsiWI BsoBI Bsp1407I BsrBI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CpoI DrdI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
FseI FspI HaeII Hin1I KasI
KpnI KspI MfeI MluI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 3229 GTCTAC GT^[AC][GT]AC
AgeI 250 ACCGGT A^CCGGT
AhdI 706 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 298 GTGCAC G^TGCAC
AocI 2785 CCTGAGG CC^T[ACGT]AGG
ApaLI 298 GTGCAC G^TGCAC
AspEI 706 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1427 CCTAGG C^CTAGG
BalI 3070 TGGCCA TGG^CCA
BamHI 1863 GGATCC G^GATCC
BanI 2091 GGTGCC G^G[CT][GA]CC
BbsI 826 GAAGAC GAAGAC(2/6)
BclI 353 TGATCA T^GATCA
BcnI 1855 CCGGG CC^[GC]GG
BglII 3550 AGATCT A^GATCT
BlnI 1427 CCTAGG C^CTAGG
BlpI 2145 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2145 GCTCAGC GC^T[ACGT]AGC
BpuAI 826 GAAGAC GAAGAC(2/6)
BsaBI 3545 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1514 CGTCTC CGTCTC(1/5)
BspDI 325 ATCGAT AT^CGAT
BspHI 3903 TCATGA T^CATGA
BspMI 2284 ACCTGC ACCTGC(4/8)
BsrDI 276 GCAATG GCAATG(2/0)
BsrFI 250 ACCGGT [GA]^CCGG[CT]
Bsu36I 2785 CCTGAGG CC^T[ACGT]AGG
CelII 2145 GCTCAGC GC^T[ACGT]AGC
CfoI 228 GCGC GCG^C
Cfr10I 250 ACCGGT [GA]^CCGG[CT]
ClaI 325 ATCGAT AT^CGAT
DraII 872 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 3070 TGGCCA [CT]^GGCC[GA]
Eam1105I 706 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2785 CCTGAGG CC^T[ACGT]AGG
EcoO109I 872 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 328 GATATC GAT^ATC
FauI 731 GCGGG CCCGC(4/6)
FbaI 353 TGATCA T^GATCA
HhaI 228 GCGC GCG^C
HinP1I 228 GCGC G^CGC
HpaI 3179 GTTAAC GTT^AAC
MamI 3545 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3070 TGGCCA TGG^CCA
MscI 3070 TGGCCA TGG^CCA
NciI 1855 CCGGG CC^[GC]GG
NcoI 443 CCATGG C^CATGG
PinAI 250 ACCGGT A^CCGGT
PstI 688 CTGCAG CTGCA^G
RcaI 3903 TCATGA T^CATGA
ScaI 2649 AGTACT AGT^ACT
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 27 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
1726 CAGCTGCTG
Asp700I 2259 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2382 GAAAGACTTC
BsaI 843 GAGACC GGTCTC(1/5)
1669 GAGACC
BsmI 3401 GAATGC GAATGC(1/-1)
2336 GCATTC
BssSI 1716 CACGAG CACGAG(-5/-1)
399 CTCGTG
BstAPI 1725 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2150 GCATAGGTTGC
BstXI 1561 CCACTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2221 CCAAACCCTTGG
DraIII 103 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1931 CACTTTGTG
EcoT22I 155 ATGCAT ATGCA^T
3403 ATGCAT
HgaI 29 GACGC GACGC(5/10)
342 GACGC
HindIII 1391 AAGCTT A^AGCTT
2590 AAGCTT
MspA1I 1726 CAGCTG C[AC]G^C[GT]G
3297 CAGCTG
NsiI 155 ATGCAT ATGCA^T
3403 ATGCAT
PvuII 1726 CAGCTG CAG^CTG
3297 CAGCTG
SfcI 688 CTGCAG C^T[GA][CT]AG
1028 CTACAG
SspI 1067 AATATT AAT^ATT
2262 AATATT
StuI 1302 AGGCCT AGG^CCT
1430 AGGCCT
XcmI 917 CCAACACATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1187 CCATCTACTCTCTGG
XmnI 2259 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2382 GAAAGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccIII AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaII
BanII BbvI BfaI BmyI BpmI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspEI BspLU11I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DsaI EarI
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MroI MseI MslI MspI
MvaI MwoI NdeI NdeII NlaIII
NlaIV NspI PleI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII