##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Aor51HI AscI AseI AsnI Asp718I
AviII BbeI BfrI BsiEI BsmI
Bsp1407I BsrBI BsrDI BsrGI BssSI
Bst1107I BstBI BstZ17I CpoI DraI
DrdI EagI EclXI Eco47III Eco52I
EcoRV FseI FspI KasI KpnI
KspI MfeI MunI NarI NdeI
NheI NotI NruI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 470 GTAGAC GT^[AC][GT]AC
AccIII 1770 TCCGGA T^CCGGA
AcyI 2591 GACGCC G[GA]^CG[CT]C
Asp700I 2254 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3590 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1142 CCTAGG C^CTAGG
BciVI 2973 GGATAC GTATCC(6/5)
BclI 2386 TGATCA T^GATCA
BglI 1703 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2694 AGATCT A^GATCT
BlnI 1142 CCTAGG C^CTAGG
BlpI 2563 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2563 GCTGAGC GC^T[ACGT]AGC
BsaBI 1861 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2591 GACGCC G[GA]^CG[CT]C
BsaWI 1770 TCCGGA [AT]^CCGG[AT]
BseAI 1770 TCCGGA T^CCGGA
BsiWI 2941 CGTACG C^GTACG
BspDI 1446 ATCGAT AT^CGAT
BspEI 1770 TCCGGA T^CCGGA
BspLU11I 180 ACATGT A^CATGT
BssHII 3727 GCGCGC G^CGCGC
BstAPI 3355 GCATAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1789 GGTGACC G^GT[ACGT]ACC
BstPI 1789 GGTGACC G^GT[ACGT]ACC
CelII 2563 GCTGAGC GC^T[ACGT]AGC
ClaI 1446 ATCGAT AT^CGAT
DraIII 1564 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1789 GGTGACC G^GT[ACGT]ACC
FbaI 2386 TGATCA T^GATCA
HaeII 633 GGCGCT [GA]GCGC^[CT]
Hin1I 2591 GACGCC G[GA]^CG[CT]C
HindIII 2327 AAGCTT A^AGCTT
HpaI 2266 GTTAAC GTT^AAC
MamI 1861 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 2164 ACGCGT A^CGCGT
MroI 1770 TCCGGA T^CCGGA
PaeR7I 3435 CTCGAG C^TCGAG
PflFI 3590 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 954 CTGCAG CTGCA^G
ScaI 3498 AGTACT AGT^ACT
SmlI 3435 CTCGAG C^T[CT][GA]AG
SphI 361 GCATGC GCATG^C
Tth111I 3590 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2344 TCTAGA T^CTAGA
XhoI 3435 CTCGAG C^TCGAG
XmnI 2254 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
ApaI 1701 GGGCCC GGGCC^C
3689 GGGCCC
BamHI 1585 GGATCC G^GATCC
3030 GGATCC
BbrPI 1331 CACGTG CAC^GTG
2390 CACGTG
BsaAI 1331 CACGTG [CT]AC^GT[GA]
2390 CACGTG
EcoNI 977 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1627 CCTCCCCGAGG
EcoRI 1918 GAATTC G^AATTC
2960 GAATTC
EcoT22I 359 ATGCAT ATGCA^T
841 ATGCAT
NaeI 568 GCCGGC GCC^GGC
2060 GCCGGC
NgoMIV 568 GCCGGC G^CCGGC
2060 GCCGGC
NsiI 359 ATGCAT ATGCA^T
841 ATGCAT
PmaCI 1331 CACGTG CAC^GTG
2390 CACGTG
PmlI 1331 CACGTG CAC^GTG
2390 CACGTG
PshAI 917 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1223 GACGTGGGTC
TaqI 1447 TCGA T^CGA
3436 TCGA
TthHB8I 1447 TCGA T^CGA
3436 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AhdI AluI Alw44I AlwI AlwNI
AocI ApaLI ApoI AspEI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PvuII RcaI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XhoII