##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI Asp700I
AspEI AviII AvrII BclI BfrI
BlnI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI Eam1105I Eco47III
EcoRV FbaI FseI FspI KspI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SapI
SbfI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 1724 GACGTC GACGT^C
Acc65I 1077 GGTACC G^GTACC
AccII 102 CGCG CG^CG
AcsI 1971 GAATTC [GA]^AATT[CT]
AflIII 1098 ACGTGT A^C[GA][CT]GT
Alw44I 3939 GTGCAC G^TGCAC
AocI 816 CCTCAGG CC^T[ACGT]AGG
ApaLI 3939 GTGCAC G^TGCAC
ApoI 1971 GAATTC [GA]^AATT[CT]
Asp718I 1077 GGTACC G^GTACC
BbrPI 3189 CACGTG CAC^GTG
BciVI 2387 GTATCC GTATCC(6/5)
BsaBI 1444 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 172 CGGCCG CG[GA][CT]^CG
BsmBI 3237 CGTCTC CGTCTC(1/5)
BsrDI 2358 CATTGC GCAATG(2/0)
BstUI 102 CGCG CG^CG
Bsu36I 816 CCTCAGG CC^T[ACGT]AGG
EagI 172 CGGCCG C^GGCCG
EclXI 172 CGGCCG C^GGCCG
Eco52I 172 CGGCCG C^GGCCG
Eco81I 816 CCTCAGG CC^T[ACGT]AGG
EcoNI 1126 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1971 GAATTC G^AATTC
HindIII 3090 AAGCTT A^AGCTT
HpaI 2110 GTTAAC GTT^AAC
KpnI 1077 GGTACC GGTAC^C
MamI 1444 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 102 CGCG CG^CG
PmaCI 3189 CACGTG CAC^GTG
PmlI 3189 CACGTG CAC^GTG
SalI 1981 GTCGAC G^TCGAC
ScaI 1355 AGTACT AGT^ACT
SexAI 927 ACCTGGT A^CC[AT]GGT
SfiI 1238 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1049 CTCAAG C^T[CT][GA]AG
SphI 1177 GCATGC GCATG^C
StuI 2087 AGGCCT AGG^CCT
Tsp509I 1972 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1981 GTCGAC GT^[AC][GT]AC
2405 GTCTAC
ApaI 208 GGGCCC GGGCC^C
2799 GGGCCC
AspI 278 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3151 GACGGCGTC
BbeI 1326 GGCGCC GGCGC^C
1930 GGCGCC
BbvCI 2628 GCTGAGG CCTCAGC(-5/-2)
4317 GCTGAGG
BcgI 1374 CGATGACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2749 CGAGCCTGGTGC
BglII 1478 AGATCT A^GATCT
3910 AGATCT
BsaAI 3189 CACGTG [CT]AC^GT[GA]
3942 CACGTA
BsaI 60 GAGACC GGTCTC(1/5)
1829 GAGACC
BspHI 302 TCATGA T^CATGA
2986 TCATGA
BsrFI 322 GCCGGC [GA]^CCGG[CT]
2065 GCCGGC
BstAPI 1271 GCAGAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3378 GCAGGGGCTGC
BstEII 192 GGTTACC G^GT[ACGT]ACC
2464 GGTGACC
BstPI 192 GGTTACC G^GT[ACGT]ACC
2464 GGTGACC
BstXI 1849 CCACTAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3570 CCACCTGTGTGG
Cfr10I 322 GCCGGC [GA]^CCGG[CT]
2065 GCCGGC
DraIII 1398 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
4197 CACACTGTG
EcoO65I 192 GGTTACC G^GT[ACGT]ACC
2464 GGTGACC
EcoT22I 383 ATGCAT ATGCA^T
1445 ATGCAT
KasI 1326 GGCGCC G^GCGCC
1930 GGCGCC
NaeI 322 GCCGGC GCC^GGC
2065 GCCGGC
NarI 1326 GGCGCC GG^CGCC
1930 GGCGCC
NcoI 790 CCATGG C^CATGG
3281 CCATGG
NgoMIV 322 GCCGGC G^CCGGC
2065 GCCGGC
NsiI 383 ATGCAT ATGCA^T
1445 ATGCAT
PflFI 278 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3151 GACGGCGTC
RcaI 302 TCATGA T^CATGA
2986 TCATGA
SfoI 1326 GGCGCC GGC^GCC
1930 GGCGCC
Tth111I 278 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3151 GACGGCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrI BssKI BssSI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI